Molecular Biology of Pome Fruit Ripening and Senescence: Concepts, Status, Perspectives and Future Prospects

Dilley, David R.; Wilson, Ian; Kuai, Jianping; Poneleit, Loelle; Zhu, Yanfang; Pekker, Y.

doi:10.17660/actahortic.1993.326.36

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Potential challenges of proteomics in fruit and vegetable research were seen in the identification, for example, of the presence of amino-cyclopropane-carboxylate (ACC) oxidase related to fruit ripening. However, due to technical limitations, only a limited number of proteins were identified in apples and bananas related to their ripening [ 49 , 50 ]. Proteomic approaches, based on 2-DE technology using IGP strips, IEF/SDS-PAGE and mass spectrometry analysis enabled the identification of 22 proteins related to tomato ripening [ 51 ] or, by 2-DE and MALDI/TOF, of some proteins expressed after tomato infection with blossom-end rot [ 52 ].…”

Section: Proteomics-on-a-chip: Promising Development In Food Scienmentioning

confidence: 99%

Protein Analysis-on-Chip Systems in Foodomics

et al. 2012

View full text Add to dashboard Cite

Protein compositional data can address nutritional, packaging, origin/authenticity, processing history, safety and other quality questions. Such data has been time-consuming and expensive to generate until recently but “protein analysis on a chip” systems are now available that can analyze a complex food sample in a few minutes and do not require great protein analytical expertise. We review some of the main new approaches with examples of their application and discuss their advantages and disadvantages.

show abstract

Section: Proteomics-on-a-chip: Promising Development In Food Scienmentioning

confidence: 99%

Protein Analysis-on-Chip Systems in Foodomics

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Early work using 2-DE techniques identified some proteins, such as ACC oxidase related to fruit ripening in apples (Dilley et al, 1993) and some ripening related proteins in banana (Dominguez-Puigjaner et al, 1992) and in stone fruit (Abdi et al, 2002). Due to technical limitations, only a limited number of proteins have been identified and characterized.…”

Section: Proteomic Research On Fruits and Vege-tablesmentioning

confidence: 99%

Application of Proteomic Techniques to Fruits and Vegetables

Song¹,

Braun

2008

View full text Add to dashboard Cite

Proteomics and the technology supporting this science are proving to be invaluable in elucidating the tremendous complexity of biological processes. Among proteomic techniques, two-dimensional electrophoresis (2-DE) has been applied to resolve thousands of proteins and 2-DE has been especially useful for comparative studies between paired samples or populations. Improved protein extraction and purification protocols for recalcitrant fruits and vegetables have resulted in 2-DE techniques appearing in almost all fruit and vegetable proteomic studies currently being published. Numerous proteins involving various metabolic pathways have already been reported for tomato, pepper, strawberry, grape, banana, apple and pear. Significant improvements have been made to both gel and non-gel based proteomic research platforms. Better quantitative analyses and higher throughput proteomic technologies will further improve fruit and vegetable proteomic research and will have a significant impact on future breeding and post-harvest handling technologies to improve nutritional and eating quality.

show abstract

“…Transgenic tomato plants have been developed by genetic engineering in which endopolygalacturonase and ethylene biosynthesis has been attenuated and, as a consequence, fruit ripening is delayed. The evolution of molecular biological techniques and experimentation has heralded a renaissance of opportunities to understand and control fruit ripening (Dilley et al, 1993).…”

Section: Introductionmentioning

confidence: 99%