Molecular beacon-functionalized gold nanoparticles as probes in dry-reagent strip biosensor for DNA analysis

Mao, Xinhua; Xu, Hui; Zeng, Qingxiang; Zeng, Lingwen; Liu, Guodong

doi:10.1039/b822582f

Cited by 85 publications

(56 citation statements)

References 17 publications

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“…On the basis of this equation, the LOD was determined to be ∼0.24 pg/mL (Cho et al, 2006;Zhao et al, 2014). This value is at least 1000 times more sensitive than the values that were previously reported by the color intensity approach Mao et al, 2009b) or by the fluorescent detection method (Guo et al, 2014). The improvement of the detection sensitivity was attributed to the significant enhancement provided by the DNAconjugated AuNPs.…”

Section: Analytical Performance Of the Sers-based Lateral Flow Biosensormentioning

confidence: 70%

“…LF strips are considered to be one of the most promising techniques due to their simplicity, rapid analysis, relatively low interference due to chromatographic separation, low costs, long-term stability over a wide range of climates, and their ability to be easily used with no requirements for skilled technicians (Chen et al, 2013;Mao et al, 2009b;Shen et al, 2013). Until now, several different types of LF strip have been developed to detect nucleic acids including DNA or miRNA (Fang et al, 2014;Gao et al, 2014;He et al, 2010;Hu et al, 2013;Mao et al, 2009b). For example, a dry-reagent strip biosensor based on a molecular beaconfunctionalized gold nanoparticle probe has been developed for rapid and quantitative detection of specific nucleic acids (Mao et al, 2009b).…”

Section: Introductionmentioning

confidence: 99%

“…Until now, several different types of LF strip have been developed to detect nucleic acids including DNA or miRNA (Fang et al, 2014;Gao et al, 2014;He et al, 2010;Hu et al, 2013;Mao et al, 2009b). For example, a dry-reagent strip biosensor based on a molecular beaconfunctionalized gold nanoparticle probe has been developed for rapid and quantitative detection of specific nucleic acids (Mao et al, 2009b). A simple one-step signal amplification strategy, using oligonucleotide-linked gold nanoparticle aggregates, has also been developed to enhance the sensitivity of the nucleic acids LF assay .…”

Section: Introductionmentioning

confidence: 99%

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A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

Cheng

et al. 2016

Biosensors and Bioelectronics

325

173

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a b s t r a c tUser-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity.

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Section: Analytical Performance Of the Sers-based Lateral Flow Biosensormentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

Cheng

et al. 2016

Biosensors and Bioelectronics

325

173

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Section: Therapeutic Applicationsmentioning

confidence: 91%

“…The technology was reported as superior to antibody-based assay and could analyze thrombin with a detection limit of 0.6 pmol. It was also marked that other proteins such as human serum albumin, IgG and IgM were not interfering in analysis demonstrating the specificity of the developed kit [30].Label-free bioelectronic strategy for measuring target proteins up to femtomolar (fM) concentrations is reported. The invented method is based on ultrasensitive electrochemical measurement of guanine-rich secondary aptamer with or without amplification using polymerase chain reaction.…”

mentioning

confidence: 89%

Untitled

2017

JNMR

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The aptamer is an oligonucleotide which is a short version of biological nucleic acids (such as DNA and RNA) with defined sequence of nucleotides. Based on the complexity of molecule, the aptamers lies in-between protein molecules and small chemical molecules. Aptamers have high specificity and affinity towards target proteins. They are screened from random sequences of oligonucleotides based on the highest affinity for target proteins using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment). Researchers have discovered various applications of aptamers that are ready to replace the therapeutic use of biological proteins (such as antibodies) that have complexity in manufacturing and characterization. The present review describes the structural modification in aptamers such as PEGylation, substitution of functional groups, use of an enantiomeric oligonucleotide and its applications. Aptamers can be deactivated, when needed, by the use of reversal agent that contains oligonucleotide sequence complementary to aptamers. This property of aptamers makes them potential therapeutic from a safety point of view. In recent scenario, aptamers are developed for targeted drug delivery systems by conjugating with drug molecules or delivery vesicles such as liposomes. ' Anti-sense Aptamers' are being developed to silent the expression of genes responsible for the proliferative growth of tissue in cancer. Other applications of aptamers such as environmental monitoring and laboratory testing are also described in this review. Keywords: IntroductionBiotechnology based products are incredibly capturing market shares day by day. Chemistry based synthesis has a limitation when we talk about the tertiary and quaternary structure of large molecules such as protein. The human body is functioning by different biochemical reactions. Protein molecules are broadly involved in every aspect of the biological reaction. In the current paradigm, such functional proteins get expressed in microorganism by genetic variation as a part of the upstream process. Subsequently, these proteins are separated from the microorganisms and purified (as a downstream process) retaining its biological activity to inject in the human body. These complex proteins are difficult to cultivate, separate, characterize and reproduce [1,2].The basic mechanism of protein synthesis in any of the living organism involves transcription and translation of polynucleotide chain of Adenine, Guanine, Thymine, and Cytosine [3]. The sequence of the oligonucleotide is responsible for translating specific protein using a specific sequence of amino acid involved in the synthesis of protein. Currently marketed protein formulations readily provide biological action at the target site when injected into the body. Such protein molecules are produced outside of the human body and injected in purified and active form. However, the stability of proteins is the major concern in the development of protein formulations. Such biological molecules have a tertiary and qua...

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Amplified Detection of a Unique Genomic Viral Marker Using Fluorescently Labeled Liposomes

Shaheen

Fakih

Kobeissi

et al. 2018

Adv Materials Inter

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Sensitive and enzyme‐free detection of specific oligonucleotide sequences is a pressing need in the field of precision and personalized medicine. In this work the detection of a unique genetic marker for hepatitis B virus with a low detection limit of 5 × 10−12m and a fast assay time is reported. In the assay presented here, fluorescently labeled liposomes are captured by gold‐decorated polystyrene beads using the target sequence as the building block. This is achieved by modifying the microbeads and the liposomes each with half of the complementary sequence. After a short incubation time with the target of interest, the fluorescent signal of the bridged bead–liposome assembly is detected via flow cytometry. Each liposome is labeled with ≈48 lipid soluble dyes resulting in an amplified fluorescent signal for every sensing event. The detection of the viral marker is even possible in an artificial serum‐spiked sample.

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Molecular beacon-functionalized gold nanoparticles as probes in dry-reagent strip biosensor for DNA analysis

Cited by 85 publications

References 17 publications

A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA

Untitled

Amplified Detection of a Unique Genomic Viral Marker Using Fluorescently Labeled Liposomes

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