Molecular Characterisation by Infrared Spectroscopy of Bordetella pertussis Grown as Biofilm

Bosch, Alejandra; Massa, N. E.; Donolo, Ana; Yantorno, Osvaldo

doi:10.1002/1521-3951(200007)220:1<635::aid-pssb635>3.0.co;2-l

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…Bordetella pertussis, a Gram-negative pathogen, which colonizes the respiratory tract and is responsible for whooping cough, has been the subject of a flurry of recently intensive studies of its infection mechanism, especially the initial phase that involves adherence of the microbes to epithelial tissues and their growth as a biofilm. Bosch et al (2000) used FT-IR to investigate how B. pertussis can grow attached to an abiotic surface and how this attachment can foster production of exopolymers that then lead to formation of a biofilm. Later on, Bosch et al (2006) studied the characterization of B. pertussis growing as a biofilm by FT-IR.…”

Section: Biofilm Detection By Vibrational Spectroscopysupporting

confidence: 50%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

204

120

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Infrared spectroscopy and Raman spectroscopy provide complementary technologies for rapid and precise detection of microorganisms and are emerging methods in food analysis. It is possible to use either of these techniques to differentiate and quantify microorganisms in relatively simple matrices such as liquid media and simple solutions with determinations taking less than an hour. Vibrational spectroscopy, unlike other techniques used in microbiology, is a relatively simple method for studying structural changes occurring within a microbial cell following environmental stress and applications of food processing treatments. Vibrational spectroscopy provides a wide range of biochemical properties about bacteria in a single spectrum, most importantly characteristics of the cell membrane. These techniques are especially useful for studying properties of bacterial biofilms on contact surfaces, the presence and viability of bacterial vegetative cells and spores, the type and degree of bacterial injury, and assessment of antibiotic susceptibility. Future trends in food analysis will involve combining vibrational spectroscopy with microscopy, mass spectroscopy, or DNA-based methods to comprehensively study bacterial stress. Further advances in selectivity, sensitivity, and improved chemometric methods, along with reduction in the cost of instrumentation, may lead to the development of fieldready and real-time analytical systems.

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Section: Biofilm Detection By Vibrational Spectroscopysupporting

confidence: 50%

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Al-Qadiri

Lin

et al. 2011

Food Bioprocess Technol

204

120

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“…So far little is known about the mechanism adopted by the bacteria to survive and persist in humans. Recent studies have provided evidence that bacteria of the Bordetella genus may undergo a community-based existence (biofilm), which might constitute a strategy for them to colonize and persist within the host [10][11][12][13]. Biofilm is a microbial lifestyle where organisms grow on surfaces in organized communities.…”

Section: Introductionmentioning

confidence: 99%

Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques

et al. 2007

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This work describes the application of several analytical techniques to characterize the development of Bordetella pertussis biofilms and to examine, in particular, the contribution of virulence factors in this development. Growth of surface-attached virulent and avirulent B. pertussis strains was monitored in continuous-flow chambers by techniques such as the crystal violet method, and nondestructive methodologies like fluorescence microscopy and Fourier transform (FT) IR spectroscopy. Additionally, B. pertussis virulent and avirulent strains expressing green fluorescent protein were grown adhered to the base of a glass chamber of 1-μm thickness. Three-dimensional images of mature biofilms, acquired by confocal laser scanning microscopy, were quantitatively analysed by means of the computer program COMSTAT. Our results indicate that only the virulent (Bvg + ) phase of B. pertussis is able to attach to surfaces and develop a mature biofilm. In the virulent phase these bacteria are capable of producing a biofilm consisting of microcolonies of approximately 200 μm in diameter and 24 μm in depth. FTIR spectroscopy allowed us not only to follow the dynamics of biofilm growth through specific biomass and biofilm marker absorption bands, but also to monitor the maturation of the biofilm by means of the increase of the carbohydrate-to-protein ratio.

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Investigating Food Spoilage and Pathogenic Microorganisms by Mid‐Infrared Spectroscopy

Rasco

2001

Handbook of Vibrational Spectroscopy

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Mid‐infrared (mid‐IR) spectroscopy (4000–400 cm −1 ) has great potential for determining biochemical properties of microbes that are important to food quality and safety. For example, rapid and precise measurements of pathogenic and spoilage microorganisms present in food are possible, thanks to recent advances in detector technology and multivariate analysis. With mid‐IR spectrometry it is possible to determine compositional properties of bacterial cell membranes, providing useful information about the genotype as well as phenotypical characteristics. In general, the most significant spectral regions for evaluation are Amide I (∼1650 cm −1 ), Amide II (∼1540 cm −1 ), polysaccharide (1200–900 cm −1 ), nucleic acid (∼1240 and ∼1080 cm −1 ), and certain lipid features, such as ω‐cyclic fatty acids (∼2929 cm −1 ). Much research has been conducted to discriminate and quantify the specific microorganisms using this technique since the 1990s. In this chapter, we review recent work focusing on standardizing chemometric‐processing steps, investigations of bacterial biofilms, spore characterization, microbial injury, and bacterial growth. Advantages and disadvantages of the application of IR spectral techniques for microbiological research are also presented.

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Molecular Characterisation by Infrared Spectroscopy of Bordetella pertussis Grown as Biofilm

Cited by 9 publications

References 18 publications

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Application of Mid-infrared and Raman Spectroscopy to the Study of Bacteria

Continuous nondestructive monitoring of Bordetella pertussis biofilms by Fourier transform infrared spectroscopy and other corroborative techniques

Investigating Food Spoilage and Pathogenic Microorganisms by Mid‐Infrared Spectroscopy

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