Molecular changes in invasive front of oral cancer

Sharma, Mohit; Sah, Parul; Sharma, Sonal Soi; Radhakrishnan, Raghu

doi:10.4103/0973-029x.119740

Cited by 51 publications

(46 citation statements)

References 73 publications

(113 reference statements)

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“…However, the preparation of tissue samples for LC-MS/MS analysis can be challenging as a result of their morphological and molecular complexity 7,12,28 .…”

Section: Discussionmentioning

confidence: 99%

Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

et al. 2018

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Different regions of oral squamous cell carcinoma (OSCC) have particular histopathological and molecular characteristics limiting the standard tumor−node−metastasis prognosis classification. Therefore, defining biological signatures that allow assessing the prognostic outcomes for OSCC patients would be of great clinical significance. Using histopathology-guided discovery proteomics, we analyze neoplastic islands and stroma from the invasive tumor front (ITF) and inner tumor to identify differentially expressed proteins. Potential signature proteins are prioritized and further investigated by immunohistochemistry (IHC) and targeted proteomics. IHC indicates low expression of cystatin-B in neoplastic islands from the ITF as an independent marker for local recurrence. Targeted proteomics analysis of the prioritized proteins in saliva, combined with machine-learning methods, highlights a peptide-based signature as the most powerful predictor to distinguish patients with and without lymph node metastasis. In summary, we identify a robust signature, which may enhance prognostic decisions in OSCC and better guide treatment to reduce tumor recurrence or lymph node metastasis.

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“…However, the preparation of tissue samples for LC-MS/MS analysis can be challenging as a result of their morphological and molecular complexity 7,12,28 .…”

Section: Discussionmentioning

confidence: 99%

Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

et al. 2018

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“…The ITF, the deepest and most invasive area of the tumour, has been reported to contain useful prognostic information. In addition, various critical molecular events that regulate tumour proliferation, adhesion/migration, and angiogenesis occur in the ITF (Bankfalvi and Piffko, 2000; Rowe and Weiss, 2009; Sharma et al , 2013). Accordingly, the ITF is mainly responsible for the clinical behaviour of the tumour (Khanh do et al , 2011; Tanaka et al , 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The invasive front of tumours has been proposed to contain important prognostic information owing to the lack of cohesiveness, secretion of proteolytic enzymes, reorganisation of the extracellular matrix, and increased cell proliferation (Rowe and Weiss, 2009; Sharma et al , 2013). …”

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confidence: 99%

Prognostic impact of CD44-positive cancer stem-like cells at the invasive front of gastric cancer

et al. 2016

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Background:The invasive tumour front may provide prognostic information. We examined the relationship between the presence of cancer stem cells (CSCs) at the invasive tumour front and prognosis in gastric cancer (GC).Methods:CD44 is a CSC marker; accordingly, CD44 standard (CD44s), CD44 variant-6 (CD44v6), and CD44 variant-9 (CD44v9) expression were examined in 123 resected primary GCs and the clinical significance of CSCs at the invasive tumour front was analysed.Results:Thirteen (10.6%), 79 (64.2%), and 47 (38.2%) GCs were CD44s-, CD44v6-, and CD44v9-positive, respectively. Patients with CD44-positive expression at the invasive tumour front had significantly poorer disease-specific survival than those with negative expression (CD44s: P<0.00001, CD44v6: P=0.013, CD44v9: P=0.0002). CD44s expression at the invasive tumour front was an independent prognostic factor in resectable GC patients (hazard ratio=3.13; 95% confidence interval, 1.09–9.01; P=0.035) and was significantly associated with peritoneal (P<0.001), lymphatic (P<0.001), and haematogenous recurrences (P=0.008). In addition, the number of CD44 isoforms expressed in cancer cells at the invasive tumour front was associated with patient prognosis. No conventional clinicopathological factors were independently associated with CD44 expression at the invasive tumour front.Conclusions:CD44-positive cancer stem-like cells at the invasive tumour front indicate poor survival and can be a unique biological prognostic factor for GC.

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“…Este sistema considera que tumores pequenos sem propagação clínica têm melhor prognóstico do que tumores maiores com metástases. No entanto, alguns tumores com o mesmo estadiamento clínico, mostram diferentes padrões de crescimento e comportamento o que dificulta a descrição de um prognóstico com base apenas no seu estádio clínico (Sharma et al, 2013).…”

Section: Introductionunclassified

“…Alterações moleculares na frente invasiva dos tumores orais humanos foram documentadas por Sharma et al (2013) …”

Section: Introductionunclassified

Avaliação de margens cirúrgicas e recorrência local do carcinoma de célula escamosa oral de felino

Vasconcellos¹,

Diniz²

2015

Pubvet

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RESUMO. Quando o carcinoma de células escamosas orais (CCEO) apresenta recorrência em margens cirúrgicas histologicamente comprometidas, podemos facilmente explicá-la pela permanência de resíduos tumorais. Entretanto no caso de margens livres, sua progressão parece estar relacionada a presença de células geneticamente modificadas adjacentes ao tumor primário. Os diferentes métodos utilizados na avaliação das margens cirúrgicas, não são perfeitos ou isentos de limitações. Em Medicina Veterinária a análise histológica por congelamento intra-operatória, ainda apresenta restrições técnicas e econômicas limitando sua utilização. Estudos moleculares demonstram potencial para contribuir com um melhor prognóstico através da caracterização de um perfil gênico que permita uma avaliação de risco acurada. Este artigo revisa os métodos de análise de margens cirúrgicas, ressaltando a importância de modelos felinos na compreensão da biologia molecular do carcinoma oral humano. Palavras-chave: Carcinoma de células escamosas oral, exame histopatológico, análise molecular, felinosEvaluation of surgical border and local recurrence in oral squamous cell carcinoma in feline ABSTRACT. The recurrence of oral squamous cell carcinoma (OSCC) in compromised surgical border can be easily explained by the persistence of residual tumor. However, when the surgical borders are not compromised, the progression may be related to the presence of adjacent genetically modified cells generated by the primary tumor. The different methods used in the evaluation of surgical borders are not perfect and have limitations. In veterinary medicine, histological analysis by intraoperative frozen still has technical and economic restrictions limiting their use. Molecular studies demonstrate the potential contribution to a better prognosis through the characterization of a gene profile that allows a more accurate risk. This article reviews the methods of analysis of surgical borders, highlighting the importance of feline models to understand the molecular biology of human oral carcinoma.

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Molecular changes in invasive front of oral cancer

Cited by 51 publications

References 73 publications

Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

Combining discovery and targeted proteomics reveals a prognostic signature in oral cancer

Prognostic impact of CD44-positive cancer stem-like cells at the invasive front of gastric cancer

Avaliação de margens cirúrgicas e recorrência local do carcinoma de célula escamosa oral de felino

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