Molecular biological methods in environmental engineering

Abstract

Environmental bioremediation technologies are undoubtedly more economical and environmental friendly, and present a good application prospect. Microorganisms can be used to monitor environmental changes for their quickly respond to environmental changes. Microbiological molecular biology methods have attracted more and more attention from human beings, and their applications in the environment is also expanding. Genomics, transcriptomics, proteomics, and metabolomics are four major molecular biology techniques. This review summarizes application of molecular biological methods in environmental engineering in 2017. The summary includes methods for DNA, RNA, and protein extraction, the application of four major technologies in the environmental field, and commonly used methods for in situ monitoring in the environment, including fluorescence in situ hybridization, real-time quantitative PCR, and amplicons sequencing technology.

Molecular Diagnostic Tools Applied for Assessing Microbial Water Quality

“…For instance, DNA/RNA extraction is a crucial step for downstream molecular analysis, which is equally important for all kinds of DNA/RNA-based methods. Various extraction methods for isolating environmental DNA/RNA (eDNA/eRNA) from diverse matrices (e.g., soil and water) have been well-described and summarized in the published review reports [172][173][174]. Among them, the phenol-chloroform extraction method and a wide variety of commercial purification kits have been broadly employed in numerous aquatic ecosystem studies.…”