Molecular basis of the high-palmitic acid trait in sunflower seed oil

Pérez‐Vich, Begoña; Moral, Lidia del; Velasco, Leonardo; Bushman, B. Shaun; Knapp, Steven J.; Leόn, Alberto J.; Fernández‐Martínez, José M.; Berry, Simon

doi:10.1007/s11032-016-0462-2

Cited by 10 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lipidome profiling by UPLC-MS has already been used as an input in GWAS for several plants, for example, maize [ 32 ]. However, in sunflower, associated regions have only been identified for some of the most abundant FAs [ 33 – 35 ]. Other minor fatty acids constituting sunflower oil lipids, however, were not considered for association mapping.…”

Section: Introductionmentioning

confidence: 99%

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

et al. 2021

View full text Add to dashboard Cite

Background Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding. Results We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes for 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them. Identified genomic regions included the loci involved in rare fatty acids variation on chromosomes 3 and 14, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil. Conclusions This is the first large scale implementation of high-throughput lipidomic profiling to sunflower germplasm characterization. This study contributes to the genetic characterization of Russian sunflower collections, which made a substantial contribution to the development of sunflower as the oilseed crop worldwide, and provides new insights into the genetic control of oil composition that can be implemented in future studies.

show abstract

Section: Introductionmentioning

confidence: 99%

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

et al. 2021

View full text Add to dashboard Cite

show abstract

“…We also asked if DEGs were enriched within known sunflower oil QTL from previous mapping studies. We determined the genomic location of DEGs relative to 51 oil QTL from previously published mapping studies in cultivated sunflower and 8 oil QTL from a wild and cultivated sunflower cross (Badouin et al, 2017; Burke et al, 2005; Ebrahimi et al, 2008; Pérez-Vich et al, 2016; Premnath et al, 2016). There were 5,145 expressed genes located within oil QTL, 759 of which were DEGs.…”

Section: Resultsmentioning

confidence: 99%

“…We determined the locations of DEGs relative to the positions of previously mapped QTL related to fatty acid biosynthesis that were compiled in Badouin et al (2017) from several other previously published works (Ebrahimi et al, 2008; Pérez-Vich et al, 2016; Premnath et al, 2016). The HanXRQv1 base pair coordinates of QTL from those three studies were compiled in Badouin et al (2017) and used in this study.…”

Section: Methodsmentioning

confidence: 99%

Transcriptomics of developing wild sunflower seeds from the extreme ends of a latitudinal gradient differing in seed oil composition

Dittmar

et al. 2021

Preprint

View full text Add to dashboard Cite

Seed oil composition, an important agronomic trait in cultivated sunflower, varies latitudinally across the native range of its wild progenitor. This pattern is thought to be driven by selection for a higher proportion of saturated fatty acids in southern populations compared to northern populations, likely due to the different temperatures experienced during seed germination. To investigate whether these differences in fatty acid composition between northern and southern populations correspond to transcriptional variation in the expression of genes involved in fatty acid metabolism, we sequenced RNA from developing seeds of sunflowers from Texas, USA and Saskatchewan, Canada (the extreme ends of sunflower’s latitudinal range) grown in a common garden. Over 4,000 genes were found to be differentially expressed between Texas and Canada, including several genes involved in lipid metabolism. Many differentially expressed oil metabolism genes colocalized with known oil QTL. The genes producing stearoyl-ACP-desaturases (SAD) were of particular interest because of their known role in the conversion of fully saturated into unsaturated fatty acids. Two SAD genes were more highly expressed in seeds from Canadian populations, consistent with the observation of increased levels of unsaturated fatty acids in seeds from that region. We also constructed a gene co-expression network to investigate regional variation in network modules. The results of this analysis revealed regional differentiation for eight of twelve modules, but no clear relationship with oil biosynthesis. Overall, the differential expression of SAD genes offers a partial explanation for the observed differences in seed oil composition between Texas and Canada, while the expression patterns of other metabolic genes suggest complex regulation of fatty acid production and usage across latitudes.

show abstract

“…We therefore produced an Arabidopsis HPHO multi-mutant background with ~20% 16:0 and ~70% 18:1 by combining fab1-1 (Wu et al, 1994), fae1 (James et al, 1995) and fad2 (Miquel and Browse, 1992) alleles. This approach is also achievable in oilseed crops since Fernández-Martínez et al, (1997) previously developed a conventional HPHO sunflower (Helianthus annuus) variety with seeds containing ~30% 16:0 and ~55% 18:1 by combining fab1 and fad2 mutant alleles (Perez-Vich et al, 2016;Schuppert et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Production of the infant formula ingredient 1,3-olein-2-palmitin in Arabidopsis seeds

Erp

Bryant

Martin-Moreno³

et al. 2020

Preprint

View full text Add to dashboard Cite

In human milk fat, palmitic acid (16:0) is esterified to the middle (sn-2 or β) position on the glycerol backbone and oleic acid (18:1) predominantly to the outer positions, giving the triacylglycerol (TG) a distinctive stereoisomeric structure that is believed to assist nutrient absorption in the infant gut. However, the fat used in most infant formulas is derived from plants, which preferentially esterify 16:0 to the outer positions. We have previously showed that the metabolism of the model oilseed Arabidopsis thaliana can be engineered to incorporate 16:0 into the middle position of TG. However, the fatty acyl composition of Arabidopsis seed TG does not mimic human milk, which is rich in both 16:0 and 18:1 and is defined by the high abundance of the TG molecular species 1,3-olein-2-palmitin (OPO). Here we have constructed an Arabidopsis fatty acid biosynthesis 1-1 fatty acid desaturase 2 fatty acid elongase 1 mutant with around 20% 16:0 and ~70% 18:1 in its seeds and we have engineered it to esterify more than 80% of the 16:0 to the middle position of TG, using heterologous expression of the human lysophosphatidic acid acyltransferase isoform AGPAT1, combined with suppression of LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE 2 and PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL CHOLINEPHOSPHOTRANSFERASE. Our data suggest that oilseeds can be engineered to produce TG that is rich in OPO, which is an important structured fat ingredient used in infant formulas.

show abstract

Molecular basis of the high-palmitic acid trait in sunflower seed oil

Cited by 10 publications

References 52 publications

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

Transcriptomics of developing wild sunflower seeds from the extreme ends of a latitudinal gradient differing in seed oil composition

Production of the infant formula ingredient 1,3-olein-2-palmitin in Arabidopsis seeds

Contact Info

Product

Resources

About