Molecular basis of (E)-β-farnesene-mediated aphid location in the predator Eupeodes corollae

Wang, Bing; Dong, Wanying; Li, Huimin; D’Onofrio, Chiara; Bai, Penghua; Chen, Ruipeng; Yang, Lulu; Wu, Jinlong; Wang, Xiaoqing; Wang, Bo; Ai, Dong; Knoll, Wolfgang; Pelosi, Paolo; Wang, Guirong

doi:10.1016/j.cub.2021.12.054

Cited by 44 publications

(43 citation statements)

References 61 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 72 ]. Although the α- and β-forms of farnesene are well-known pheromones [ 73 , 74 ], we did not find any reports on the biological activity of these compounds in human cells.…”

Section: Resultscontrasting

confidence: 66%

Neutrophil Immunomodulatory Activity of Farnesene, a Component of Artemisia dracunculus Essential Oils

Schepetkin

Özek

et al. 2022

Pharmaceuticals

View full text Add to dashboard Cite

Despite their reported therapeutic properties, not much is known about the immunomodulatory activity of essential oils present in Artemisia species. We isolated essential oils from the flowers and leaves of five Artemisia species: A. tridentata, A. ludoviciana, A. dracunculus, A. frigida, and A. cana. The chemical composition of the Artemisia essential oil samples had similarities and differences as compared to those previously reported in the literature. The main components of essential oils obtained from A. tridentata, A. ludoviciana, A. frigida, and A. cana were camphor (23.0–51.3%), 1,8-cineole (5.7–30.0%), camphene (1.6–7.7%), borneol (2.3–14.6%), artemisiole (1.2–7.5%), terpinen-4-ol (2.0–6.9%), α-pinene (0.8–3.9%), and santolinatriene (0.7–3.5%). Essential oils from A. dracunculus were enriched in methyl chavicol (38.8–42.9%), methyl eugenol (26.1–26.4%), terpinolene (5.5–8.8%), (E/Z)-β-ocimene (7.3–16.0%), β-phellandrene (1.3–2.2%), p-cymen-8-ol (0.9–2.3%), and xanthoxylin (1.2–2.2%). A comparison across species also demonstrated that some compounds were present in only one Artemisia species. Although Artemisia essential oils were weak activators of human neutrophils, they were relatively more potent in inhibiting subsequent neutrophil Ca2+ mobilization with N-formyl peptide receptor 1 (FPR1) agonist fMLF- and FPR2 agonist WKYMVM, with the most potent being essential oils from A. dracunculus. Further analysis of unique compounds found in A. dracunculus showed that farnesene, a compound with a similar hydrocarbon structure as lipoxin A4, inhibited Ca2+ influx induced in human neutrophils by fMLF (IC50 = 1.2 μM), WKYMVM (IC50 = 1.4 μM), or interleukin 8 (IC50 = 2.6 μM). Pretreatment with A. dracunculus essential oils and farnesene also inhibited human neutrophil chemotaxis induced by fMLF, suggesting these treatments down-regulated human neutrophil responses to inflammatory chemoattractants. Thus, our studies have identified farnesene as a potential anti-inflammatory modulator of human neutrophils.

show abstract

“…[ 72 ]. Although the α- and β-forms of farnesene are well-known pheromones [ 73 , 74 ], we did not find any reports on the biological activity of these compounds in human cells.…”

Section: Resultscontrasting

confidence: 66%

Neutrophil Immunomodulatory Activity of Farnesene, a Component of Artemisia dracunculus Essential Oils

Schepetkin

Özek

et al. 2022

Pharmaceuticals

View full text Add to dashboard Cite

show abstract

“…For example, OBP3, OBP4, OBP6, OBP7, OBP9, and OBP10 in Chrysopa pallens bind plant volatiles and aphid alarm pheromone EBF, and, OBP10 specifically binds EBF ( Li, et al, 2017 ; Li et al, 2019 ). The new OBPs from the aphid natural enemy Eupeodes corollae , OBP12, OBP15 and OBP16 also bound with EBF and plant volatiles, among which OBP12 and OBP15 strongly bound EBF ( Wang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

et al. 2022

View full text Add to dashboard Cite

In China, Aphidius gifuensis is one of the most common endoparasitoids of the green peach aphid Myzus persicae and grain aphid Sitobion miscanthi in the field. Insect odorant-binding proteins (OBPs) play vital roles in odor perception during feeding, host searching, mating and oviposition. In addition, some OBPs are involved in other physiological processes such as gustation and reproduction. In the present study, a comparative antennal transcriptomic analysis was applied between male and female A. gifuensis. The spatial expression patterns among antennae, heads, thoraxes, abdomens and legs of OBPs in both sexes were further profiled. Fifteen AgifOBPs were predicted, and 14 of them were identified by gene cloning, including 12 classic OBPs and 2 min-C OBPs. As expected, all OBPs were mainly expressed at high levels in antennae, heads or legs which are sensory organs and tissues. Finally, ligand binding properties of 2 OBPs (AgifOBP7 and AgifOBP9) were further evaluated. Female leg specifically expressed AgifOBP9 displays a broad and high binding property to aphid alarm pheromones, plant green volatiles and aphid sex pheromones (Ki < 10 μΜ). However, female leg specifically expressed AgifOBP7 displays poor affinity for all tested ligands except CAU-II-11 ((E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methoxybenzoate), a reported (E)-β-farnesene (EBF) analog with an exceptionally high binding affinity (Ki = 1.07 ± 0.08 μΜ). In summary, we reported the spatial expression pattern of the OBP repertoire in A. gifuensis, and further studied the binding properties of OBP7 and OBP9, which are mainly expressed in female legs, laying the foundation for the dissection of the contribution of OBPs to chemosensation in A. gifuensis.

show abstract

“…In addition, their relatively close relatedness to the fruit fly, Drosophila melanogaster , assists in orthology assignment for the prediction of gene function, while numerous genomes are available or in production which will greatly aid in future investigations into the molecular evolution of migratory traits (Hawkes & Wotton, 2021, 2022). Importantly, various hoverfly species can also be cultured in the lab and protocols and transgenic lines have been established to investigate gene function, making them a promising model to study the genetics of migration (Lemke et al, 2010; Wang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide transcriptomic changes reveal the genetic pathways involved in insect migration

et al. 2022

View full text Add to dashboard Cite

Insects are capable of extraordinary feats of long-distance movement that have profound impacts on the function of terrestrial ecosystems. The ability to undertake these movements arose multiple times through the evolution of a suite of traits that make up the migratory syndrome, however the underlying genetic pathways involved remain poorly understood. Migratory hoverflies (Diptera: Syrphidae) are an emerging model group for studies of migration. They undertake seasonal movements in huge numbers across large parts of the globe and are important pollinators, biological control agents and decomposers. Here, we assembled a high-quality draft genome of the marmalade hoverfly (Episyrphus balteatus). We leveraged this genomic resource to undertake a genome-wide transcriptomic comparison of actively migrating Episyrphus, captured from a high mountain pass as they flew south to overwinter, with the transcriptomes of summer forms which were non-migratory. We identified 1543 genes with very strong evidence for differential expression. Interrogation of this gene set reveals a remarkable range of roles in metabolism, muscle structure and function, hormonal regulation, immunity, stress resistance, flight and feeding behaviour, longevity, reproductive diapause and sensory perception. These features of the migrant phenotype have arisen by the integration and modification of pathways such as insulin signalling for diapause and longevity, JAK/SAT for immunity, and those leading to octopamine production and fuelling to boost flight capabilities. Our results provide a powerful genomic resource for future research, and paint a comprehensive picture of global expression changes in an actively migrating insect, identifying key genomic components involved in this important life-history strategy.

show abstract

Molecular basis of (E)-β-farnesene-mediated aphid location in the predator Eupeodes corollae

Cited by 44 publications

References 61 publications

Neutrophil Immunomodulatory Activity of Farnesene, a Component of Artemisia dracunculus Essential Oils

Neutrophil Immunomodulatory Activity of Farnesene, a Component of Artemisia dracunculus Essential Oils

Spatial Expression Analysis of Odorant Binding Proteins in Both Sexes of the Aphid Parasitoid Aphidius gifuensis and Their Ligand Binding Properties

Genome‐wide transcriptomic changes reveal the genetic pathways involved in insect migration

Contact Info

Product

Resources

About