Molecular Biology

Neubauer, Andreas; Thiede, C.; Nagel, Stefan

doi:10.1007/978-3-642-58803-7_3

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…The polymerase chain reaction [45,46] is a method of in vitro amplifi cation of a defi ned DNA target that is fl anked by regions of known sequence. The development of this technique has greatly expanded the diagnostic potential of molecular genetics as a result of its ability to generate almost limitless copies of selected target DNA sequences.…”

Section: The P Olymerase C Hain R Eactionmentioning

confidence: 99%

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Special Techniques Applicable to Bone Marrow Diagnosis

2009

Bone Marrow Pathology

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Section: The P Olymerase C Hain R Eactionmentioning

confidence: 99%

“…In real -time quantitative PCR (RQ -PCR) the PCR technique results in displacement of a fl uorogenic product -specifi c probe, which is degraded during the reaction generating a fl uorescent signal. Nested PCR is a technique that fusion genes and for IGH locus rearrangements [45,53] .…”

Section: Modifi Cations Of Pcr and Rt -Pcr T Echniquesmentioning

confidence: 99%

Special Techniques Applicable to Bone Marrow Diagnosis

2009

Bone Marrow Pathology

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IFN‐α enhances CD40 ligand‐mediated activation of immature monocyte‐derived dendritic cells

Luft¹,

Luetjens²,

Hochrein³

et al. 2002

International Immunology

116

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Type I IFN are immune modulatory cytokines that are secreted during early stages of infection. Type I IFN bridge the innate and the adaptive immune system in humans and mice. We compared the capacity of type I and II IFN to induce the functional maturation of monocyte-derived dendritic cells (MoDC). Extending our earlier observation that type I IFN promote DC maturation, we report that these cytokines also enhance DC differentiation by augmenting CD40 ligand (CD40L)-induced cytokine secretion by MoDC. Type I IFN alone were poor inducers of MoDC maturation as compared with other stimuli. They up-regulated the expression of HLA-DR, CD80, CD86, partially CCR7 but not CD83, partially reduced antigen-uptake function, increased the levels of IL-12p35 mRNA, and prolonged surface expression of peptide-MHC class I complexes for presentation to cytotoxic T lymphocytes, but did not induce migration towards CCL21 chemokine. However, type I IFN were potent co-factors for CD40L-mediated function. Here, they enhanced CD40L-mediated IL-6, IL-10 and IL-12p70 secretion. Furthermore, when combined with IL-1beta and/or IL-4, IFN-alpha2a type I IFN increased CD40L-mediated IL-12p70 production by 2- to 3-fold, and biased the IL-12 p40/p70 ratio towards the IFN-gamma inducing p70 heterodimer, this correlating with higher levels of IFN-gamma secretion by allogeneic T cell subsets and NK cells. Our results suggest that the rapid expression of CD40L, IFN and IL-1beta at sites of infection and inflammation can act in concert on immature DC, thereby linking innate and adaptive immune responses. In this way, type I IFN play a dual role as DC maturation factors and enhancers of CD40L-mediated DC activation.

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Molecular Biology

Cited by 2 publications

References 27 publications

Special Techniques Applicable to Bone Marrow Diagnosis

Special Techniques Applicable to Bone Marrow Diagnosis

IFN‐α enhances CD40 ligand‐mediated activation of immature monocyte‐derived dendritic cells

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