Molecular changes in fibrillar collagen in myxomatous mitral valve disease

Hadian, Mojtaba; Corcoran, Brendan; Bradshaw, Jeremy P.

doi:10.1016/j.carpath.2009.05.001

Cited by 29 publications

(30 citation statements)

References 35 publications

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“…In our samples, the denaturation endotherm was followed by an equilibrium state adequate to the new configuration of the protein molecules at temperatures just above 70°C. Similar values of denaturation temperatures are reported for samples from other collagenbased tissues [8,10,22,23]. Thermal stability of collagen molecules reflects the overall condition of the structure, cross-links in the collagen network, and its interactions with the surrounding molecules [4,21,[24][25][26].…”

Section: Discussionsupporting

confidence: 73%

“…The DSC method has proven to be a valuable tool in assessing the role of collagen in structural alterations of several cardiovascular and musculoskeletal diseases [8][9][10][11]. Our previous study showed that DSC was the appropriate method for investigation of subtle changes in physicochemical conditions of peritoneum using tissues obtained during surgical procedures [12].…”

Section: Introductionmentioning

confidence: 99%

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Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Torres

Trębacz

Pietrzyk

et al. 2017

J Therm Anal Calorim

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Obesity is considered to be the leading metabolic disease in the world. The coincidence of postoperative complications is higher in obese individuals; thus, laparoscopic techniques due to their minimally invasive character became a standard in the surgical treatment of obesity. The proper function of peritoneal tissue is essential for the response to surgical trauma. Therefore, understanding the alterations of peritoneal tissue of obese individuals might explain the increased number of postoperative complications observed in obese patients. The study aimed to assess whether the stability of peritoneum is altered in obese patients and whether it corresponds to early postoperative complications in obese patients. Samples of parietal peritoneal tissue were collected at the beginning of the laparoscopic cholecystectomy from 80 patients. Differential scanning calorimetry was performed on samples defrosted at room temperature. Patients were divided into three groups according to the BMI value. For statistical analysis, ANOVA, Chi-square, Student's t test, and correlations with Pearson test were used. Significantly lower DH and higher T m were in patients in whom intraperitoneal adhesions were reported. Lower DH was reported in samples obtained from patients with temperature above 37°C in the first 24 h postoperatively. Enthalpy of thermal transition (DH) in peritoneum obtained from parous was significantly lower in comparison with the samples from nullipara. It can be concluded that DSC parameters correlated with parameters related to the early postoperative period. Occurrence of adhesions was reported more frequently in the group of obese and overweight individuals and correlated with lower change in enthalpy of collagen denaturation.

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Section: Discussionsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Torres

Trębacz

Pietrzyk

et al. 2017

J Therm Anal Calorim

View full text Add to dashboard Cite

show abstract

“…DSC studies have proved to be valuable in assessing the role of collagen in structural alterations of aortic walls in abdominal aortic aneurysms and in mechanical alterations associated with mitral valve dysfunctions, as well as in the characterization of aortic tissues for cardiac valve bioprostheses and artificial blood vessels [16][17][18][19]. The calorimetric method has been successfully applied in investigations of collagen in mineralized bone tissue [11,15,20].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)

Torres¹,

Trębacz²,

Chróścicki³

et al. 2012

Folia Histochem Cytobiol.

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Abstract:Abdominal surgeries alter the integrity of the peritoneal layer and cause imbalances among immunological, inflammatory and angiogenic mechanisms within the tissue. During laparoscopic procedures a protective function of the peritoneal layer can be disturbed by the gas used to create a pneumoperitoneum. The aim of this study was to characterize peritoneal tissue by means of differential scanning calorimetry (DSC) as a reference for future investigations on the influence of surgical procedures on the physicochemical state of the peritoneum. Thirty-seven patients participated in the study. Patients were divided into three groups according to the type of surgery: group H -patients who underwent hernia repair; group Ch -patients who underwent laparoscopic cholecystectomy; and group C -patients operated due to rectal cancer. It was observed that onset temperature (T o ), denaturation temperature (T m ) and change of enthalpy (DH) during thermal denaturation of peritoneal collagen in were significantly different for these three groups of patients. The mean values of onset temperature (T o ) and denaturation temperature (T m ) in group H were significantly lower, while DH in this group was significantly higher than in the two other groups (Ch and C). This preliminary study does not answer whether the differences in collagen denaturation found in peritoneal tissue from different groups of patients resulted from a different inherent state of the tissue, or from surgical procedures. However, the results suggest that DSC is an appropriate method to study subtle changes in the physicochemical condition of the peritoneum using small samples obtained during surgical procedures.

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“…Until now, there is not a panel of diagnostic and prognostic biomarkers for sporadic forms, and their physiopathology remains unclear. Echocardiography evaluation represents until now unique "gold standard diagnostic" tool, even if it always reveals no appropriate for diagnosing moderate forms [3][4][5]. This underlines the necessity of innovative biomarker tools to identify in molecular pathways associated with mechanisms related to MVD pathogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…This underlines the necessity of innovative biomarker tools to identify in molecular pathways associated with mechanisms related to MVD pathogenesis. Recently, it is emerging that degenerative MVDs are the result of modified adult valve biology [4,5]. Particularly, Levine and colleagues have recently underlined that MVDs are the result of a 'living valve', which with advancing age shows active changes mediated both by valvular endothelial and interstitial cells and alterations in composition and turnover of elements of extracellular matrix [6].…”

Section: Introductionmentioning

confidence: 99%

Associations of rs3918242 and rs2285053 MMP-9 and MMP-2 polymorphisms with the risk, severity, and short- and long-term complications of degenerative mitral valve diseases: a 4.8-year prospective cohort study

Balistreri

Allegra

Crapanzano

et al. 2016

Cardiovascular Pathology

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Keywords:Degenerative forms of mitral valve diseases Metalloproteinases rs3918242, rs243865, rs2285053 MMP-2 and MMP-9 gene SNPs Management and outcome Background: Degenerative forms of mitral valve diseases (MVDs) are very complex pathologies. Thus, it is difficult to make generalizations about the disease pathways or genetic risk factors contributing to these diseases. However, a key role of metalloproteinases (MMPs) in their pathophysiology is emerging. Thus, we performed for the first time a perspective study to assess eventual associations of some functional single nucleotide polymorphisms (SNPs) in MMP-2 and MMP-9 genes with the MVD risk, symptom severity, and short-and long-term (4.8 years) complications. Materials and methods: For this purpose, 90 patients and two control groups were genotyped for rs3918242, rs243865, and rs2285053 MMP-2 and MMP-9 gene SNPs, and systemic levels of pro-atrial natriuretic peptide (pro-ANP) and two enzymes were quantified and correlated to genotypes of MMP-2 and MMP-9 SNPs studied. In addition, associations between these SNPs and symptom severity and short-and long-term (4.8 years) complications were evaluated. Results: Interestingly, rs3918242 MMP-9 and rs2285053 MMP-2 SNPs were significantly represented in cases than two control groups and were associated with a higher MVD risk, as demonstrated using dominant/recessive models. Cases stratified for NYHA symptoms and particularly those NYHA III+IV with rs3918242 CT+TT MMP-9 and rs2285053CT+TT genotypes also showed higher severity related to significant higher systemic levels of MMP enzymes and pro-ANP at enrolment and 4.8 follow-up times. In addition, cases with these genotypes and particularly those NYHA III+IV had a very significant percentage of complications, particularly at the 4.8 follow-up. Surprisingly, 20% of patient controls developed MVD at 4.8-year follow-up and were carriers of these genotypes. Conclusion: Thus, the associations observed seem to suggest that the two SNPs might represent useful biomarkers and targets for preventing and monitoring MVDs and developing personalized treatments, consenting a more appropriate management and outcome.

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Molecular changes in fibrillar collagen in myxomatous mitral valve disease

Cited by 29 publications

References 35 publications

Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Stability of peritoneal tissue evaluated by the means of DSC in obese patients undergoing laparoscopic cholecystectomy

Evaluation of peritoneal tissue by means of differential scanning calorimetry (DSC)

Associations of rs3918242 and rs2285053 MMP-9 and MMP-2 polymorphisms with the risk, severity, and short- and long-term complications of degenerative mitral valve diseases: a 4.8-year prospective cohort study

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