Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton

Zhou, Kexue; Long, Lu; Sun, Quan; Wang, Weina; Gao, Wei; Chu, Zongyan; Cai, Chaowei; Mo, Jianchuan; Cheng, Jieru; Zhang, Xiangrui; Liu, Yujia; Du, Xiongming; Miao, Chen; Shí, Yùzhēn; Yuán, Yǒulù; Zhang, Xiao; Cai, Yingfan

doi:10.1515/biolog-2017-0003

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Therefore, depending on the family member, both the overexpression and silencing of these genes could enhance the resistant response against V. dahliae. Indeed CYP77A2 overexpression increased resistance against V. dahliae thanks to its role in the biosynthesis of antifungal compounds [66], whereas the downregulation of CYP94C1, which is highly expressed in plant roots, improves resistance to Verticillium wilt in cotton [67]. Of the HR up-regulated cytochromes P450, two members of CYP71 and one of CYP72 were found, which are precisely two of the three families of the cytochromes P450 monoxygenases involved in diterpene biosynthesis [68].…”

Section: Discussionmentioning

confidence: 99%

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

et al. 2021

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Background Olive orchards are threatened by a wide range of pathogens. Of these, Verticillium dahliae has been in the spotlight for its high incidence, the difficulty to control it and the few cultivars that has increased tolerance to the pathogen. Disease resistance not only depends on detection of pathogen invasion and induction of responses by the plant, but also on barriers to avoid the invasion and active resistance mechanisms constitutively expressed in the absence of the pathogen. In a previous work we found that two healthy non-infected plants from cultivars that differ in V. dahliae resistance such as ‘Frantoio’ (resistant) and ‘Picual’ (susceptible) had a different root morphology and gene expression pattern. In this work, we have addressed the issue of basal differences in the roots between Resistant and Susceptible cultivars. Results The gene expression pattern of roots from 29 olive cultivars with different degree of resistance/susceptibility to V. dahliae was analyzed by RNA-Seq. However, only the Highly Resistant and Extremely Susceptible cultivars showed significant differences in gene expression among various groups of cultivars. A set of 421 genes showing an inverse differential expression level between the Highly Resistant to Extremely Susceptible cultivars was found and analyzed. The main differences involved higher expression of a series of transcription factors and genes involved in processes of molecules importation to nucleus, plant defense genes and lower expression of root growth and development genes in Highly Resistant cultivars, while a reverse pattern in Moderately Susceptible and more pronounced in Extremely Susceptible cultivars were observed. Conclusion According to the different gene expression patterns, it seems that the roots of the Extremely Susceptible cultivars focus more on growth and development, while some other functions, such as defense against pathogens, have a higher expression level in roots of Highly Resistant cultivars. Therefore, it seems that there are constitutive differences in the roots between Resistant and Susceptible cultivars, and that susceptible roots seem to provide a more suitable environment for the pathogen than the resistant ones.

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Section: Discussionmentioning

confidence: 99%

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

et al. 2021

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Physiological and molecular mechanism of defense in cotton against Verticillium dahliae

Shaban

Miao

Ullah

et al. 2018

Plant Physiology and Biochemistry

121

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Functional analysis of the <italic>GhCYP94C1</italic> gene in seed germination and flowering time of upland cotton

Yu¹

2022

Sci. Sin.-Vitae

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Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton

Cited by 3 publications

References 53 publications

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

Physiological and molecular mechanism of defense in cotton against Verticillium dahliae

Functional analysis of the <italic>GhCYP94C1</italic> gene in seed germination and flowering time of upland cotton

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Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton

Cited by 3 publications

References 53 publications

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots

Physiological and molecular mechanism of defense in cotton against Verticillium dahliae

Functional analysis of the &lt;italic&gt;GhCYP94C1&lt;/italic&gt; gene in seed germination and flowering time of upland cotton

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Functional analysis of the <italic>GhCYP94C1</italic> gene in seed germination and flowering time of upland cotton