Molecular Biological Investigations of Acc Oxidase and Its Expression Attending Apple Fruit Ripening

Dilley, David R.; Kuai, Jianping; Wilson, Ian; Pekker, Y.; Zhu, Yunqing; Burmeister, Douglas M.; Beaudry, Randolph M.

doi:10.17660/actahortic.1995.379.1

Cited by 9 publications

(6 citation statements)

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“…Recombinant apple ACCO1 with a His-Tag bound to a Ni 2+ column was employed as a protein affinity column as ‘bait’ to determine whether any proteins from apple fruit can bind to ACCO through protein–protein interactions ( Dilley et al 1995 ). Products of an in vitro phosphorylation assay conducted with a 100 000 g membrane fraction collected from pre- and postclimacteric apple fruits in the presence or absence of ACCO were passed through the ACCO affinity column and subjected to SDS–PAGE analysis.…”

Section: Discussionmentioning

confidence: 99%

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1-Aminocyclopropane-1-carboxylic acid oxidase reaction mechanism and putative post-translational activities of the ACCO protein

et al. 2013

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ACC oxidase (Malus. domestica ACCO1) catalyzes the final step in the biosynthesis of the plant hormone ethylene. ACCO converts 1-aminocyclopropane-1-carboxylic acid(ACC) to ethylene, cyanide, carbon dioxide and water in the presence of ferrous ion, oxygen, ascorbic acid and bicarbonate. Cyanide, a product of the reaction, activates ACCO. Site-directed mutagenesis investigations revealed binding sites for ACC, bicarbonate and ascorbic acid to include; Arg175, Arg244, Ser246, Lys158, Lys292, Arg299 and Phe300. ACCO may be involved in the ethylene signal transduction pathway not directly linked to the ACCO reaction through post-translational modifications. ACCO is subject to auto-phosphorylaton in vitro and promotes phosphorylation of some apple fruit proteins in a ripening-dependent manner.

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Section: Discussionmentioning

confidence: 99%

“…The small arrowheads in the FP lane of (B) indicate the position of the fusion protein and a proteolytically cleaved product of the FP in lanes 7 and 8. (From Dilley et al 1995 . Acta Horticulturae 379 :25–39, with permission from Acta Horticulturae .)…”

Section: Discussionmentioning

confidence: 99%

1-Aminocyclopropane-1-carboxylic acid oxidase reaction mechanism and putative post-translational activities of the ACCO protein

et al. 2013

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“…An in vitro phosphorylation assay using protein extracts of pre- and post-climacteric apple fruit and an ectopically expressed His-tagged Md ACO1 (MDP0000195885) hinted for the first time that ACO protein-protein interactions could exist (Dilley et al, 1995). It was observed that apple ACO1 could interact with unidentified proteins and resulted in the phosphorylation of these associated proteins (and possibly also ACO1 itself) in both pre-and post-climacteric samples (Dilley et al, 1995). Later Dilley et al also speculated about a possible cysteine protease activity of ACO, unrelated to its role in catalyzing the conversion of ACC to ethylene.…”

Section: The Regulation Of Acomentioning

confidence: 99%

1-Aminocyclopropane-1-Carboxylic Acid Oxidase (ACO): The Enzyme That Makes the Plant Hormone Ethylene

2019

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The volatile plant hormone ethylene regulates many plant developmental processes and stress responses. It is therefore crucial that plants can precisely control their ethylene production levels in space and time. The ethylene biosynthesis pathway consists of two dedicated steps. In a first reaction, S-adenosyl-L-methionine (SAM) is converted into 1-aminocyclopropane-1-carboxylic acid (ACC) by ACC-synthase (ACS). In a second reaction, ACC is converted into ethylene by ACC-oxidase (ACO). Initially, it was postulated that ACS is the rate-limiting enzyme of this pathway, directing many studies to unravel the regulation of ACS protein activity, and stability. However, an increasing amount of evidence has been gathered over the years, which shows that ACO is the rate-limiting step in ethylene production during certain dedicated processes. This implies that also the ACO protein family is subjected to a stringent regulation. In this review, we give an overview about the state-of-the-art regarding ACO evolution, functionality and regulation, with an emphasis on the transcriptional, post-transcriptional, and post-translational control. We also highlight the importance of ACO being a prime target for genetic engineering and precision breeding, in order to control plant ethylene production levels.

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“…An additional attempt to use proteomic approaches to determine optimal harvest time was initiated in apples which are harvested at a pre‐climacteric stage, when ethylene production is extremely low. Dilley found in a gel‐based approach that aminocyclopropane carboxylate‐oxidase, the last enzyme in the pathway of ethylene production, was present before the apples began the climacteric increase in ethylene . An ELISA assay was developed to measure the presence of aminocyclopropane carboxylate‐oxidase, as a determinant for apple harvest.…”

Section: Perspectives On Reduction Of Post‐harvest Losses In Perishablesmentioning

confidence: 99%

Post-harvest proteomics and food security

et al. 2013

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To guarantee sufficient food supply for a growing world population, efforts towards improving crop yield and plant resistance should be complemented with efforts to reduce post-harvest losses. Post-harvest losses are substantial and occur at different stages of the food chain in developed and developing countries. In recent years, a substantially increasing interest can be seen in the application of proteomics to understand post-harvest events. In the near future post-harvest proteomics will be poised to move from fundamental research to aiding the reduction of food losses. Proteomics research can help in reducing food losses through (i) identification and validation of gene products associated to specific quality traits supporting marker-assisted crop improvement programmes, (ii) delivering markers of initial quality that allow optimisation of distribution conditions and prediction of remaining shelf-life for decision support systems and (iii) delivering early detection tools of physiological or pathogen-related post-harvest problems. In this manuscript, recent proteomics studies on post-harvest and stress physiology are reviewed and discussed. Perspectives on future directions of post-harvest proteomics studies aiming to reduce food losses are presented.

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Molecular Biological Investigations of Acc Oxidase and Its Expression Attending Apple Fruit Ripening

Cited by 9 publications

References 0 publications

1-Aminocyclopropane-1-carboxylic acid oxidase reaction mechanism and putative post-translational activities of the ACCO protein

1-Aminocyclopropane-1-carboxylic acid oxidase reaction mechanism and putative post-translational activities of the ACCO protein

1-Aminocyclopropane-1-Carboxylic Acid Oxidase (ACO): The Enzyme That Makes the Plant Hormone Ethylene

Post-harvest proteomics and food security

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