Molecular basis of resistance to macrolides and lincosamides among staphylococci and streptococci from various animal sources collected in the resistance monitoring program BfT-GermVet

Lüthje, Petra; Schwarz, Štefan

doi:10.1016/j.ijantimicag.2006.12.016

Cited by 67 publications

(46 citation statements)

References 38 publications

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“…Previously established MIC breakpoints were used (13,14,18,27). The presence of the following resistance genes was determined by PCR: tetracycline resistance genes (30), and tet(W) (31); macrolide-lincosamide-streptogramin B (MLS B ) resistance genes erm(A), erm(B), (32), erm(C) (33), mef(A) and/or mef(E) (32), mph(C) (34), msr(A) and/or msr(B) (35), and msr(D) (36); phenicol resistance genes fexA and cfr (37) (38). Finally, the chromosomal mutations within the quinolone resistance-determining region (QRDR) of the gyrA and parC genes were determined in a subset of fluoroquinolone-resistant S. suis isolates (n ϭ 55), as described previously (19).…”

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confidence: 99%

Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

et al. 2015

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confidence: 99%

Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

et al. 2015

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“…Resistance to lincosamides only (L phenotype) is exclusively based on the inactivation of lincosamides by nucleotidylation via Onucleotidyltransferases (6). Since the first report of a lincosamide nucleotidyltransferase gene, lnu(A) (formerly linA) (7), a number of lnu genes have been described in different bacteria from animal, human, and environmental sources, including lnu(B) (8), lnu(C) (9), lnu(D) (10), and lnu(F) (11). Most of these lnu genes confer resistance to lincomycin and pirlimycin, but their MICs for clindamycin are often below the breakpoints for resistance (2,9,10).…”

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Identification of the Novel Lincosamide Resistance Gene lnu (E) Truncated by ISEnfa5-cfr-IS Enfa5 Insertion in Streptococcus suis: De Novo Synthesis and Confirmation of Functional Activity in Staphylococcus aureus

Zhao

Wendlandt

et al. 2014

Antimicrob Agents Chemother

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bThe novel lincosamide resistance gene lnu(E), truncated by insertion of an ISEnfa5-cfr-ISEnfa5 segment, was identified in Streptococcus suis. The gene lnu(E) encodes a 173-amino-acid protein with <69.4% identity to other lincosamide nucleotidyltransferases. The lnu(E) gene and its promoter region were de novo synthesized, and Staphylococcus aureus RN4220 carrying a shuttle vector with the cloned lnu(E) gene showed a 16-fold increase in the lincomycin MIC. Mass spectrometry experiments demonstrated that Lnu(E) catalyzed the nucleotidylation of lincomycin.

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“…Cli belongs to the family of Lincosamides, and according to Moellering (1991) resistance to such antimicrobial is common in enterococci, which may be considered a case of intrinsic resistance, with interspecies associations. For Lüthje and Schwarz (2007), bacterial resistance to lincosamide is related to efflux mechanisms and mutations. The susceptibility of enterococcal isolates to clindamycin was reported by Schmitz et al (1999) -only 4.4 of all E. faecalis strains from 20 European university hospitals were clindamycin-susceptible.…”

Section: Discussionmentioning

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Multiple antibiotic-resistance of Enterococcus isolated from coastal water near an outfall in Brazil

Edirsana¹,

Renata²,

Alberto³

et al. 2014

Afr. J. Microbiol. Res.

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Marine outfalls contribute to the environmental protection of coastal zones. However, these structures may serve as vehicles for microbiological contamination. This study aimed to investigate the occurrence of antimicrobial-resistant bacteria in water samples collected from 67 stations located in nearby areas of the ocean outfall in Fortaleza, Brazil. 81 Enterococcus strains were isolated, identified and distributed in the following groups of species: Enterococcus faecalis (n = 37; 45.7%), Enterococcus faecium (n = 30; 37%), Enterococcus mundtii (n = 9; 11.1%), Enterococcus raffinosus (n = 2; 2.5%), Enterococcus dispar (n = 2; 2.5%) and Enterococcus durans (n = 1; 1.2%). Antimicrobial resistance was observed in 47 (58%) of the strains, and the most predominant profile was the concurrent resistance to ampicillin, clindamycin, penicillin and vancomycin. In 31 strains were detected phenotypically, plasmid resistance factors. The data reported in this study should serve as an alert to public health authorities, since they suggest that the area near the submarine outfall in Fortaleza may contribute to antimicrobialresistant enterococci spread.

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Molecular basis of resistance to macrolides and lincosamides among staphylococci and streptococci from various animal sources collected in the resistance monitoring program BfT-GermVet

Cited by 67 publications

References 38 publications

Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis

Identification of the Novel Lincosamide Resistance Gene lnu (E) Truncated by ISEnfa5-cfr-IS Enfa5 Insertion in Streptococcus suis: De Novo Synthesis and Confirmation of Functional Activity in Staphylococcus aureus

Multiple antibiotic-resistance of Enterococcus isolated from coastal water near an outfall in Brazil

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