Molecular Biology of Thyroid Cancer

Viglietto, Giuseppe; Marco, Carmela De

doi:10.5772/18732

Cited by 3 publications

(5 citation statements)

References 133 publications

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“…Taken together, our data indicated different potential for anoikis resistance and AIG capacity among the three thyroid cancer cell lines, in ascending order for BCPAP (papillary type), FTC133 (follicular type), and ARO (anaplastic type) cell lines, consistent with their metastatic potential as observed in clinical pathology (8). Additionally, our results supported the use of the ARO cell line as a suitable model for further studies into the mechanisms of anoikis resistance and AIG in metastatic thyroid cancer.…”

Section: Resultssupporting

confidence: 77%

“…Indeed, primary thyroid cancer is completely curable by available treatments whereas metastatic thyroid cancer is incurable, leading to death and significantly reducing the survival rate to approximately 50% within 5 years (5, 7). More than 90% of all thyroid cancer types are derived from thyroid epithelial cells or follicular cells that are clinically classified into three subtypes based on their pathology, namely papillary thyroid carcinoma (PTC), follicular thyroid carcinoma (FTC) and anaplastic thyroid carcinoma (ATC) (8). Clinical information indicates a metastatic potential in increasing order for PTC, FTC and ATC.…”

mentioning

confidence: 99%

“…Clinical information indicates a metastatic potential in increasing order for PTC, FTC and ATC. Approximately 15-50% of PTCs invade neighboring tissues and metastasizes only to regional lymph nodes, about 20% of FTCs metastasize to distant organs including bone and lung, while ATC is the most aggressive type, with more than 98% of all cases metastasizing to bone, lung and brain (8,9). The difference in their metastatic property makes thyroid cancer a good model for studying metastasis.…”

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confidence: 99%

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Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells

Saharat

Lirdprapamongkol

Chokchaichamnankit

et al. 2018

Cancer Genomics Proteomics

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Background/Aim: Resistance to anoikis is a prerequisite step in metastasis, a major cause of death in patients with cancer, including thyroid cancer. Impairing anoikis resistance is a possible strategy for therapy of metastatic cancer. We, therefore, we aimed to investigate the key players of anoikis resistance. Materials and Methods: Papillary-type (BCPAP), follicular-type (FTC133), and anaplastic-type (ARO) thyroid carcinoma cells, cultured in poly(2-hydroxyethyl methacrylate)-coated plates to mimic circulating cells, were used as model systems in this study. Flow cytometry and softagar assays were used to determine cells exhibiting anoikis resistance. Proteomics was used to identify candidate proteins and validated using western blot and siRNA knockdown. Results: Only ARO cells showed both anoikis resistance potential and anchorage-independent growth ability. Tumor susceptibility gene 101 protein (TSG101) was identified to be potentially important in anoikis resistance, which was confirmed by an increase in anoikis and expression of a proapoptotic protein (BCL-2 like protein 4) and an apoptotic marker (cleaved poly-ADP ribose polymerase) in floating siTSG101-knockdown cells. Conclusion: To our knowledge, this is the first study that implicates the importance of TSG101 in anoikis resistance of thyroid cancer.

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Section: Resultssupporting

confidence: 77%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells

Saharat

Lirdprapamongkol

Chokchaichamnankit

et al. 2018

Cancer Genomics Proteomics

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“…The differential diagnosis of well-differentiated thyroid carcinomas such as papillary thyroid carcinoma and follicular thyroid carcinoma requires skilled pathology examination from fine-needle aspiration of the thyroid nodule. Even though both types of carcinomas are treatable by thyroidectomy and iodine ablation, follicular thyroid carcinoma is considered to be more aggressive and has higher recurrence than papillary thyroid carcinomas (4,(22)(23)(24). Treatment regimens using molecular-targeted chemotherapy also differ, so it is prudent to distinguish between papillary thyroid carcinoma and follicular thyroid carcinoma (25).…”

Section: Resultsmentioning

confidence: 99%

Unveiling a novel biomarker panel for diagnosis and classification of well-differentiated thyroid carcinomas

et al. 2016

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Thyroid cancer is the most common human endocrine malignancy with increasing global incidence. Papillary thyroid carcinomas (PTC) and follicular thyroid carcinomas (FTC) are well-differentiated thyroid cancers (WDTC) accounting for 95% of all thyroid cancer cases, with survival rates of almost 100% when diagnosed early. Since PTC and FTC have different modes of metastasis, they require different treatment strategies. Standard diagnosis by fine needle aspiration with cytopathological examination can be inaccurate in approximately 10-30% of all cases and difficult to definitively classify as WDTC. Currently, there is no single or panel of biomarkers available for thyroid cancer diagnosis and classification. This study identified novel biomarkers for thyroid cancer diagnosis and classification using proteomics, which may be translated into a biomarker panel for clinical application. Two-dimensional SDS-PAGE and mass spectrometry were used to identify potential biomarkers in papillary and follicular thyroid carcinoma cell lines, and the biomarkers were validated in five PTC and five FTC tissues, with their adjacent normal tissues from Thai patients. Eight biomarkers could distinguish PTC from normal tissues, namely enolase 1, triose phosphate isomerase, cathepsin D, annexin A2, cofilin 1, proliferating cell nuclear antigen (PCNA), copine 1 and heat shock protein 27 kDa (HSP27). These biomarkers can also discriminate FTC from normal tissues, except for annexin A2. On the contrary, annexin A2, cofilin 1, PCNA and HSP27 can be used to classify the types of WDTC. These findings have potential for use as a novel multi-marker panel for more accurate diagnosis and classification to better guide physicians on thyroid cancer treatment. Moreover, our results suggest the involvement of proteins in cell growth and proliferation, and the p53 pathway in the carcinogenesis of WDTC, which may lead to targeted therapy for thyroid cancer.

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“…Selain itu peningkatan asupan iodium juga berkaitan dengan frekuensi mutasi BRAF V600E dengan mekanisme yang belum diketahui dan baru dibuktikan melalui beberapa studi epidemiologi. [23,26] …”

Section: Patogenesis Karsinoma Tiroid Dengan Goiter Sebagai Faktor Prunclassified

Goiter Sebagai Faktor Predisposisi Karsinoma Tiroid

Armerinayanti¹

2017

WMJ

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AbstrakGoiter merupakan pembesaran kelenjar tiroid yang dapat berkaitan dengan gangguan primer pada organ tiroid ataupun akibat stimulasi hormonal atau faktor lain terhadap tiroid. Di Indonesia dan di Bali khususnya kasus karsinoma tiroid mengalami peningkatan sejalan dengan peningkatan kasus goiter endemik maupun non endemik. Goiter memiliki faktor risiko sebesar 2,5 kali lipat untuk menimbulkan karsinoma tiroid. Goiter dapat menimbulkan hiperplasia yang bersifat difusa maupun noduler (nodul tunggal dan multipel). Analisis klonal telah membuktikan bahwa hiperplasia merupakan proliferasi yang bersifat poliklonal. Pada tiroid, ditemukan perubahan pola monoklonal pada kelompok nodul yang sebelumnya merupakan nodul hiperplastik. Perubahan poliklonal menjadi monoklonal ini merupakan interaksi antara adanya lesi hiperplastik sebelumnya dengan predisposisi genetik yang selanjutnya menciptakan lingkungan mutagenik, ditandai oleh peningkatan proliferasi sel disertai pembentukan radikal bebas yang memicu adanya mutasi somatiktirosit. Pemberian suplementasi iodium pada kasus goiter endemik, dikatakan berkaitan dengan adanya kandungan iodine radioaktif nantinya justru menimbulkan kerusakan rantai DNA melalui tata ulang RET-TRK. Kata Kunci: goiter, predisposisi, karsinoma tiroid. Abstract [Goitre as Predisposing Factor of Thyroid Carcinomas]. Goitre is an enlargement of thyroid gland which can be associated with primary thyroid disorders or stimulation of the gland by hormones and other factors. In Indonesia and especially in Bali, increase of Thyroid Carcinoma cases had been associated with either endemic or non endemic goiter. Goitre increases 2.5 fold risk of thyroid carcinoma. Goitre may cause diffuse and nodular hyperplasia (either single or multiple nodules). Clonal analysis has been discovered that hyperplasia is considered to be polyclonal proliferation.Monoclonal alterations had also been found in a subset of thyroid nodules that were previously considered hyperplastic. Alterations of polyclonal to monoclonal pattern was considered as interaction between past history of hyperplastic lesion and genetic predisposition which furthermore forming mutagenic environment such as increases of cell proliferation and also free radicals induce-somatic mutation. Iodine supplementation on endemic goiter related with radioactive iodine content which damaging DNA sequences in the form of RET-TRK rearrangement.

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Molecular Biology of Thyroid Cancer

Cited by 3 publications

References 133 publications

Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells

Tumor Susceptibility Gene 101 Mediates Anoikis Resistance of Metastatic Thyroid Cancer Cells

Unveiling a novel biomarker panel for diagnosis and classification of well-differentiated thyroid carcinomas

Goiter Sebagai Faktor Predisposisi Karsinoma Tiroid

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