Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells

Sulpizio, M.J.; Falone, Stefano; Amicarelli, Fernanda; Marchisio, Marco; Giuseppe, Fabrizio Di; Eleuterio, Enrica; Ilio, Carmine Di; Angelucci, Stefania

doi:10.1002/jcb.23310

Cited by 49 publications

(45 citation statements)

References 62 publications

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“…For instance, 7 days of continuous exposure to a 50-Hz MF at 1 mT significantly increased, the proliferation rate by 10% in the neuroblastoma cell line LAN-5 (24). Exposure to a 50-Hz ELF-MF at 1 mT also induced cytoproliferation and dedifferentiation in SH-SY5Y neuroblastoma cells, triggering overexpression of proteins related to high malignant potential, drug resistance, cytoskeleton re-arrangement and enhanced defense against oxidative stress (52). …”

Section: Discussionmentioning

confidence: 99%

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

et al. 2012

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We previously reported that intermittent exposure to a 50-Hz magnetic field (MF) at 100 μT stimulates cell proliferation in the human neuroblastoma cell line NB69. The present study aimed to investigate whether the magnetic field-induced growth promotion also occurs at a lower magnetic flux density of 10 μT. To this purpose, NB69 cells were subjected for 42 h to intermittent exposure, 3 h on/3 h off, to a 50-Hz MF at a 10 or 100 μT magnetic flux density. The field exposure took place either in the presence or in the absence of the antiproliferative agent retinoic acid. At the end of the treatment and/or incubation period, the cell growth was estimated by hemocytometric counting and spectrophotometric analysis of total protein and DNA contents. Potential changes in DNA synthesis were also assessed through proliferating cell nuclear antigen (PCNA) immunolabeling. The results confirmed previously reported data that a 42-h exposure to a 50-Hz sine wave MF at 100 μT promotes cell growth in the NB69 cell line, and showed that 10 μT induces a similar proliferative response. This effect, which was significantly associated and linearly correlated with PCNA expression, was abolished by the presence of retinoic acid in the culture medium.

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Section: Discussionmentioning

confidence: 99%

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

et al. 2012

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“…Slides were observed with a 50i Eclipse microscope (Nikon) and images were acquired with a Cool-SNAP cf digital CCD camera (PhotoMetrics, Huntington Beach, CA). Digital acquisition, processing and analysis of fluorescence were performed by Meta Image Series 7.5 (MetaMorph, Metafluor, MetaVue) software obtained from Molecular Devices [43]. …”

Section: Methodsmentioning

confidence: 99%

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

D’Alimonte

Lannutti

Pipino

et al. 2013

Stem Cell Rev and Rep

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Human amniotic fluid mesenchymal stem cells (huAFMSCs) are emerging as a promising therapeutic option in regenerative medicine. Here, we characterized huAFMSC phenotype and multipotentiality. When cultured in osteogenic medium, huAFMSC displayed a significant increase in: Alkaline Phosphatase (ALP) activity and mRNA expression, Alizarin Red S staining and Runx2 mRNA expression; whereas maintaining these cells in an adipogenic culture medium gave a time-dependent increase in PPARγ and FABP4 mRNA expression, glycerol-3-phosphate dehydrogenase (GPDH) activity and positivity to Oil Red Oil staining. These results confirm that huAFMSCs can differentiate toward osteogenic and adipogenic phenotypes. The canonical Wnt/ßcatenin signaling pathway appears to trigger huAFMSC osteoblastogenesis, since during early phases of osteogenic differentiation, the expression of Dishevelled-2 (Dvl-2), of the non-phosphorylated form of ß-catenin, and the phosphorylation of glycogen synthase kinase-3ß (GSK3ß) at serine 9 were upregulated. On the contrary, during adipogenic differentiation Dvl-2 expression decreased, whereas that of ß-catenin remained unchanged. This was associated with a late increase in GSK3ß phosphorylation. Consistent with this scenario, huAFMSCs exposure to Dickkopf-1, a selective inhibitor of the Wnt signaling, abolished Runx2 and ALP mRNA upregulation during huAFMSC osteogenic differentiation, whereas it enhanced FABP4 expression in adipocyte-differentiating cells. Taken together, these results unravel novel molecular determinants of huAFMSC commitment towards osteoblastogenesis, which may represent potential targets for directing the differentiation of these cells and improving their use in regenerative medicine.FigureSchematic representation of Wnt pathway involved in the osteogenic and adipogenic differentiation of huAFMSCs. Our paper demonstrates that osteogenic commitment of these cells is linked to the stimulation of Wnt signal leading to the final transcriptional activation of early osteogenic markers such as RUNX-2 and ALP, mediated by β-catenin. DKK1 is a secreted Wnt antagonist that may be used as a drug to inhibit Wnt signal. In contrast, adipogenic commitment involves early inhibition of Wnt pathway leading to ubiquitination/degradation of β-catenin. This results in the transcription of PPARγ and FABP4, considered as the main initiators of adipogenesis. APC, adenomatous polyposis coli; βcat, β-catenin; CK1, casein kinase 1; DKK1, dickkopf 1; Dvl, Dishevelled; GSK3β, glycogen synthase kinase 3β; LRP5/6, low density lipoprotein receptor-related protein 5/6

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“…reported that a 50 Hz MF at 1.0 mT did not elicit alterations in cell cycle progression or cellular viability in neuroblastoma BE(2)C cells [41]. Previous studies have investigated the effects of ELF-MF exposure on cellular proliferation in various neurogenic cell types, such as SH-SY5Y cells, but have shown inconsistent results with respect to cellular behaviors [42, 43]. Sulpizio et al .…”

Section: Discussionmentioning

confidence: 99%

“…Sulpizio et al . reported that 1.0 mT of 50 Hz MF altered the proliferative status, cell growth pattern, and cytoskeletal organization of proliferating SH-SY5Y cells, due to significant changes in the expression levels of common proteins involved in cellular defense mechanisms, organization and biogenesis [42]. A study by Benassi et al .…”

Section: Discussionmentioning

confidence: 99%

The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells

Yimaer

Wei

et al. 2017

Journal of Radiation Research

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Epidemiological studies have indicated a possible association between extremely low–frequency magnetic field (ELF-MF) exposure and the risk of nervous system diseases. However, laboratory studies have not provided consistent results for clarifying this association, despite many years of studies. In this study, we have systematically investigated the effects of 50 Hz MF exposure on DNA damage and cellular functions in both neurogenic tumor cell lines (U251, A172, SH-SY5Y) and primary cultured neurogenic cells from rats (astrocytes, microglia, cortical neurons). The results showed that exposure to a 50 Hz MF at 2.0 mT for up to 24 h did not influence γH2AX foci formation (an early marker of DNA double-strand breaks) in any of six different neurogenic cells. Exposure to a 50 Hz MF did not affect cell cycle progression, cell proliferation or cell viability in neurogenic tumor U251, A172 or SH-SY5Y cells. Furthermore, the MF exposure for 24 h did not significantly affect the secretion of cytokines (TNF-α, IL-6 or IL-1β) in astrocytes or microglia, or the phagocytic activity of microglia. In addition, MF exposure for 1 h per day did not significantly influence expression levels of microtubule-associated protein tau, microtubule-associated protein 2, postsynaptic density 95 or gephyrin in cortical neurons, indicating an absence of effects of MF exposure on the development of cortical neurons. In conclusion, our data suggest that exposure to a 50 Hz MF at 2.0 mT did not elicit DNA damage effects or abnormal cellular functions in the neurogenic cells studied.

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Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells

Cited by 49 publications

References 62 publications

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

Retinoic acid inhibits the cytoproliferative response to weak 50-Hz magnetic fields in neuroblastoma cells

Wnt Signaling Behaves as a “Master Regulator” in the Osteogenic and Adipogenic Commitment of Human Amniotic Fluid Mesenchymal Stem Cells

The effects of 50 Hz magnetic field exposure on DNA damage and cellular functions in various neurogenic cells

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