Molecular Breeding of Tomato Lines for Mass Production of Miraculin in a Plant Factory

Kato, Kazuhisa; Yoshida, Riku; Kikuzaki, Ayako; Hirai, Tadayoshi; Kawakubo, Hirofumi; Hiwasa-Tanase, Kyoko; Takane, Kenichi; Ezura, Hiroshi; Mizoguchi, Tsuyoshi

doi:10.1021/jf101874b

Cited by 50 publications

(33 citation statements)

References 27 publications

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“…One especially exciting example is the breeding of transgenic tomato lines that express the miraculin protein, a glycoprotein produced in fruits of the West African shrub Richadella dulcifica (Kato et al 2010). This protein functions as a sweetener under acidic conditions and is proposed as a potential sugar substitute in some foods.…”

Section: Nutrients and Neutraceuticalsmentioning

confidence: 99%

Breeding new varieties for controlled environments

Folta

2018

Plant Biol J

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Agricultural production in controlled environments is increasingly feasible, and may play an important role in providing nutrition and choice to growing urban centres. New technologies in lighting, ventilation, robotics and irrigation are just a few of the innovations that enable production of high-value specialty crops outside of a traditional field setting. However, despite all of the advances in the hardware within the plant factory operation, innovation of the most complex machine has been neglected - the plant itself. Indoor agricultural operations typically rely on legacy varieties, plants selected and bred for field conditions. In the field, phenotypic stability is paramount, as production must be consistent in an unpredictable and changing environment. However, the controlled environment affords focus on different breeding priorities as environmental flux, pests, pathogens and post-harvest quality are less formidable barriers to production. On the contrary, breeding for controlled environments shifts the focus to a completely different set of plant traits, such as rapid growth, performance in low light environments and active manipulation of plant stature. Instead of breeding for phenotypic stability, plants may be bred to maximise genetic plasticity, allowing specific traits to be presented as a function of the quality of the ambient light spectrum. In this scenario plant varieties may be grown with optimal size, supporting a focus on consumer traits like flavour or accumulation of health-related compounds. Gene editing may be a central technology in the production of designer plants for controlled environments. This review considers the opportunity for breeding for controlled environments, with a focus on a revision of priorities for controlled-environment breeders.

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Section: Nutrients and Neutraceuticalsmentioning

confidence: 99%

Breeding new varieties for controlled environments

Folta

2018

Plant Biol J

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“…To detect miraculin mRNA and recombinant miraculin accumulation in different parts of transgenic tomatoes, the fruits from the T 0 generation were separated into three tissues: exocarp, mesocarp, and others, including dissepiment, placenta, and jelly, according to the methods described by Kim et al (2010) and Kato et al (2010).…”

Section: Tissue Separation Of Transgenic Tomato Fruitsmentioning

confidence: 99%

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

et al. 2011

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The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.

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“…These growth systems expand crop production into the vertical dimension to produce a higher yield using less floor area (Hochmuth and Hochmuth 2001;Resh 2012). Examples of VFS include the use of vertical columns (Linsley-Noakes et al 2006), vertically suspended grow bags (Neocleous et al 2010), conveyor-driven stacked growth systems (Mahdavi et al 2012), A-frame designs (Hayden 2006), and plant factory approaches (Kato et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Vertical farming increases lettuce yield per unit area compared to conventional horizontal hydroponics

Touliatos

Dodd

McAinsh

2016

Food and Energy Security

193

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Vertical farming systems (VFS) have been proposed as an engineering solution to increase productivity per unit area of cultivated land by extending crop production into the vertical dimension. To test whether this approach presents a viable alternative to horizontal crop production systems, a VFS (where plants were grown in upright cylindrical columns) was compared against a conventional horizontal hydroponic system (HHS) using lettuce (Lactuca sativa L. cv. “Little Gem”) as a model crop. Both systems had similar root zone volume and planting density. Half‐strength Hoagland's solution was applied to plants grown in perlite in an indoor controlled environment room, with metal halide lamps providing artificial lighting. Light distribution (photosynthetic photon flux density, PPFD) and yield (shoot fresh weight) within each system were assessed. Although PPFD and shoot fresh weight decreased significantly in the VFS from top to base, the VFS produced more crop per unit of growing floor area when compared with the HHS. Our results clearly demonstrate that VFS presents an attractive alternative to horizontal hydroponic growth systems and suggest that further increases in yield could be achieved by incorporating artificial lighting in the VFS.

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Molecular Breeding of Tomato Lines for Mass Production of Miraculin in a Plant Factory

Cited by 50 publications

References 27 publications

Breeding new varieties for controlled environments

Breeding new varieties for controlled environments

Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

Vertical farming increases lettuce yield per unit area compared to conventional horizontal hydroponics

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