Molecular Biomarkers of Al3+ Effects on Induction of Oxidative Stress and Cellular Reactivation in Organism of Lepomis gibbosus (Pisces: Centrarchidae)

Novitskyi, R. O.; Sukharenko, Ye. V.; Nedzvetskiy, Victor S.

doi:10.1615/hydrobj.v50.i2.40

Cited by 4 publications

(8 citation statements)

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“…A little increased degree of variability of the bilateral features of common sunfish from the lower section of the Samara River (see figure) can be explained by two reasons: a) the presence of significant negative pressure on the water of river; b) significant flexibility of the non-indigenous species L. gibbosus, which is currently undergoing a rapid period of naturalization in the Dnipro region reservoirs (Novitskiy et al, 2014;Hubanova, 2019).…”

Section: Discussionmentioning

confidence: 99%

Fluctuating fish asymmetry in natural and artificial reservoirs of Dnipro region on example of invasion types

Horchanok¹

2019

TAVM

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Researches of fluctuating asymmetry of the bilateral features of fish were conducted in 2018 on the natural reservoirs (the Samara River within Novomoskovsk and Pavlograd districts of Dnipropetrovsk region, the reservoirs of the Dnipro-Oril Nature Reserve). Insignificant deviations from the bilateral symmetry of external features in living organisms may indicate a decrease in the vitality of certain populations. Using of mass, widespread fish species as the test objects is the best way to detect the levels of fluctuating asymmetry. The fluctuating asymmetry of four bilateral features was estimated within 146 units of the common sunfish Lepomis gibbosus, the stone moroco of Pseudorasbora parva and the round goby Neogobius melanostomus in different habitat conditions, the stability of their life circle was evaluated. The number of rays in the interbranchial septum, the number of branching rays in the pectoral and abdominal fins, the number of scales in the lateral line were analysed. The variability of these features on the left and right sides of the fish was evaluated. The lowering of the natural fish populations vitality because of the influence of negative anthropogenic pressure is currently minor. The stability of fish development by the analysis of bilateral features is noted within normal limits of 1 point (Neogobius melanostomus from the reservoirs of the Dnipro-Oril Nature Reserve, Lepomis gibbosus and Pseudorasbora parva from the middle section of the Samara River) or deviates insignificantly from the statutory indicator in 2 points (Lepomis gibbosus from the lower section of the Samara River). Species of Neogobius melanostomus from the reservoirs of the Dnipro-Oril Nature Reserve show the lowest quantitative indices of the bilateral features asymmetry (0.17 of asymmetric detections per feature). This may demonstrate the absence of negative anthropogenic impact on ground fish within the particularly guarded water areas. The application of the analysis of the fluctuating fish asymmetry is the simplest and most accessible way of rapid testing of the water environment state, but complex laboratory studies, including molecular and biochemical ones, must be used to obtain a more accurate and reliable estimate.

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Section: Discussionmentioning

confidence: 99%

Fluctuating fish asymmetry in natural and artificial reservoirs of Dnipro region on example of invasion types

Horchanok¹

2019

TAVM

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“…Результаты полученные в культуре астроцитов и моделях in vivo дополняют известные данные о роли оксидативного стресса в первичных повреждениях митохондрий и последующем нарушении энергетического метаболизма (Fiskum et al, 2008). Генерация оксидативного стресса и нарушения цитоскелета астроцитов показаны в мозге солнечного окуня как результат хронического действия ионов алюминия, который является одним из самых распространенных токсикантов (Novitsky et al, 2013).…”

Section: Discussionunclassified

“…Окислительные повреждения биомолекул, в первую очередьпероксидное окисление мембранных липидов, оксидация белков, повреждение ДНК, являются наиболее значимыми причинами клеточной и функциональной патологии, и, как следствие, снижения жизнеспособности организмов. Поэтому изучение и выявление молеку-лярных маркеров клеточных и функциональных нарушений водных организмов, обитающих в условиях техногенного загрязнения, является одним из приоритетных направлений исследований биологии гидробионтов (Novitsky et al, 2013).…”

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Biomarkers of metabolism disturbance in bivalve molluscs induced by environmental pollution with processed by-products of oil

Sukharenko¹,

Nedzvetsky²,

Kyrychenko³

2017

Biosys. divers.

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Processed by-products of oil are the most common pollutants in all river and sea water. The increase in oxidative stress in bivalve molluscs was studied in both tissues of the hepatopancreas and the gill. The model for artificial treatment with processed by-products of oil was performed in a laboratory experiment with the river mollusc Dreissena polymorpha Pallas, 1771. The exposure of the molluscs over 28 days to mazut 50 mg/l induced significant increase of both final product of lipid peroxidation (LPO) and antioxidant enzime activity. A significant increase in LPO was observed in the hepatopancreas and gill of D. polymorpha treated with mazut compared to the control group. Antioxidant enzyme activity of cartalase, supeoxide dismutase, glutatione reductase and glutatione-S-transferase showed a greater increase (by almost 1.5 times) in the hepatopancreas than in the gill of D. polymorpha. A similar LPO growth and modulation of antioxidant enzyme activity were determined in the hepatopancreas and gill of the mussel Mytilus galloprovincialis Lamarck, 1879 collected in an area polluted with resins, hydrocarbons and asphaltenes, Donuzlav lake in the Kerch gulf. Varied cellular reactivation of the antioxidant enzyme system in the hepatopancreas rather than the gill was observed in both kinds of mollusc Dreissena and Mytilus. The obtained results are evidence of the higher sensitivity of the hepatopancreas cells of bivalve molluscs to organic pollutants compared to the gill cells.

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“…The detrimental effect of environmental toxicity was demonstrated in the brain of fish exposed to a complex of urban and refinery waste pollution caused by a tanker incident (Sukharenko et al, 2008). Previous results have shown growing evidence of the successful application of neurospecific proteins for estimation of the harmful effect of heavy metal, aluminium ions and industrial pollutant mixture (Nedzvetsky et al, 2011;Novitsky et al, 2013;Sukharenko et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…The cytoskeleton proteins, reparative protein A (RPA70), transcriptional regulator nuclear factor kappa-B cells (NF-kB) and DNA damage repairing poly-(ADP-ribose)-polymerase (PARP) were reported as valid biomarkers to estimate aluminium, cadmium, mercury and copper neurotoxicity (Novitsky et al, 2013;Nedzvetsky et al, 2018;Kirici et al, 2019). The study of the effect environmental toxicity on the brain is one of the relevant and major areas of research (Legradi et al, 2018;Pereira et al, 2019;Taysi et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Local industrial pollution induces astrocyte cytoskeleton rearrangement in the dice snake brain: GFAP as a biomarker

et al. 2020

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The present study was designed to evaluate the responsiveness of modulation of glial fibrillary acidic protein (GFAP) content and its fragmentation in the snake brain as a biomarker of local industrial pollution of aquatic ecosystems. Despite GFAP being a well known cytoskeleton marker of astrocytes’ reactivity in the brain of vertebrates, its expression in the snake brain remains insufficiently described. The GFAP expression and its fragmentation were detected using the immunoblot method in the snake brain. ROS level was determined with dichlorofluorescein diacetate fluorescence. The content of the glial fibrillary acidic protein (GFAP) of filament (cytoskeleton) and soluble (cytosol) fractions in the brain of dice snake Natrix tessellata from three ecosystems with different rates of industrial pollution were studied (two polluted and one clean control site). Characteristic increase in GFAP fragmentation was noted for the snakes from both the researched polluted sites. Significant increase in the content of the GFAP cleaved polypeptide fragments induced by industrial pollution exposure was confirmed in the snakes’ brains. Meaningful GFAP fragmentation was determined in snake brain astrocytes as an increase in cleaved fragments of 47–35 kDa molecular weight for both soluble and cytoskeletal GFAP fractions. We found significant abnormality in the ratio of the GFAP soluble fraction to the cytoskeletal one in contaminant-exposed dice snakes. It should testify to significant metabolic disturbance in nerve cells of the dice snakes. Furthermore, growth of reactive oxygen species level as the main cause of oxidative stress was determined in brains of the snakes exposed to environmental toxicity. Thus, astrocyte cytoskeleton disorders are associated with pollutant-induced redox imbalance in the snake brain. Despite the limited data on glial cell biology in the reptilian brain, the observed results prove that snake astrocytes can respond to the environmental toxicity using typical astroglial response. The presented results evidence that monitoring of molecular characteristics of glial cytoskeleton in dice snakes could be used as reliable biomarker of neurotoxicity and adverse effects of industrial pollution. Further studies are required to elucidate the role of astrocyte cytoskeleton in the response against neurotoxic contaminants.

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Molecular Biomarkers of Al3+ Effects on Induction of Oxidative Stress and Cellular Reactivation in Organism of Lepomis gibbosus (Pisces: Centrarchidae)

Cited by 4 publications

References 0 publications

Fluctuating fish asymmetry in natural and artificial reservoirs of Dnipro region on example of invasion types

Fluctuating fish asymmetry in natural and artificial reservoirs of Dnipro region on example of invasion types

Biomarkers of metabolism disturbance in bivalve molluscs induced by environmental pollution with processed by-products of oil

Local industrial pollution induces astrocyte cytoskeleton rearrangement in the dice snake brain: GFAP as a biomarker

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