Molecular biology of Porcine circovirus: analyses of gene expression and viral replication

Mankertz, Annette; Caliskan, R.; Hattermann, Kim; Hillenbrand, Bernd; Kurzendoerfer, Petra; Mueller, Bettina; Schmitt, Cornelia; Steinfeldt, Tobias; Finsterbusch, Tim

doi:10.1016/j.vetmic.2003.10.014

Cited by 171 publications

(114 citation statements)

References 18 publications

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“…Rep binding may mediate the partial unwinding of the origin and thereby may lead to cruciform extrusion and the generation of an ssDNA region, which is recognized and cleaved by Rep and RepЈ. A similar mechanism for the replication initiator of plasmid pT181 has been described previously (17). A putative helicase activity of the PCV Rep protein associated with ATP hydrolysis could lead to a further unwinding of DNA strands as a prerequisite for DNA synthesis.…”

Section: Discussionmentioning

confidence: 61%

Functional Analysis of cis - and trans -Acting Replication Factors of Porcine Circovirus Type 1

Steinfeldt

Finsterbusch

Mankertz

2007

J Virol

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Section: Discussionmentioning

confidence: 61%

Functional Analysis of cis - and trans -Acting Replication Factors of Porcine Circovirus Type 1

Steinfeldt

Finsterbusch

Mankertz

2007

J Virol

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“…In TTSuV1-ORF1, motif III (YxxK) (Huang et al, 2010;Müller et al, 2008;Mushahwar et al, 1999) was located at aa position 14-17, while the same motif was not detected in TTSuV2. However, TTSuV2 contained the RCR motif II (HxQ) (Huang et al, 2010;Mankertz et al, 2004) at the C-terminal region of the ORF1 protein (aa position 613-615). All RCR motifs described were conserved in the TTSuV1 or TTSuV2 genomes analysed ( Supplementary Fig.…”

Section: Sequence Analyses and Prediction Of Ttsuv1 And Ttsuv2 Viral mentioning

confidence: 99%

Expression profile and subcellular localization of Torque teno sus virus proteins

Martínez-Guinó

Ballester

Segalés

et al. 2011

Journal of General Virology

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In the present study, the expression, generation and subcellular localization of Torque teno sus virus (TTSuV) proteins were characterized into two genetically distinct TTSuV species (TTSuV1 and TTSuV2). Following transfection of three TTSuV1 and TTSuV2 full-length ORF (ORF1, ORF2 and ORF3) expression constructs into porcine kidney cells, alternative splice variants encoding new TTSuV protein isoforms were identified for the first time. Proteins encoded from ORF1 and ORF3 were localized in the nucleoli of porcine kidney cells and that of ORF2 in the cytoplasm and nucleus excluding the nucleoli. The subcellular localization of the different protein isoforms was not only similar between distinct TTSuV species but also to the ones described in human Torque teno virus (TTV). Results of the present in vitro study were not based on full-length viral clones but suggested that alternative splicing strategy to generate TTSuV protein isoforms probably occurs in vivo. Obtained data provide new information on molecular biology of TTSuV and anelloviruses, which until now has been solely based on results obtained from human TTV.

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“…17 The replicase and nucleocapsid proteins are produced from the viral open reading frames (ORF) 1 and ORF2, respectively. 13,14 PCV2 has an affinity for cells of monocytic derivation and is thought to require replication of viral DNA within infected cell nuclei. 11,20 Viral antigens are produced within 18 hours of PCV2 entry into the cell.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for the Detection of Porcine Circovirus Type 2 Replicative Double Stranded Viral DNA and Nonreplicative Single Stranded Viral DNA in Tissue Sections

2007

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Abstract. Porcine circovirus type 2 (PCV2), an economically important pathogen of swine, is the necessary cause of post weaning multisystemic wasting disease (PMWS); PCV2 infection is associated with porcine dermatitis and nephritis syndrome (PDNS). Current immunohistochemical (IHC) methodologies identify PCV2 antigens but are not capable of differentiating replicating virus from nonreplicating virion particles in tissue sections. In this paper, a combination of IHC using commercial monoclonal antibodies specific for single stranded (ss) and double stranded (ds) DNA and PCV2 specific in situ hybridization (ISH) was used to show the specificity of the former for PCV2 DNA in tissue sections from PCV2-infected gnotobiotic pigs. Cold-ethanol-fixed tissue sections were superior to formalin-fixed tissues for detection of PCV2 DNA, presumably due to the lack of protein cross-linking in the latter. These data demonstrate that conventional IHC detects PCV2 DNA forms in experimentally infected PCV2-positive gnotobiotic porcine tissue sections that are minimally compromised by either formalin fixation or the hybridization conditions needed for ISH.

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Molecular biology of Porcine circovirus: analyses of gene expression and viral replication

Cited by 171 publications

References 18 publications

Functional Analysis of cis - and trans -Acting Replication Factors of Porcine Circovirus Type 1

Functional Analysis of cis - and trans -Acting Replication Factors of Porcine Circovirus Type 1

Expression profile and subcellular localization of Torque teno sus virus proteins

A Novel Method for the Detection of Porcine Circovirus Type 2 Replicative Double Stranded Viral DNA and Nonreplicative Single Stranded Viral DNA in Tissue Sections

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