Molecular basis of Iron Biofortification in crop plants; A step towards sustainability

Rehman, Attiq Ur; Masood, Sara; Khan, Naqib Ullah; Ejaz, Muhammad; Hussain, Zawar; Ali, Ihtisham

doi:10.1111/pbr.12886

Cited by 27 publications

(16 citation statements)

References 84 publications

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“…Our previous study demonstrated that the chlorosis phenotype was caused by iron deficiency in citrus plants [ 20 , 22 ]. To explore the differences in iron acquisition between the two citrus rootstock species (ZQ and TO), we first investigated the activity of ferric chelate reductase (FCR), responsible for iron reduction, upon iron-deficiency treatment for 1 week, since iron reduction (step 2) has been reported as the rate-limiting step for iron uptake [ 23 ]. The results show that FCR activity is slightly lower in ZQ than TO, though no significant difference was found ( Fig.…”

Section: Resultsmentioning

confidence: 99%

MYB308-mediated transcriptional activation of plasma membrane H+-ATPase 6 promotes iron uptake in citrus

Zhengyan

Liuying

et al. 2022

Horticulture Research

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Iron-deficiency chlorosis is a common nutritional disorder in crops grown on alkaline or calcareous soils. Although the acclimation mechanism to iron deficiency has been investigated, the genetic regulation of iron acquisition is still unclear. Here, by comparing iron uptake process between an iron-poor-soil-tolerant citrus species Zhique (ZQ) and an iron-poor-soil sensitive citrus species trifoliate orange (TO), we discovered the enhanced root H+ efflux is crucial for the tolerance to iron deficiency in ZQ. The H+ efflux is mainly regulated by a plasma membrane localized H+-ATPase HA6, the expression of which is up-regulated in plants grown in soil with low iron content and significantly higher in the roots of ZQ than TO. Overexpression of the HA6 gene in Arabidopsis thaliana aha2 mutant defective in iron uptake recovered the wild type phenotype. In parallel, overexpression of the HA6 gene in TO significantly increased iron content of plants. Moreover, an iron deficiency-induced transcription factor MYB308 was revealed to bind the promoter and activate the expression of HA6 in ZQ by yeast one-hybrid, electrophoretic mobility shift and dual-luciferase assays. Overexpression of MYB308 in ZQ roots significantly increased the expression level of HA6 gene. However, MYB308 can not bind or activate the HA6 promoter in TO due to the sequence variation of corresponding MYB308 binding motif. Together, we propose that the MYB308 could activate HA6 to promote root H+ efflux and iron uptake, and the distinctive MYB308-HA6 transcriptional module may be, at least in part, responsible for the iron deficiency tolerance in citrus.

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Section: Resultsmentioning

confidence: 99%

MYB308-mediated transcriptional activation of plasma membrane H+-ATPase 6 promotes iron uptake in citrus

Zhengyan

Liuying

et al. 2022

Horticulture Research

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“…This need is linked to the lack of essential elements in human diets [ 23 , 44 , 45 ]. As a consequence, biofortification is recognized as a useful tool to enhance the concentration of trace elements, such as I [ 19 , 28 , 30 ], zinc [ 23 , 46 , 47 ], selenium [ 48 , 49 , 50 , 51 ], manganese [ 52 , 53 , 54 ], molybdenum [ 14 , 55 , 56 , 57 , 58 ], iron [ 59 , 60 , 61 ], and bioactive compounds in fruits and vegetables. Inadequate I intakes can create I deficiency disorders (IDD) in humans, with considerable consequences on life quality [ 62 , 63 , 64 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Iodine Biofortification and Seaweed Extract-Based Biostimulant Supply Interactively Drive the Yield, Quality, and Functional Traits in Strawberry Fruits

Consentino

Vultaggio

Iacuzzi

et al. 2023

Plants

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The horticultural sector is seeking innovative and sustainable agronomic practices which could lead to enhanced yield and product quality. Currently, plant biofortification is recognized as a valuable technique to improve microelement concentrations in plant tissues. Among trace elements, iodine (I) is an essential microelement for human nutrition. Concomitantly, the application of biostimulants may improve overall plant production and quality traits. With the above background in mind, an experiment was designed with the aim of assessing the interactive impact of a seaweed extract-based biostimulant (SwE) (0 mL L−1 (served as control) or 3 mL L−1 (optimal dosage)) and 0, 100, 300, or 600 mg L−1 I on the growth parameters, yield, fruit quality, minerals, and functional characteristics of the tunnel-grown “Savana” strawberry. SwE foliar application improved the plant growth-related traits, total and marketable yield, fruit color parameters, soluble solids content, nitrogen (N), potassium (K), and magnesium (Mg) fruit concentrations. Furthermore, an enhancement in the fruit dry matter content, ascorbic acid, and I concentration in fruits was detected when the SwE supply interacted with a mild I dose (100 or 300 mg L−1). The research underlined that combining SwE application and I biofortification increased the strawberry yield and quality and enhanced the plant nutritional status variation, thereby, determining a boosted strawberry I tolerance.

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“…Indeed, micronutrient malnutrition is still a significant public health problem that affects about one-third of the world’s population ( Thompson and Amoroso, 2014 ). Therefore, several staple crops have been biofortified to accumulate various micronutrients, including iron, zinc and provitamin A ( McGuire, 2015 ; Cakmak and Kutman, 2017 ; Wakeel et al., 2018 ; Zheng et al., 2020 ; Rehman et al., 2021 ). Vitamin A deficiency (VAD) is the major reason for childhood blindness and mortality, particularly impacting preschool children ( West and Darnton-Hill, 2008 ; Greiner, 2013 ).…”

Section: Carotenoid Biofortification In Plantsmentioning

confidence: 99%

Carotenoid metabolism: New insights and synthetic approaches

et al. 2023

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Carotenoids are well-known isoprenoid pigments naturally produced by plants, algae, photosynthetic bacteria as well as by several heterotrophic microorganisms. In plants, they are synthesized in plastids where they play essential roles in light-harvesting and in protecting the photosynthetic apparatus from reactive oxygen species (ROS). Carotenoids are also precursors of bioactive metabolites called apocarotenoids, including vitamin A and the phytohormones abscisic acid (ABA) and strigolactones (SLs). Genetic engineering of carotenogenesis made possible the enhancement of the nutritional value of many crops. New metabolic engineering approaches have recently been developed to modulate carotenoid content, including the employment of CRISPR technologies for single-base editing and the integration of exogenous genes into specific “safe harbors” in the genome. In addition, recent studies revealed the option of synthetic conversion of leaf chloroplasts into chromoplasts, thus increasing carotenoid storage capacity and boosting the nutritional value of green plant tissues. Moreover, transient gene expression through viral vectors allowed the accumulation of carotenoids outside the plastid. Furthermore, the utilization of engineered microorganisms allowed efficient mass production of carotenoids, making it convenient for industrial practices. Interestingly, manipulation of carotenoid biosynthesis can also influence plant architecture, and positively impact growth and yield, making it an important target for crop improvements beyond biofortification. Here, we briefly describe carotenoid biosynthesis and highlight the latest advances and discoveries related to synthetic carotenoid metabolism in plants and microorganisms.

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Molecular basis of Iron Biofortification in crop plants; A step towards sustainability

Cited by 27 publications

References 84 publications

MYB308-mediated transcriptional activation of plasma membrane H+-ATPase 6 promotes iron uptake in citrus

MYB308-mediated transcriptional activation of plasma membrane H+-ATPase 6 promotes iron uptake in citrus

Iodine Biofortification and Seaweed Extract-Based Biostimulant Supply Interactively Drive the Yield, Quality, and Functional Traits in Strawberry Fruits

Carotenoid metabolism: New insights and synthetic approaches

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