Molecular, biochemical and histopathological studies on the ameliorative effect of vitamin C on the renal and muscle tissues of Nile tilapia fish ( Oreochromis niloticus ) affected by the usage of engine oil

Abstract

The present study was performed to evaluate the effect of vitamin C on the performance of Nile tilapia (Oreochromis niloticus) affected by the usage of engine oil. A total of 120 tilapia fish were randomly divided into four equal groups according to dietary treatments applied for 3 weeks: group I (control); group II was exposed to engine oil; group III was exposed to engine oil and supplemented with vitamin C: and group IV was fed only a diet supplemented with vitamin C. According to our results, the group exposed to engine oil (II) showed an induced elevation of kidney function parameters, an upregulation of CYP1A1 mRNA expression level, a reduction in antioxidant status (SOD, GSH) and severe abnormal histological and ultra‐structural changes in the head kidney, trunk kidney and muscle tissue. Supplementation of group III with vitamin C ameliorated the toxic effect induced by engine oil through improvement of kidney function parameters, elevation of the antioxidant status of tissue and improvement of the histological images of renal and muscle tissues. In conclusion, vitamin C has the ability to ameliorate engine oil oxidative stress toxicity in Nile tilapia.

Prefeeding of Clarias gariepinus with Spirulina platensis counteracts petroleum hydrocarbons-induced hepato- and nephrotoxicity

“…The raising in creatinine levels of the benzene group is in the same line as that observed in juvenile Oreochromis niloticus after exposure to water-soluble fractions of crude oil 47 . These observations were supported by renal histo-architecture deteriorations in the present and previous studies 25 , and indicate the ability of xenobiotics to impair the role of the kidney in getting rid of the metabolites by reducing glomerular membrane filterability 48 .…”

“…Benzene and Toluene increased lipid peroxidation derivatives and decreased enzymatic antioxidant activities in the liver of juvenile stages of Clarias gariepinus 6 . A marked change was observed in the glutathione redox system and superoxide dismutase in the liver and kidney of Astyanax altiparanae when challenged with a complex mixture of monocyclic aromatic hydrocarbons 24 Engine oil compromised the cyto-biological features in the kidney of Oreochromis niloticus by depleting reduced glutathione and superoxide dismutase leading to the accumulation of proteinoid metabolic waste products along with adverse modifications on the characteristics of the nephron, melanomacrophage center, and microvasculature 25 .…”

Nano vitamin E improved the antioxidant capacity of broiler chickens

“…T-AOC is an important index of the antioxidant defense system, reflecting the ability of the non-enzymatic antioxidant defense system. Therefore, SOD, GSH-Px, and T-AOC are often used as effective indicators to objectively reflect the antioxidant status in animals ( Zhao et al., 2016 ; Mohamed et al., 2019 ). In the present study, dietary supplementation with NVE increased serum SOD activity and GSH-Px content compared with RVE.…”