Molecular beacon–quantum dot–Au nanoparticle hybrid nanoprobes for visualizing virus replication in living cells

Yeh, Hsiao-Yun; Yates, Marylynn V.; Mulchandani, Ashok; Chen, Wilfred

doi:10.1039/c001553a

Cited by 71 publications

(51 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Later, this group observed that the coverage of MBs can be controlled by varying the MB concentration, by varying the buffer ionic strength, and by addition of short hydroxyterminated alkanethiol diluent molecules during probe [16,33,58,60,65,66]; b MB containing a gold nanoparticle as a quencher (no stem) [61]; c MB containing a silver nanoparticle as a quencher [67]; d MBs containing a nanobarcode particle as a quencher [68][69][70][71][72][73]; e MB containing a single-walled carbon nanotube as a quencher [77]; f MB containing an organic dye as a PLS and GOs as a quencher [76,78,80]; gMB containing a QD as a PLS and GO as a quencher [23,79] assembly onto the NBC particle surface. The optimal coverage for the detection of a target sequence is about 10 12 molecules per square centimeter.…”

Section: Intramolecular Quenchers (Nanoparticles and Nanowires)mentioning

confidence: 97%

“…With improved quenchers, Yeh et al [33] described a hybrid photoluminescent nanoprobe composed of a nuclease-resistant MB backbone, a CdSe-ZnS core-shell QD as the donor, and a gold nanoparticle (AuNP) as the quencher (Fig. 3b).…”

Section: Quantum Dotsmentioning

confidence: 98%

“…The combination of CdS:Mn nanocrystals and AuNPs in a MB scaffold allows selective target detection. As described in "Modification of the photoluminescent species," Yeh et al [33] designed a hybrid photoluminescent probe based on CdSe-ZnS coreshell QDs as donors and AuNPs as quenchers (Fig. 3b), and which was exploited for sensitive and real-time detection of infectious viruses as well as real-time visualization of cell-tocell virus spreading.…”

Section: Intramolecular Quenchers (Nanoparticles and Nanowires)mentioning

confidence: 99%

“…When an excited-state molecule is brought close to a [29][30][31][32]40]; b MB containing a QD as a PLS and a gold nanoparticle as a quencher [33]; c MB containing a QD and an organic dye to form a dual-emission MB [34]; dMB containing a QD with a silica shell and an organic dye for a more stable dual-emission MB [35]; e MB containing a QD as a PLS and graphene oxide (GO) as a quencher [23] molecule in the ground state, an excimer is formed whose emission is redshifted from the emission of the monomer [43]. To date, pyrene has been employed as a PLS to signal the presence of small molecules [44], nucleic acids [45], and proteins [46].…”

Section: Pyrene Excimermentioning

confidence: 99%

See 3 more Smart Citations

Recent trends in molecular beacon design and applications

Huang

Martí

2011

Anal Bioanal Chem

View full text Add to dashboard Cite

A molecular beacon (MB) is a hairpin-structured oligonucleotide probe containing a photoluminescent species (PLS) and a quencher at different ends of the strand. In a recognition and detection process, the hybridization of MBs with target DNA sequences restores the strong photoluminescence, which is quenched before hybridization. Making better MBs involves reducing the background photoluminescence and increasing the brightness of the PLS, which therefore involves the development of new PLS and quenchers, as well as innovative PLS-quencher systems. Heavy-metal complexes, nanocrystals, pyrene compounds, and other materials with excellent photophysical properties have been applied as PLS of MBs. Nanoparticles, nanowires, graphene, metal films, and many other media have also been introduced to quench photoluminescence. On the basis of their high specificity, selectivity, and sensitivity, MBs are developed as a general platform for sensing, producing, and carrying molecules other than oligonucleotides.

show abstract

Section: Intramolecular Quenchers (Nanoparticles and Nanowires)mentioning

confidence: 97%

Section: Quantum Dotsmentioning

confidence: 98%

Section: Intramolecular Quenchers (Nanoparticles and Nanowires)mentioning

confidence: 99%

Section: Pyrene Excimermentioning

confidence: 99%

See 2 more Smart Citations

Recent trends in molecular beacon design and applications

Huang

Martí

2011

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…In addition, Au NPs have been tested as quenchers for semiconductor QDs (see Section 6.5.5). QD-Au NP systems have been used as probes to monitor real-time intracellular gene expression [476], to detect DNA hybridization events [477][478][479][480], and for TNT detection [481]. Polystyrene microspheres surface modified with Au NPs and QDs have been proposed as suitable FRET-probes for bioassays, including measuring protease activity [482].…”

Section: 53mentioning

confidence: 99%

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Sapsford

Wildt

Mariani

et al. 2013

FRET – Förster Resonance Energy Transfer

View full text Add to dashboard Cite

The intrinsic sensitivity (r 6 dependence) of F€ orster (or fluorescence) resonance energy transfer (FRET) to nanoscale changes in the donor/acceptor separation distance has made FRET an invaluable biophysical tool in a variety of applications, ranging from studying the structure and conformation of proteins and nucleic acids to examining biomolecular interactions, including its use in in vitro and in vivo bioassays [1][2][3][4][5][6][7]. While the myriad of FRET configurations and techniques currently in use are covered throughout this book, here we focus primarily on the materials utilized as donor or acceptor probes in FRET rather than the process itself [3,5,8,9]. Our 2006 review paper on this topic serves as the foundation for this updated chapter [3]. The materials were divided into three main categories: organic materials that include "traditional" dye fluorophores, dark quenchers, polymers, and carbon nanomaterials (NMs); inorganic materials such as metal chelates, metal, and semiconductor nanocrystals; and fluorophores of biological origin such as fluorescent proteins (FPs), amino acids, and fluorescence generated from enzymatic bioluminescence (BL) and chemiluminescence (CL). These materials may function as FRET donors and/or acceptors, depending upon experimental design. Many of the new materials developed and/or new donor-acceptor probe combinations used address some of the inherent complications of more traditional FRET materials, including photobleaching, spectral cross talk, and direct excitation of the acceptor species, and examples of these will be highlighted and discussed throughout the chapter. Since the vast majority of FRET applications are biological in nature, they routinely involve some type of biomolecule labeling strategy, which ultimately plays a significant and fundamental role in the success and interpretation of the resulting FRET. Therefore, we begin the chapter with a brief discussion of the bioconjugation techniques commonly utilized for FRET and points to consider, followed by sections highlighting the current and emerging materials that are used, or have the potential to be used, in FRET applications.

show abstract

Molecular Devices: Energy Transfer

Bonacchi

Genovese

Juris

et al. 2012

Supramolecular Chemistry

View full text Add to dashboard Cite

Devices are systems able to perform specific functions, which result from the coordinated action of different components. The design of these systems is, hence, based on the identification or the development of the necessary parts, and in their integration in a suitable supramolecular architecture. The main challenge in such a process is undoubtedly synthetic: the control of the mutual interactions between the active moieties, in fact, requires a fine‐tuning of the distance and orientation of the components and, in the case of physically connected donor‐acceptor moieties, of the electronic properties of the interconnecting linkers. Organization through covalent bonding has, in this context, several advantages, and many examples of covalent supramolecular devices have been reported in the last two decades. Besides, the covalent approach led to the design of fascinating and efficient prototypes, that often require the preparation of beautiful but complicated chemical systems, through time‐demanding multistep synthetic paths and that are not suitable for large‐scale production. Many examples of devices derived from a more traditional supramolecular approach based on self‐assembly and host‐guest interactions have also been reported. In this chapter, some relevant examples of both covalent and self‐organizing supramolecular devices based on energy‐transfer (ET) processes are reviewed. The basic principles of ET are also discussed. Recently, the advent of nanomaterials offered to supramolecular chemists new active components and structural platforms suitable for the design of nanometric molecular devices. At the present stage, nanomaterials represent, in fact, an ideal platform to achieve a reasonable degree of spatial organization especially when the integration of a large number of components is required or desired. This approach allows the achievement of complex, extensive devices otherwise not accessible. Moreover, some nanomaterials have unique photophysical properties and can become themselves active nanosized component of the final device. In this chapter, we discuss selected examples of ET‐based supramolecular devices with increasing complexity and size; going from dyads to multicomponent nanostructures.

show abstract

Molecular beacon–quantum dot–Au nanoparticle hybrid nanoprobes for visualizing virus replication in living cells

Cited by 71 publications

References 28 publications

Recent trends in molecular beacon design and applications

Recent trends in molecular beacon design and applications

Materials for FRET Analysis: Beyond Traditional Dye–Dye Combinations

Molecular Devices: Energy Transfer

Contact Info

Product

Resources

About