Molecular Bioindicators of Pollution in Fish

Denslow, Nancy D.; Sabo-Attwood, Tara

doi:10.1007/978-94-017-9499-2_40

Cited by 3 publications

(5 citation statements)

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“…Transcriptomics combined with computational pathway analysis could provide some useful information on the key events at the cellular or organ level which facilitates predictive outcome effects in organisms or population (59, 68, 115–117). The Omics approach could characterize the biochemical pathways targeted by the contaminants and the common regulators perturbed by the chemical exposure which can be subsequently applied to ecotoxicology research (66).…”

Section: Discussionmentioning

confidence: 99%

“…Transcriptomics can provide important information on global gene expression signatures of environmental contaminants and understanding of the molecular mechanisms underlying the impact of EDCs on aquatic organisms (29, 59, 63–65). Expression microarrays can be used to identify biological pathways and common regulators targeted by contaminants individually and in mixture [Reviewed in (66)]. A number of mathematical models has been developed to predict apical parameters from transcriptome data, which has shown to be consistent with results produced from traditional methods for chemical risk assessment (67, 68).…”

Section: Introductionmentioning

confidence: 99%

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Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

et al. 2018

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Environmental contaminants are known to impair reproduction, metabolism and development in wild life and humans. To investigate the mechanisms underlying adverse effects of contaminants, fathead minnows were exposed to a number of endocrine disruptive chemicals (EDCs) including Nonylphenol (NP), bisphenol-A (BPA), Di(2-ethylhexyl) phthalate (DEHP), and a mixture of the three chemicals for 21 days, followed by determination of the liver transcriptome by expression microarrays. Pathway analysis revealed a distinct mode of action for the individual chemicals and their mixture. The results showed expression changes in over 980 genes in response to exposure to these EDC contaminants individually and in mixture. Ingenuity Pathway core and toxicity analysis were used to identify the biological processes, pathways and the top regulators affected by these compounds. A number of canonical pathways were significantly altered, including cell cycle & proliferation, lipid metabolism, inflammatory, innate immune response, stress response, and drug metabolism. We identified 18 genes that were expressed in all individual and mixed treatments. Relevant candidate genes identified from expression microarray data were verified using quantitative PCR. We were also able to identify specific genes affected by NP, BPA, and DEHP individually, but were also affected by exposure to the mixture of the contaminants. Overall the results of this study provide novel information on the adverse health impact of contaminants tested based on pathway analysis of transcriptome data. Furthermore, the results identify a number of new biomarkers that can potentially be used for screening environmental contaminants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

et al. 2018

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“…VA L E S I N I E T A L. recruitment, changes in fishing pressure and prey availability (Lorenzen & Enberg, 2002;Selleslagh & Amara, 2015). Molecular responses of estuarine fishes to stressors such as pollutants have been examined by many workers (Elliott et al, 2003;Forrester et al, 2003;Minier & Amara, 2009;Denslow & Sabo-Attwood, 2015), but the ecological significance of these effects is often unknown (Clements, 2000).…”

Section: Introductionmentioning

confidence: 99%

Interdecadal changes in the community, population and individual levels of the fish fauna of an extensively modified estuary

Valesini

Cottingham

Hallett

et al. 2017

Journal of Fish Biology

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This study examined inter-period changes over two to three decades in the fish fauna of an urbanized estuary experiencing rapid population growth and a drying climate (Swan-Canning Estuary, Western Australia). Responses were compared at the fish community level (species composition; 1978-2009 in the shallows and 1993-2009 in deeper waters) and at the population and individual levels of an estuarine indicator species, black bream Acanthopagrus butcheri (biomass-abundance and per capita mass at age, respectively; 1993-2009). All three levels showed distinct shifts from earlier to later periods, but their patterns, sensitivity and breadth differed. Community composition changed markedly in the shallows of the lower-middle estuary between the late 1970s and all later periods and moderately between more disparate periods from 1995 to 2009. Several species trends could be linked to the increasing salinity of the estuary or declining dissolved oxygen levels in its middle-upper reaches. Community changes were, however, small or insignificant in the shallow and deeper waters of the upper estuary and deeper waters of the middle estuary, where environmental perturbations are often most pronounced. This may reflect the resilience of the limited suite of species that typify those reaches and thus their lack of sensitivity in reflecting longer-term change at the coarser level of mean abundance. One such species, the selected indicator, A. butcheri, did, however, show marked temporal changes at both the population and individual levels. Biomass decreased markedly in deeper waters while increasing in the shallows from earlier to later periods, presumably reflecting an onshore movement of fish, and per capita body mass in the 2+, 3+ and 4+ year classes fell steadily over time. Such changes probably indicate deteriorating habitat quality in the deeper waters. The study outcomes provide support for a multifaceted approach to the biomonitoring of estuaries using fishes and highlight the need for complementary monitoring of relevant stressors to better disentangle cause-effect pathways.

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“…At the molecular level, there is considerable evidence that contaminants found in MWWTP effluents can alter the expression of genes in tissues of the hypothalamus-pituitary-gonadal axis as well as those transcripts related to sexual differentiation and reproduction [13,19]. Transcriptomics has been used to study the effects of MWWTP effluents on fish molecular responses [21]. Hence, toxicogenomic approaches can broaden our understanding of mechanisms of action of individual chemicals or chemical mixtures in an organism, such as those encountered in freshwater ecosystems exposed to MWWTP effluents.…”

Section: Introductionmentioning

confidence: 99%

“…The oligonucleotide microarray is a tool that can provide insights into transcriptome patterns, allowing one to examine thousands of genes in unison. Transcriptomics has been used to study the effects of MWWTP effluents on fish molecular responses [21]. However, only a small subset of these studies have examined molecular responses in wild fish populations residing downstream of MWWTP discharges [5,[22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Marjan

Martyniuk

Fuzzen

et al. 2017

Enviro Toxic and Chemistry

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The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1223) remained comparable to that downstream of Waterloo (767-3867). Noteworthy was that year and the interaction of year and site explained variability in more than twice the number of transcripts than site alone, suggesting that year and the interaction of year and site had a greater effect on the transcriptome than site alone. Gene set enrichment analysis revealed a gradual reduction in the number of gene ontologies over time at exposure sites, which corresponded with lower contaminant load. Subnetwork enrichment analysis revealed that there were noticeable shifts in the cell pathways differently expressed in the liver preupgrade and postupgrade. The dominant pathways altered preupgrade were related to genetic modifications and cell division, whereas postupgrade they were associated with the immune system, reproduction, and biochemical responses. Molecular pathways were dynamic over time, and following the upgrades, there was little evidence that gene expression profiles in fish collected from high-impact sites postupgrade were more similar to those in fish collected from reference site. Environ Toxicol Chem 2017;36:2108-2122. © 2017 SETAC.

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Molecular Bioindicators of Pollution in Fish

Cited by 3 publications

References 174 publications

Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

Differential Hepatic Gene Expression Profile of Male Fathead Minnows Exposed to Daily Varying Dose of Environmental Contaminants Individually and in Mixture

Interdecadal changes in the community, population and individual levels of the fish fauna of an extensively modified estuary

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

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