Molecular basis of potassium channels in pancreatic duct epithelial cells

Hayashi, Mikio; Novak, Ivana

doi:10.4161/chan.26100

Cited by 40 publications

(36 citation statements)

References 146 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, luminal K + channels could contribute to secreted K + , as pancreatic juice contains 4–8 mM K + (Sewell and Young, 1975; Caflisch et al, 1979; Seow et al, 1991). The molecular identity of some K + channels in pancreatic ducts is known, however, the exact localization and function remains to be verified [see (Hayashi and Novak, 2013)]. The K Ca 1.1 channels (maxi-K, BK, coded by KCNMA1 ) are present in pancreatic ducts (Hede et al, 2005; Venglovecz et al, 2011).…”

Section: Novel Ion Channels and Pumps Contributing To Acid-base Transmentioning

confidence: 99%

Acid-base transport in pancreas—new challenges

2013

Self Cite

View full text Add to dashboard Cite

Along the gastrointestinal tract a number of epithelia contribute with acid or basic secretions in order to aid digestive processes. The stomach and pancreas are the most extreme examples of acid (H+) and base (HCO−3) transporters, respectively. Nevertheless, they share the same challenges of transporting acid and bases across epithelia and effectively regulating their intracellular pH. In this review, we will make use of comparative physiology to enlighten the cellular mechanisms of pancreatic HCO−3 and fluid secretion, which is still challenging physiologists. Some of the novel transporters to consider in pancreas are the proton pumps (H+-K+-ATPases), as well as the calcium-activated K+ and Cl− channels, such as KCa3.1 and TMEM16A/ANO1. Local regulators, such as purinergic signaling, fine-tune, and coordinate pancreatic secretion. Lastly, we speculate whether dys-regulation of acid-base transport contributes to pancreatic diseases including cystic fibrosis, pancreatitis, and cancer.

show abstract

Section: Novel Ion Channels and Pumps Contributing To Acid-base Transmentioning

confidence: 99%

Acid-base transport in pancreas—new challenges

2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…More than 30 years ago, the existence of native sodium‐activated potassium currents was demonstrated through electrophysiological experiments in guinea pig cardiomyocytes (Kameyama et al, ). Since then, the presence of native sodium‐activated potassium channels has been reported in diverse myocytes (Kim et al, ; Re et al, ), pancreatic duct epithelial cells (Hayashi and Novak, ), thick ascending limb of Henle's loop in the kidney (Paulais et al, ), various mammalian neurons (Egan et al, ; Kaczmarek, ), and Xenopus oocytes (Egan et al, ). At present, two distinct ion channels giving rise to an outward rectifying potassium current activated upon rises in internal sodium ions have been described (Bhattacharjee and Kaczmarek, ; Salkoff et al, ; Yuan et al, ).…”

mentioning

confidence: 99%

Differential distribution of the sodium‐activated potassium channels slick and slack in mouse brain

Rizzi

Knaus

Schwarzer

2015

J of Comparative Neurology

View full text Add to dashboard Cite

The sodium‐activated potassium channels Slick (Slo2.1, KCNT2) and Slack (Slo2.2, KCNT1) are high‐conductance potassium channels of the Slo family. In neurons, Slick and Slack channels are involved in the generation of slow afterhyperpolarization, in the regulation of firing patterns, and in setting and stabilizing the resting membrane potential. The distribution and subcellular localization of Slick and Slack channels in the mouse brain have not yet been established in detail. The present study addresses this issue through in situ hybridization and immunohistochemistry. Both channels were widely distributed and exhibited distinct distribution patterns. However, in some brain regions, their expression overlapped. Intense Slick channel immunoreactivity was observed in processes, varicosities, and neuronal cell bodies of the olfactory bulb, granular zones of cortical regions, hippocampus, amygdala, lateral septal nuclei, certain hypothalamic and midbrain nuclei, and several regions of the brainstem. The Slack channel showed primarily a diffuse immunostaining pattern, and labeling of cell somata and processes was observed only occasionally. The highest Slack channel expression was detected in the olfactory bulb, lateral septal nuclei, basal ganglia, and distinct areas of the midbrain, brainstem, and cerebellar cortex. In addition, comparing our data obtained from mouse brain with a previously published study on rat brain revealed some differences in the expression and distribution of Slick and Slack channels in these species. J. Comp. Neurol. 524:2093–2116, 2016. © 2015 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

show abstract

“…An electrophysiological study indicated that KCNK5 was expressed in human pancreatic ductal adenocarcinoma cell line, and the pH-sensitive K2P subunits coded by KCNK5 were shown to be expressed in pancreatic. 20 Transcriptome-wide association study analysis identified several novel candidate genes for PC, such as RP11-65J3.1, PPFIBP2, GEMIN4, NIPA2,RNASEH2B, FARS2, MTHFD1L, F2R, TXNDC15, NDUFA3, CRISPLD2, IQSEC3, and LRP5L. As is known to all, few efforts have been paid to investigate the potential roles of them in the formation of PC.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome‐wide association study identifies multiple genes and pathways associated with pancreatic cancer

et al. 2018

View full text Add to dashboard Cite

AimTo identify novel candidate genes for pancreatic cancer.MethodsWe performed a transcriptome‐wide association study (TWAS) analysis of pancreatic cancer (PC). GWAS summary data were driven from the published studies of PC, totally involving 558 542 SNPs in 1896 individuals with pancreatic cancer and 1939 healthy controls. FUSION software was applied to the PC GWAS summary data for tissue‐related TWAS analysis, including whole blood, peripheral blood, adipose, and pancreas. The functional relevance of identified genes with PC was further validated by Oncomine, STRING, and CluePedia tool.ResultsTranscriptome‐wide association study analysis identified 19 genes significantly associated with PC, such as LRP5L (P value = 5.21 × 10‐5), SOX4 (P value = 3.2 × 10‐4), and EGLN3 (P value = 6.2 × 10‐3). KEGG pathway enrichment analysis detected several PC‐associated pathways, such as One carbon pool by folate (P value = 1.60 × 10‐16), Cell cycle (P value = 1.27 × 10‐7), TGF‐beta signaling pathway (P value = 4.64 × 10‐6). Further comparing the 19 genes with previously identified overexpressed genes in PC patients found one overlapped gene SOX4.ConclusionWe identified some novel candidate genes and pathways associated with PC. Our results provide novel clues for the genetic mechanism studies of pancreatic cancer.

show abstract

Molecular basis of potassium channels in pancreatic duct epithelial cells

Cited by 40 publications

References 146 publications

Acid-base transport in pancreas—new challenges

Acid-base transport in pancreas—new challenges

Differential distribution of the sodium‐activated potassium channels slick and slack in mouse brain

Transcriptome‐wide association study identifies multiple genes and pathways associated with pancreatic cancer

Contact Info

Product

Resources

About