Molecular Basis of Familial and Acquired Phagocytosis Deficiency Involving the Tetrapeptide, Thr-Lys-Pro-Arg, Tuftsin

Najjar, Victor A.

doi:10.1159/000162886

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…The physiological significance of t u f t s i n became apparent from the observation that deficiency in the metabolism of this peptide coincides with a high incidence of infections (17).…”

Section: Introductionmentioning

confidence: 99%

In vivo immunostimulation by tuftsin

Florentin

Bruley-Rosset

Kiger

et al. 1978

Cancer Immunol Immunother

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“…The physiological significance of t u f t s i n became apparent from the observation that deficiency in the metabolism of this peptide coincides with a high incidence of infections (17).…”

Section: Introductionmentioning

confidence: 99%

In vivo immunostimulation by tuftsin

Florentin

Bruley-Rosset

Kiger

et al. 1978

Cancer Immunol Immunother

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“…In one such patient, the mutant peptide was identified as Thr-Glu-ProArg and represents a transition mutation (A--G) where codon AAA or AAG for lysine is converted to GAA or GAG, which codes for glutamic acid (18,29). Such patients suffer much aggravated multiple infections (30)(31)(32)(33)(34). In view of all these findings, tuftsin should rank as a true biological entity with a prescribed physiological role, which, when set aside, leads to physiologic impairment.…”

mentioning

confidence: 99%

Isolation and subunit composition of tuftsin receptor.

Bump¹,

Lee²,

M³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Tuftsin (Thr-Lys-Pro-Ari) receptor was purified to apparent homogeneity by affinity chromatography, using a pentapeptide analog (Thr-Lys-Pro-Pro-Arg) that binds the receptor more than 4 times as avidly as tuftsin. The analog was covalently linked to a solid support (Affi-Gel 10). Rabbit peritoneal granulocyte membrane solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-l-propanesulfonate was applied to the affinity column, the column was washed with 0.1 M ammonium carbonate (pH 7.9) and 0.1 M ammonium acetate (pH 5), and bound material was eluted with 20 nM tuftsin or pentapeptide. The eluate was concentrated and subjected to gel filtration; this yielded one major peak of [3lH]tuftsin binding activity corresponding to =500 kDa and a minor peak at -250 kDa. Rechromatography of either peak resulted in the appearance of the same maor and minor peaks. NaDodSO4/PAGE of the affinity-purified material under nonreducing conditions showed only two silver-staining bands.Electroblotting followed by [3H]tuftsin overlay and fluorography showed two adjacent radioactive bands corresponding in mobility to the silver-stained bands. Under reducing conditions, NaDodSO4/PAGE yielded molecular mass values 62 kDa and 52 kDa for the two tuftsin receptor subunits. Electron microscopy revealed a homogeneous population of spherical molecules with diameters of 104 A.

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“…Tuftsin accentuates a number of activities associated with the macrophage and neutrophil, including motility, phagocytosis, immunogenic functions, augmentation of antibody production, bactericidal activity, and antitumor effects in animal systems (112). It is generated in the serum by two enzymatic cleavages from the parent molecule, a cytophilic immunoglobulin, and repre sents amino-acid residues 289 through 292 of the constant region of the IgG heavy chain.…”

Section: Tuftsinmentioning

confidence: 99%