Molecular Chaperone ERp29: A Potential Target for Cellular Protection in Retinal and Neurodegenerative Diseases

McLaughlin, Todd; Falkowski, Marek; Wang, Joshua J.; Zhang, Sarah X.

doi:10.1007/978-3-319-75402-4_52

Cited by 21 publications

(13 citation statements)

References 22 publications

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“…Hspa8 is also involved in mitochondrial protein import and β-amyloid (Aβ) clearance in the brain [61]. Erp29 is an endoplasmic reticulum (ER) luminal protein acting as a molecular chaperon in ER homeostasis, protein folding and trafficking, and cell survival [62]. A recent study indicated that Erp29 exerts neuroprotective effects through protecting neurons from apoptosis and promoting neuronal regeneration [63].…”

Section: Discussionmentioning

confidence: 99%

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

et al. 2019

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Rice (Oryza sativa L.) is the richest source of γ-oryzanol, a compound endowed with antioxidant and anti-inflammatory properties. γ-Oryzanol has been demonstrated to cross the blood-brain barrier in intact form and exert beneficial effects on brain function. This study aimed to clarify the effects of γ-oryzanol in the hippocampus in terms of cognitive function and protein expression. Adult mice were administered with γ-oryzanol 100 mg/kg or vehicle (control) once a day for 21 consecutive days following which cognitive behavior and hippocampal proteome were investigated. Cognitive tests using novel object recognition and Y-maze showed that long-term consumption of γ-oryzanol improves cognitive function in mice. To investigate the hippocampal proteome modulated by γ-oryzanol, 2D-difference gel electrophoresis (2D-DIGE) was performed. Interestingly, we found that γ-oryzanol modulates quantitative changes of proteins involved in synaptic plasticity and neuronal trafficking, neuroprotection and antioxidant activity, and mitochondria and energy metabolism. These findings suggested γ-oryzanol as a natural compound able to maintain and reinforce brain function. Although more intensive studies are needed, we propose γ-oryzanol as a putative dietary phytochemical for preserving brain reserve, the ability to tolerate age-related changes, thereby preventing clinical symptoms or signs of neurodegenerative diseases.

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Section: Discussionmentioning

confidence: 99%

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

et al. 2019

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“…While our GRP78 test verified that L-Z reduced the ERS, we are also interested in the expression of endoplasmic reticulum protein 29 (ERp29) which was investigated in recent retinal studies [67, 68]. ERp29, ubiquitously expressed in the ER membrane among various tissues and cell types, is an ER luminal protein in all mammals [69, 70].…”

Section: Discussionmentioning

confidence: 94%

“…ERp29, ubiquitously expressed in the ER membrane among various tissues and cell types, is an ER luminal protein in all mammals [69, 70]. It plays a pivotal role in modulating the folding and transportation of proteins during ERS [67]. GRP78 is one of the main targets of ERp29 [69].…”

Section: Discussionmentioning

confidence: 99%

Lutein and Zeaxanthin Isomers Reduce Photoreceptor Degeneration in the Pde6b^rd10 Mouse Model of Retinitis Pigmentosa

Yan

Beight

2018

BioMed Research International

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Purpose Lutein, RR-zeaxanthin, and RS-zeaxanthin (L-Z) are antioxidants which can reduce endoplasmic reticulum stress (ERS) and oxidative stress (OS), and ameliorate neurodegenerative diseases. However, their treatment effect in the Pde6brd10 (rd10) mouse model of retinitis pigmentosa (RP) and the underlying cellular mechanisms have not been studied. ERS is an important factor which causes photoreceptor apoptosis. The aim of the current project is to test the treatment effect of L-Z in rd10 mice and to investigate the underlying molecular mechanisms of ERS. Methods L-Z (Lutemax 2020, 10 mg/kg) diluted in sunflower oil (SFO, 1 mg/ml) or the same volume of SFO was administrated via gavage from postnatal day 6 (P6) to P20 daily in L-Z group (n=5) or SFO group (n=6) of rd10 mice. At P21, electroretinography (ERG) was performed to show the functional change of retinas. 78 kDa glucose-regulated protein (GRP78) and endoplasmic reticulum protein 29 (ERp29) were tested by western blot and immunostaining. Results The ERG amplitudes were larger in the L-Z group than those of the SFO group in all flash luminances of dark-adapted and light-adapted ERG (all p < 0.01). Western blot revealed that GRP78 in the retinas of the L-Z group was significantly downregulated compared to that of the SFO group (p < 0.01). Meanwhile, the retinal ERp29 protein was significantly upregulated in the L-Z treatment group than that of the SFO group (p < 0.01). Conclusions L-Z provide protection to the photoreceptors of rd10 mouse model of RP, which is probably associated with the reduction of ERS.

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“…[ 24 ] In retinal and neurodegenerative diseases, ERp29 affects cell survival and apoptosis. [ 25,26 ] In addition, ERp29 is a crucial factor involved in sperm fertilization via facilitating sperm‐oocyte membrane fusion. [ 27 ] The main findings of our study are as follows: the upregulation of ERp29 was identified in the severe pre‐eclamptic placentas, and the upregulation of ERp29 could block HTR‐8/SVneo cell metastasis, while downregulation of ERp29 had the opposite effect.…”

Section: Discussionmentioning

confidence: 99%

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR‐8/SVneo cells via modulating the epithelial‐mesenchymal transition

Zou

Dong

Zou

et al. 2020

J Biochem & Molecular Tox

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Dysfunction of trophoblast metastasis into the endometrium is the main cause of pre‐eclampsia (PE); however, the factors affecting this process are still unclear. In this study, we found that endoplasmic reticulum protein 29 (ERp29), one molecular chaperone of the endoplasmic reticulum, was aberrantly upregulated in the placenta of pre‐eclamptic patients compared with healthy controls. Then, an in vitro study using human extravillous trophoblast HTR‐8/SVneo cells showed that ERp29 upregulation could inhibit the migratory and invasive ability of HTR‐8/SVneo cells, while ERp29 downregulation had the opposite effect. Mechanical experiments confirmed that ERp29 blocked trophoblast metastasis via inhibiting the process of epithelial‐mesenchymal transition and affecting the Wnt/β‐catenin signaling pathway. In conclusion, this study revealed the important role of ERp29 in trophoblast metastasis and improved the mechanical understanding of PE occurrence.

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Molecular Chaperone ERp29: A Potential Target for Cellular Protection in Retinal and Neurodegenerative Diseases

Cited by 21 publications

References 22 publications

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

Lutein and Zeaxanthin Isomers Reduce Photoreceptor Degeneration in the Pde6b^rd10 Mouse Model of Retinitis Pigmentosa

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR‐8/SVneo cells via modulating the epithelial‐mesenchymal transition

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Molecular Chaperone ERp29: A Potential Target for Cellular Protection in Retinal and Neurodegenerative Diseases

Cited by 21 publications

References 22 publications

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice

Lutein and Zeaxanthin Isomers Reduce Photoreceptor Degeneration in the Pde6brd10 Mouse Model of Retinitis Pigmentosa

ERp29 affects the migratory and invasive ability of human extravillous trophoblast HTR‐8/SVneo cells via modulating the epithelial‐mesenchymal transition

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Lutein and Zeaxanthin Isomers Reduce Photoreceptor Degeneration in the Pde6b^rd10 Mouse Model of Retinitis Pigmentosa