Molecular biology methods for blood cell antigen genotyping in reference laboratories

Guz, Katarzyna; Orzińska, Agnieszka; Michalewska, Bogumiła; Pelc‐Kłopotowska, Monika; Brojer, Ewa; Łętowska, Magdalena

doi:10.5603/jtm.2019.0010

Cited by 4 publications

(2 citation statements)

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“…If possible, k antigen should be determined in K antigen positive donors and the clinically significant antigens of other group systems (Duffy, Kidd, MNS) should be determined in regular donors especially group O. For rare blood types it is recommended to use molecular biology methods to identify donors without high prevalence antigens [40][41][42][43]. In identified donors, the results should be confirmed by serological methods.…”

Section: Red Blood Cell Immunohematology Tests: Organization and Scopementioning

confidence: 99%

“…In blood donors/recipients with discrepant results of RhD in 79% of cases RHD*01W.1, *01W.2 and *01W.3 were identified as not leading to alloimmunization [67]. Genotyping of RBC antigens has also been used in the diagnostics of antibodies in recently transfused or AIHA patients, alloimmunized patients with antibodies against HFA and in screening compatible donors for such blood recipients [41][42][43]. Currently, a large spectrum of methods [i.e.…”

Section: Molecular Diagnostics Of Red Blood Cell Platelet and Granulo...mentioning

confidence: 99%

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Current status and achievements of Polish transfusion medicine

et al. 2021

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Section: Red Blood Cell Immunohematology Tests: Organization and Scopementioning

confidence: 99%

Section: Molecular Diagnostics Of Red Blood Cell Platelet and Granulo...mentioning

confidence: 99%

Current status and achievements of Polish transfusion medicine

et al. 2021

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When and why is red blood cell genotyping applicable in transfusion medicine: a systematic review of the literature

Akinbolaji

2024

Immunohematology

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This review aims to provide a better understanding of when and why red blood cell (RBC) genotyping is applicable in transfusion medicine. Articles published within the last 8 years in peer-reviewed journals were reviewed in a systematic manner. RBC genotyping has many applications in transfusion medicine including predicting a patient’s antigen profile when serologic methods cannot be used, such as in a recently transfused patient, in the presence of autoantibody, or when serologic reagents are not available. RBC genotyping is used in prenatal care to determine zygosity and guide the administration of Rh immune globulin in pregnant women to prevent hemolytic disease of the fetus and newborn. In donor testing, RBC genotyping is used for resolving ABO/D discrepancies for better donor retention or for identifying donors negative for high-prevalence antigens to increase blood availability and compatibility for patients requiring rare blood. RBC genotyping is helpful to immunohematology reference laboratory staff performing complex antibody workups and is recommended for determining the antigen profiles of patients and prospective donors for accurate matching for C, E, and K in multiply transfused patients. Such testing is also used to determine patients or donors with variant alleles in the Rh blood group system. Information from this testing aides in complex antibody identification as well as sourcing rare allele-matched RBC units. While RBC genotyping is useful in transfusion medicine, there are limitations to its implementation in transfusion services, including test availability, turn-around time, and cost.

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Model research of the pig’s microbiome based on “one health” concept in the light of the shared human and animal health

Satora

Rząsa

Rypuła

et al. 2020

Postepy Hig Med Dosw

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The human microbiome in terms of the number of bacteria exceeds the number of cells in the human body. It is defined as an additional "forgotten organ" and plays a key role in maintaining a high health status, which is conditioned by the maintenance of certain proportions and natural relations between bacteria and cells of the host organism. New diagnostic methods can enable profiling not only the human microbiome, but also livestock. An innovative analytical method, which is next generation sequencing (NGS), is increasingly used in the study of the microbiome. Many bacteria are referred to as "uncultivated" or "non-culturable" and metagenomics has played an important role in detecting these bacteria and has contributed to the development of new media for their cultivation. The main application of NGS in microbiology is to replace the conventional characterization of pathogens based on the assessment of morphology, staining properties and metabolic traits with their genome related characteristics. There are several platforms i.e. "diagnostic tools" that use a variety of DNA sequencing technologies, among others Ion Torrent Personal Genome Machine (PGM), Pacific Biosciences (PacBio) and Illumina MiSeq. In the case of swine microbiome, studies of the microbiome with the use of modern sequencing technologies seem to be particularly interesting in the aspect of the upcoming, inevitable changes in preventive and therapeutic procedures in animals. Analyses of this type enable an in-depth assessment of the impact of specific factors on the population of intestinal microbes and learning how to "form" the composition of the microbiome in order to improve the quality of husbandry and to maintain the proper pig’s health status, integrate with the concept of the shared human and animal health.

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Molecular biology methods for blood cell antigen genotyping in reference laboratories

Cited by 4 publications

References 40 publications

Current status and achievements of Polish transfusion medicine

Current status and achievements of Polish transfusion medicine

When and why is red blood cell genotyping applicable in transfusion medicine: a systematic review of the literature

Model research of the pig’s microbiome based on “one health” concept in the light of the shared human and animal health

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