Molecular Biology of Microbial Hydrogenases

Harnessing Fermentation May Enhance the Performance of Biological Sulfate-Reducing Bioreactors

“…A total of 182 FeFe hydrogenases were identified in the genomes of Bacteroidota, Spirochaetota, and Bacillota alone (Figure ). FeFe hydrogenases are well-studied and most commonly associated with H 2 evolution. , The most commonly encoded were the trimeric group FeFe group A3 hydrogenase, which reversibly bifurcates electrons between H 2 , and ferredoxin and NAD . Genes encoding Rnf complexes were also common in these genomes.…”

Presence and fate of micropollutants during anaerobic digestion of sewage and their implications for the circular economy: A short review

“…As an example, Fe and Ni act as cofactors of hydrogenases participating in the acidogenic and methanogenic hydrogenotrophic stages of CH 4 production [63]. Conversely, excessively high trace metal concentrations may result in AD inhibition [64].…”

Metagenome-Assembled Genome Sequence of Sulfuricurvum sp. Strain IAE1, Isolated from a 4-Chlorophenol-Degrading Consortium

“…BLASTn analysis indicated that strain IAE1’s 16S rRNA gene is closely related to that of Sulfuricurvum kujiense strain DSM 16994 (GenBank accession number NR_074398) and an environmental Sulfuricurvum 16S rRNA gene clone, D2CL (EU498374), with sequence similarities of 97.8% and 99.9%, respectively (10). Strain IAE1’s genome harbors a soxABXYZ gene cluster encoding the subunits of a thiosulfate-oxidizing multienzyme complex (11, 12) and genes encoding the Ni/Fe hydrogenase metallocenter assembly proteins HypABCDEF and the HyaA and HyaB subunits of quinone-reactive Ni/Fe-hydrogenases (11, 13, 14). A BLASTp search found that the gene products mentioned above share more than 71% similarity to their homologs in Sulfuricurvum sp.…”