Molecular Basis of Replication of Duck H5N1 Influenza Viruses in a Mammalian Mouse Model

Li, Zejun; Chen, Hualan; Jiao, Peirong; Deng, Guohua; Tian, Guobin; Li, Yanbing; Hoffmann, Erich; Webster, Robert G.; Matsuoka, Yumiko; Yu, Kangzhen

doi:10.1128/jvi.79.18.12058-12064.2005

Cited by 499 publications

(500 citation statements)

References 30 publications

(22 reference statements)

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“…Several amino acid changes in PB2 are important for the replication or virulence of influenza viruses in mammals (22)(23)(24)(25)(26)(27)(28)(29)(30). A detailed comparison of the amino acid differences among the EAH1N1 SIVs indicated that all of these viruses have mammalian-adapting mutations in their PB2 genes (SI Appendix, Table S3).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses

Yang

Chen

Qiao

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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104

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Pigs are important intermediate hosts for generating novel influenza viruses. The Eurasian avian-like H1N1 (EAH1N1) swine influenza viruses (SIVs) have circulated in pigs since 1979, and human cases associated with EAH1N1 SIVs have been reported in several countries. However, the biologic properties of EAH1N1 SIVs are largely unknown. Here, we performed extensive influenza surveillance in pigs in China and isolated 228 influenza viruses from 36,417 pigs. We found that 139 of the 228 strains from pigs in 10 provinces in China belong to the EAH1N1 lineage. These viruses formed five genotypes, with two distinct antigenic groups, represented by A/swine/Guangxi/18/2011 and A/swine/Guangdong/104/2013, both of which are antigenically and genetically distinct from the current human H1N1 viruses. Importantly, the EAH1N1 SIVs preferentially bound to human-type receptors, and 9 of the 10 tested viruses transmitted in ferrets by respiratory droplet. We found that 3.6% of children (≤10 y old), 0% of adults, and 13.4% of elderly adults (≥60 y old) had neutralization antibodies (titers ≥40 in children and ≥80 in adults) against the EAH1N1 A/swine/Guangxi/18/2011 virus, but none of them had such neutralization antibodies against the EAH1N1 A/swine/Guangdong/104/2013 virus. Our study shows the potential of EAH1N1 SIVs to transmit efficiently in humans and suggests that immediate action is needed to prevent the efficient transmission of EAH1N1 SIVs to humans.

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Section: Discussionmentioning

confidence: 99%

Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses

Yang

Chen

Qiao

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

104

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“…As described (30), all eight Vic75 genes were amplified and cloned into a viral RNA (vRNA) expression plasmid, and Vic75 PB2, PB1, PA, and NP genes were cloned into an mRNA expression plasmid. All eight genes of HK486 were amplified from vRNA by RT-PCR with a One-Step RT-PCR kit (Qiagen, Valencia, CA) and cloned into a bidirectional plasmid (pBD) that possesses vRNA expression elements from pPolISapIRib (31,32) and mRNA expression elements from pCI (Promega, Madison, WI). Reassortant viruses were rescued as described (33) by using the 12-plasmid Vic75 system, the eight-plasmid HK486 system, or a combination of the two.…”

Section: Methodsmentioning

confidence: 99%

Lack of transmission of H5N1 avian–human reassortant influenza viruses in a ferret model

Maines

Chen

Matsuoka

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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316

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Avian influenza A H5N1 viruses continue to spread globally among birds, resulting in occasional transmission of virus from infected poultry to humans. Probable human-to-human transmission has been documented rarely, but H5N1 viruses have not yet acquired the ability to transmit efficiently among humans, an essential property of a pandemic virus. The pandemics of 1957 and 1968 were caused by avian-human reassortant influenza viruses that had acquired human virus-like receptor binding properties. However, the relative contribution of human internal protein genes or other molecular changes to the efficient transmission of influenza viruses among humans remains poorly understood. Here, we report on a comparative ferret model that parallels the efficient transmission of H3N2 human viruses and the poor transmission of H5N1 avian viruses in humans. In this model, an H3N2 reassortant virus with avian virus internal protein genes exhibited efficient replication but inefficient transmission, whereas H5N1 reassortant viruses with four or six human virus internal protein genes exhibited reduced replication and no transmission. These findings indicate that the human virus H3N2 surface protein genes alone did not confer efficient transmissibility and that acquisition of human virus internal protein genes alone was insufficient for this 1997 H5N1 virus to develop pandemic capabilities, even after serial passages in a mammalian host. These results highlight the complexity of the genetic basis of influenza virus transmissibility and suggest that H5N1 viruses may require further adaptation to acquire this essential pandemic trait. transmissibility ͉ pandemic virus properties ͉ pandemic influenza ͉ animal model ͉ receptor specificity

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“…The 627K and 701N residues in the PB2 protein contribute to the replication and transmission of avian influenza viruses in mammalian hosts [11][12][13][14]. The three avian and environmental H7N9 viruses had the avian-like 627E residue.…”

Section: Molecular Signatures Of the Novel H7n9 Virusesmentioning

confidence: 99%

Isolation and characterization of H7N9 viruses from live poultry markets — Implication of the source of current H7N9 infection in humans

et al. 2013

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Molecular Basis of Replication of Duck H5N1 Influenza Viruses in a Mammalian Mouse Model

Cited by 499 publications

References 30 publications

Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses

Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses

Lack of transmission of H5N1 avian–human reassortant influenza viruses in a ferret model

Isolation and characterization of H7N9 viruses from live poultry markets — Implication of the source of current H7N9 infection in humans

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