Molecular biological methods of detection and identification of microorganisms in the environment

Kozyrovska, N.; Kovtunovych, Gennadiy

doi:10.7124/bc.0003aa

Cited by 2 publications

(1 citation statement)

References 107 publications

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“…It is known that REP elements (short repetitions of 32 bp) are widely used as targets for the identification of microorganisms. The result of the amplification reaction of the bacterial genome sites between the REP elements is the formation of a set of DNA fragments separated in agarose gel, which reflect the organization of the genome of a particular microorganism (Judd et al, 1993;Kozyrovskaya and Kovtunovich, 1994). For determination of genetic heterogeneity and detection of related groups in strains of soybean nodule bacteria, their genotyping was performed using REP-PCR.…”

Section: Rep-pcr Amplificationmentioning

confidence: 99%

Characterization of Bradyrhizobia Associated With Soybean Plants Grown in Ukraine

Krutylo¹,

Leonova²,

Nadkernychna³

2021

J microb biotech food sci

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The phenotypic and genotypic features of soybean nodule bacteria with different growth rates common in soils of Ukraine have been studied. The obtained results suggest high heterogeneity of soybean microsymbionts. It has been established that all studied strains are able to form active symbiotic relationships with soybean. When interacting with Chinese cowpea, intensive-growing strains form an ineffective symbiosis with a low nitrogen fixation level (phenotype Nod+Fix–), while slow-growing strains form complete nitrogen fixing systems (phenotype Nod+Fix+). Upon cultivation in the mannitol yeast medium, intensive-growing strain B. japonicum KC19 produces a significant amount of auxins and cytokinins, while the slow-growing strain B. japonicum KC23 is significantly superior by the amount of gibberellins. According to the organization of the genome defined in REP-PCR, studied strains of soybean rhizobia belong to two genetic groups, which corresponds to the division of strains into groups by the growth rate (slow- and intensive-growing). Based on the RFLP analysis of rpoB gene, using restriction enzymes MspI, HaeIII and NdeII, intensive-growing strains were combined into one rpoB type, while slow-growing strains belong to 2-3 rpoB types.

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Section: Rep-pcr Amplificationmentioning

confidence: 99%

Characterization of Bradyrhizobia Associated With Soybean Plants Grown in Ukraine

Krutylo¹,

Leonova²,

Nadkernychna³

2021

J microb biotech food sci

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Interaction of endophytic bacteria with the plant on cellular and molecular level

Kozyrovska

1998

Biopolym. Cell

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Взаємодія ендофітних бактерій з рослиною на клітинному та молекулярному рівнях її. О-Козировська Інститут молекулярної біології і генетики НАН України 252143, Київ, вул. Академіка Заболотного, 150 В огляді надається інформація про локалізацію ендофітних бактерій у тканинах рослий, а також обговорюються шляхи, та механізми проникнення бактерій всередину рослини. Розглядаються методи детещїї ендофітів (Середині рослин (цитохімічні, імунологічні, молекулярно-гепетшші).

show abstract

Molecular biological methods of detection and identification of microorganisms in the environment

Cited by 2 publications

References 107 publications

Characterization of Bradyrhizobia Associated With Soybean Plants Grown in Ukraine

Characterization of Bradyrhizobia Associated With Soybean Plants Grown in Ukraine

Interaction of endophytic bacteria with the plant on cellular and molecular level

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