Molecular biology and regulation of abscisic acid biosynthesis in plants

Liotenberg, Sylviane; North, Helen; Marion‐Poll, Annie

doi:10.1016/s0981-9428(99)80040-0

Cited by 97 publications

(71 citation statements)

References 50 publications

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“…5). This appears to support the theory that ZEP gene expression does not regulate ABA biosynthesis in leaves (Liotenberg et al, 1999), where its expression is more crucial to the control of carotenoid synthesis (Finkelstein and Rock, 2002). In Arabidopsis, three members of the six-member NCED gene family were also induced by infection with the hemibiotrophic bacterial pathogen Pseudomonas syringae, resulting in a significant elevation of ABA level in infected leaves (Fan et al, 2009).…”

Section: Discussionsupporting

confidence: 64%

“…In order to determine whether these stress treatments were activating ABA biosynthesis in the plant tissues, we examined the key rate-limiting steps in the plant ABA biosynthetic pathway catalyzed by ZEP and NCED (Liotenberg et al, 1999). Previous studies on grapevine leaves have showed that water stress specifically induced VvNCED1 but not VvZEP, and this is associated with an increase in bulk leaf ABA concentrations (Soar et al, 2004(Soar et al, , 2006.…”

Section: Discussionmentioning

confidence: 99%

“…As ABA treatment of grapevine leaves was observed to result in changes in transcription of VvHT5, VvHT1, and VvcwINV that were similar to those observed in response to powdery mildew infection and wounding, we examined the effect of these stress treatments on the transcription of zeaxanthin epoxidase (ZEP) and 9-cis-epoxycarotenoid dioxygenase (NCED), which are key steps in the regulation of ABA biosynthesis (Liotenberg et al, 1999). In grapevine, a single VvZEP gene and two VvNCED genes (VvNCED1 and -2) have been identified, but only VvNCED1 transcription appears to be linked to ABA biosynthesis in leaves under stress conditions; VvNCED2 appears to be developmentally regulated (Soar et al, 2004).…”

Section: Biotrophic Pathogen Infection and Wounding Induce The Aba Bimentioning

confidence: 97%

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Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

2010

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Biotrophic fungal and oomycete pathogens alter carbohydrate metabolism in infected host tissues. Symptoms such as elevated soluble carbohydrate concentrations and increased invertase activity suggest that a pathogen-induced carbohydrate sink is established. To identify pathogen-induced regulators of carbohydrate sink strength, quantitative real-time polymerase chain reaction was used to measure transcript levels of invertase and hexose transporter genes in biotrophic pathogen-infected grapevine (Vitis vinifera) leaves. The hexose transporter VvHT5 was highly induced in coordination with the cell wall invertase gene VvcwINV by powdery and downy mildew infection. However, similar responses were also observed in response to wounding, suggesting that this is a generalized response to stress. Analysis of the VvHT5 promoter region indicated the presence of multiple abscisic acid (ABA) response elements, suggesting a role for ABA in the transition from source to sink under stress conditions. ABA treatment of grape leaves was found to reproduce the same gene-specific transcriptional changes as observed under biotic and abiotic stress conditions. Furthermore, the key regulatory ABA biosynthetic gene, VvNCED1, was activated under these same stress conditions. VvHT5 promoter::b-glucuronidase-directed expression in transgenic Arabidopsis (Arabidopsis thaliana) was activated by infection with powdery mildew and by ABA treatment, and the expression was closely associated with vascular tissue adjacent to infected regions. Unlike VvHT1 and VvHT3, which appear to be predominantly involved in hexose transport in developing leaves and berries, VvHT5 appears to have a specific role in enhancing sink strength under stress conditions, and this is controlled through ABA. Our data suggest a central role for ABA in the regulation of VvcwINV and VvHT5 expression during the transition from source to sink in response to infection by biotrophic pathogens.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Biotrophic Pathogen Infection and Wounding Induce The Aba Bimentioning

confidence: 97%

See 1 more Smart Citation

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

2010

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“…The amount of ABA in the tissue is regulated in several metabolic steps, both in the biosynthesis and inactivation steps. ABA is synthesized from phytoene (Figure 3), a carotenoid produced from pyruvate and glyceraldehydes-3-phosphate (Cutler and Krochko, 1999;Liotenberg et al, 1999). In the plastids phytoene is converted to ζ-carotene by phytoene desaturase and then to β-carotene, lycopene and zeaxanthin.…”

Section: Aba Biosynthesis and Metabolismmentioning

confidence: 99%

Stomatal Responses to Drought Stress and Air Humidity

Le¹,

Torre²,

Olsen³

et al. 2011

Abiotic Stress in Plants - Mechanisms and Adaptations

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“…Recently, genes encoding ABA biosynthetic enzymes have been cloned, which has led to a better understanding of the regulation of ABA biosynthesis (2)(3)(4). The most important advances were the isolation of a gene for zeaxanthin epoxidase (ZEP), using the Nicotiana plumbaginifolia mutant aba2 (5) and the isolation of the 9-cis-epoxycarotenoid dioxygenase (NCED) gene, using the maize mutant vp14 (6,7).…”

mentioning

confidence: 99%

The Arabidopsis aldehyde oxidase 3 ( AAO3 ) gene product catalyzes the final step in abscisic acid biosynthesis in leaves

Seo

Peeters

Koiwai

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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385

310

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Abscisic acid (ABA) is a plant hormone involved in seed development and germination and in responses to various environmental stresses. The last step of ABA biosynthesis involves oxidation of abscisic aldehyde, and aldehyde oxidase (EC 1.2.3.1) is thought to catalyze this reaction. An aldehyde oxidase isoform, AO␦, encoded by AAO3, one of four Arabidopsis aldehyde oxidase genes (AAO1, AAO2, AAO3, and AAO4), is the most likely candidate for the enzyme, because it can efficiently catalyze the oxidation of abscisic aldehyde to ABA. Here, we report the isolation and characterization of an ABA-deficient Arabidopsis mutant that maps at the AAO3 locus. The mutant exhibits a wilty phenotype in rosette leaves, but seed dormancy is not affected. ABA levels were significantly reduced in the mutant leaves, explaining the wilty phenotype in rosettes, whereas the level in the mutant seeds was less reduced. No AO␦ activity could be detected in the rosette leaves of the mutant. Sequence data showed that the mutant contains a G to A substitution in the AAO3 gene. The mutation causes incorrect splicing of the ninth intron of AAO3 mRNA. We thus conclude that the ABA-deficient mutant is impaired in the AAO3 gene and that the gene product, AO␦, is an aldehyde oxidase that catalyzes the last step of ABA biosynthesis in Arabidopsis, specifically in rosette leaves. Other aldehyde oxidases may be involved in ABA biosynthesis in other organs.

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Molecular biology and regulation of abscisic acid biosynthesis in plants

Cited by 97 publications

References 50 publications

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

Involvement of Abscisic Acid in the Coordinated Regulation of a Stress-Inducible Hexose Transporter (VvHT5) and a Cell Wall Invertase in Grapevine in Response to Biotrophic Fungal Infection

Stomatal Responses to Drought Stress and Air Humidity

The Arabidopsis aldehyde oxidase 3 ( AAO3 ) gene product catalyzes the final step in abscisic acid biosynthesis in leaves

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