Molecular biology test for rapid detection cagAgen EPIYA motif in H.pylori isolates v1

Abstract

In this study , we aimed to determine the current circulation of cagA gene EPIYA motifs present in Colombian Helicobacter pylori isolates using a rapid molecular test. The cagA gene 3' region was amplified through conventional Polymerase Chain Reaction (PCR) and PCR products obtained were sequenced and analyzed with bioinformatics tools.Aditionally, to confirm the prediction of the number EPIYA C repeats based on the PCR product molecular weight, reamplification and secuencing analysis were performed.