Molecular basis of floral petaloidy: insights from androecia of Canna indica

Fu, Qing; Liu, Huanfang; Almeida, Ana Maria Rocha de; Kuang, Yuanwen; Zou, Pu; Liao, Jingping

doi:10.1093/aobpla/plu015

Cited by 11 publications

(14 citation statements)

References 47 publications

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“…RNA-Seq and in situ hybridization results showed that C-function unigene CiAG-1 was highly expressed in PS, while showed much lower or no expression in P. The combined expression of CiGLO-1 and CiAG-1 in the whorl 3 suggests that the petaloid staminodes are of androecial identity, which is in accordance with the traditional view, and with the case in A. hainanensis (Zingiberaceae), another Zingiberales species (Song et al, 2010 ). However, this evidence was not sufficient to explain the different fates of the androecial members since co-expression of B- and C-class genes specifies stamen identity (Fu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…In C. indica , transcripts of CiGLO-1 were not detected in the carpel primordia at early stages, while were detectable in the style primordium later. It seems that B-class is not involved in initiation and identity determination of carpels, while plays a role in the partially petaloid morphogenesis of the style (Glinos and Cocucci, 2011 ; Fu et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…As implied by the name, petaloid staminodes are thought to have evolved from stamens. Recently, many efforts have been made to reveal the molecular basis for androecial petaloidy of Zingiberales species including C. indica , yet the exact mechanism remains unclear (Bartlett and Specht, 2010 ; Song et al, 2010 ; Almeida et al, 2013 ; Yockteng et al, 2013 ; Fu et al, 2014 ; Almeida et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…According to the ABC model (whether classic or updated), it is hypothesized that this kind of homeotic conversion is resulted from the absence of C-class genes in these petaloid staminodes (Wake et al, 2011 ). However, evidences in Alpinia hainanensis (Zingiberaceae) and C. indica have shown that C-class genes were still expressed in the petaloid androecial members (Song et al, 2010 ; Almeida et al, 2013 ; Fu et al, 2014 ). Furthermore, expressions of B-class GLOBOSA ( GLO , homologous to PI )-like genes were not confined within corolla and androecium (Bartlett and Specht, 2010 ), making it more complicated to explain the organ identity specification in C. indica as well as in Zingiberales.…”

Section: Introductionmentioning

confidence: 99%

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Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Tian

Liu

et al. 2016

Front. Plant Sci.

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Canna indica (Zingiberales) is one of the most important ornamental species characterized with beautiful petaloid staminodes, which are considered to evolve from stamens. However, the genetic basis for the development of petaloid staminodes remains unclear largely because the genomic sequences are not available. By using RNA-Seq, we sequenced the transcripts in the flower of C. indica, and quantified the temporal gene expressions in flower primordium and differentiated flower, as well as the spatial gene expressions in petal and petaloid staminode. In total, 118,869 unigenes were assembled, among which 67,299 unigenes were annotated. Quantification analysis identified the differentially expressed genes in the temporal and spatial two comparisons, based on which, Gene Ontology enrichment analysis highlighted the representative terms in each sample, such as specification of organ number in flower primordium, growth in differentiated flower, secondary cell wall biogenesis in petal and cell division in petaloid staminode. Among the 51 analyzed MADS-box unigenes, 37 were up-regulated in differentiated flower compared with those in flower primordium. A-class unigenes were expressed higher in petal than in petaloid staminode, and C-class unigenes were expressed oppositely, whereas B-class unigenes demonstrated close expression levels in these two organs, indicating that petaloid staminode retains stamen identity to some degree. In situ hybridization provided more detailed expression patterns of these unigenes, and revealed the extended expression of B-class to the carpel at later stages when the style turned flat. These results constitute a preliminary basis for the study of flower development in C. indica and can be applied in further study of the evolution of Zingiberales.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Tian

Liu

et al. 2016

Front. Plant Sci.

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“…Petaloidy of androecial organs is a representative character in the flowers of C. indica as well as many other Zingiberales species, and it is also an important clue for analysing the evolution of this order ( Kress et al 2001 ; Wake et al 2011 ; Specht et al 2012 ; Fu et al 2014 ). Compared with the two-thecae stamen in the Zingiberaceae, half of the stamen is replaced by a petaloid appendage in the Cannaceae ( Kirchoff 1991 ).…”

Section: Discussionmentioning

confidence: 99%

Irregular adaxial–abaxial polarity rearrangement contributes to the monosymmetric-to-asymmetric transformation of Canna indica stamen

Tian

et al. 2020

AoB PLANTS

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In flowering plants, lateral organs including stamens develop according to the precise regulation of adaxial-abaxial polarity. However, the polarity establishment process is poorly understood in asymmetric stamens. Canna indica (Zingiberales: Cannaceae) is a common ornamental plant with an asymmetric stamen comprising a one-theca anther and a petaloid appendage. In this study, we depicted the monosymmetric-to-asymmetric morphogenesis of C. indica stamen, and the morphogenesis of the monosymmetric stamen of a sister species was used as a contrast. We chose a HD-ZIP III gene family member and a YABBY family member as the adaxial and abaxial polarity marker genes respectively and tested their expression using mRNA in situ hybridization. The expression patterns of the two genes changed dynamically and asymmetrically during the stamen development process. Compared with their homologs in Arabidopsis thaliana, these two genes exhibited some specific expression patterns. We hypothesize that the distinctive adaxial-abaxial polarity participates in the irregular morphogenesis of C. indica stamen, which mediates the putative stamen-to-petaloid staminode conversion in this species.

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RNA-Seq analysis reveals the distinctive adaxial–abaxial polarity in the asymmetric one-theca stamen of Canna indica

et al. 2017

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Proper establishment of adaxial-abaxial polarity is essential for the development of lateral organs, while former researches were mostly focused on the polarity regulation in leaves, and little is known in stamens, especially in the asymmetric ones. Canna indica (Zingiberales: Cannaceae) is a widely cultivated ornamental plant and the representative species to study the evolutionary development of Zingiberales. The androecium of Canna indica comprises 3-4 petaloid staminodes and a fertile stamen (FS), which consists of a one-theca anther and a petaloid appendage. The partially petaloid stamen is considered as an intermediate state organ from a two-thecae stamen to a completely petaloid staminode. Using RNA-Seq, we quantified the expressions of the transcripts in anther and petaloid appendage, and detected 64,430 and 57,041 unigenes in these two organs, respectively. 4574 unigenes were down-regulated, and 3525 were up-regulated in petaloid appendage compared with those in anther. GO enrichment analysis indicated that the function of cytokinin is more related to cell differentiation in anther, while auxin is more to cell division in petaloid appendage. B- and C-class floral homeotic genes were expressed in these two androecium parts. Most of the class III HD-ZIP family members, which specify adaxial identity, were expressed lower in petaloid appendage than in anther; while KANADIs and YABBYs, which promote abaxial identity, exhibited opposite expression patterns. In situ hybridization showed that the adaxial marker gene was mainly expressed in the region between the two protrusions of the anther, while the abaxial marker was mainly expressed in petaloid appendage. We hypothesize that the adaxial-abaxial polarity participates in the distinctive anther-petaloid appendage patterning within the asymmetric FS of Canna indica.

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Molecular basis of floral petaloidy: insights from androecia of Canna indica

Cited by 11 publications

References 47 publications

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Temporal-Spatial Transcriptome Analyses Provide Insights into the Development of Petaloid Androecium in Canna indica

Irregular adaxial–abaxial polarity rearrangement contributes to the monosymmetric-to-asymmetric transformation of Canna indica stamen

RNA-Seq analysis reveals the distinctive adaxial–abaxial polarity in the asymmetric one-theca stamen of Canna indica

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