Molecular basis of Cyperus difformis cross-resistance to ALS-inhibiting herbicides

Ntoanidou, Symela; Kaloumenos, Nikolaos S.; Diamantidis, Grigorios; Madesis, Panagiotis; Eleftherohorinos, Ilias G.

doi:10.1016/j.pestbp.2015.09.004

Cited by 22 publications

(15 citation statements)

References 32 publications

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“…A maioria dos casos registrados de resistência aos herbicidas inibidores da ALS é causada por alterações no local de ação da enzima, resultante de uma mutação pontual no gene da ALS (YU; POWLES, 2014). A mutação causa substituição de aminoácidos, alterando a estrutura da enzima e o local de ligação aos herbicidas (NTOANIDOU et al, 2016).…”

Section: Resultsunclassified

“…Mutações específicas no gene da enzima ALS, conferem elevada resistência aos grupos químicos das sulfonylureas, imidazolinones, triazolopyrimidines e pyrimidinyl(thio)benzoates (TRANEL et al, 2019), similar ao fator de resistência ≥ 10 do biótipo em questão (CHIAPINOTTO et al, 2017). As mutações evitam ou comprometem a ligação dos herbicidas à enzima ALS (NTOANIDOU et al, 2016). No entanto, os inibidores da ALS aplicados em associação com herbicidas que apresentam outro mecanismo de ação, como os inibidores do Fotossistema II (FS II), tanto em formulação comercial como o bentazon + imazamox ou em mistura como o [(bentazone + imazamox) + (imazapyr + imazapic)] e [bentazone + (imazapyr + imazapic)], foram eficientes no controle de ambos os biótipos de CYPIR-R e CYPIR-S.…”

Section: Resultsunclassified

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Alternativas de controle químico de junquinho resistente aos herbicidas inibidores da ALS

et al. 2019

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Cyperus iria L. (CYPIR) é uma das principais ciperáceas ocorrentes em lavouras de arroz irrigado. Durante muitos anos, os herbicidas inibidores da enzima acetolactato sintase (ALS) foram a principal ferramenta para controle de CYPIR, favorecendo a seleção de biótipos com resistência cruzada. Com base no nível de resistência, é necessária a adoção de medidas de controle químico alternativo a fim de evitar a evolução da resistência e reduzir a interferência sobre a cultura. O objetivo desse trabalho foi avaliar alternativas de controle químico de CYPIR com resistência cruzada aos herbicidas inibidores da ALS, ocorrente em lavouras de arroz irrigado no Sul do Brasil. Dois experimentos foram conduzidos em casa de vegetação, em delineamento inteiramente casualizado com quatro repetições. Os herbicidas utilizados foram: imazethapyr; formulação de imazapyr+imazapic; bentazon; propanil; formulação de bentazon+imazamox; mistura de (bentazone + imazamox) + (imazapyr + imazapic); associação de bentazone + formulação de (imazapyr + imazapic); glyphosate; e testemunha sem herbicida. A avaliação do controle foi realizada em biótipo resistente (CYPIR-R) e suscetível (CYPIR-S), e a fitotoxicidade avaliada nas cultivares Guri Inta CL, IRGA 424 RI e IRGA 409. Aos 28 dias após aplicação (DAA) foi avaliado o controle/ fitotoxicidade e a matéria seca da parte aérea (MSPA). Todos os tratamentos testados, com exceção do imazethapyr e formulação de imazapyr+imazapic isolados, foram eficientes. Há alternativas para o controle químico de Cyperus iria resistente aos inibidores da ALS e esses, quando associados com produtos que apresentam mecanismo de ação diferentes, não inviabilizam o uso da tecnologia ClearField®.

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Section: Resultsunclassified

Alternativas de controle químico de junquinho resistente aos herbicidas inibidores da ALS

et al. 2019

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“…In fact, most of the reported cases of resistance to these herbicides are due to a change in the enzyme resulting from a point mutation in the ALS gene. This mutation causes amino acid substitution, which alters the structure of the ALS enzyme and, consequently, its binding site to the herbicides (Ntoanidou et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Cross-Resistance of Rice Flatsedge to Als-Inhibiting Herbicides

et al. 2017

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-Cyperus iria (CYPIR) is one of the main weeds in irrigated rice crops. The intense chemical control with acetolactate synthase (ALS) inhibiting herbicides favors the selection of cross-resistance. This study aimed to evaluate the crossresistance of CYPIR to ALS-inhibiting herbicides in irrigated rice in Rio Grande do Sul. Two experiments arranged in a factorial scheme, in a completely randomized design, with four replications were conducted. Experiment I consisted of resistant (CYPIR-R) and susceptible (CYPIR-S) biotypes and ALS-inhibiting herbicide doses: imazethapyr (106 g a.i. ha ); corresponding to 0; 1/16; 1/8; 1/4; 1/2; 1; 2; 4; 8 and 16x (x = maximum registered dose). Experiment II followed the same procedures, with doses of 0; 1/64; 1/32; 1/16; 1/8; 1/4; 1/2; 1 and 2x for CYPIR-S and 0; 1/2; 1; 2; 4; 8; 16; 32; 64 and 128x for CYPIR-R; including bispyribac-sodium (50 g a.i. ha -1). The variables evaluated were: visual control at 7, 14, 21 and 28 days after the treatments (DAT) and shoot dry matter (SDM) at 28 DAT. The results, fitted by nonlinear regression, show varied and high levels of cross-resistance of CYPIR-R to ALS-inhibiting herbicides from the group of imidazolinones, sulfonylureas, triazolopyrimidines and pyrimidinyl(thio)benzoates. Bentazone can be used as an alternative herbicide, however, not exclusively in the control of this biotype.

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“…The amino acid residue 197 of the AHAS enzyme, which makes up the SU binding pocket, is located at the fifth-helix in the α domain of the AHAS catalytic subunit and involved in anchoring the aromatic ring through hydrophobic interactions [9,10]. In addition, the substitution of P197 in AHAS has been reported to result in herbicide resistance in A. thaliana [33], soybean [31] and many weeds [34,35]. In the present study, the transgenic BnAHAS1 544T Arabidopsis experiment revealed that the overexpression of the BnAHAS1 544T allele conferred TBM resistance (Figure 5a).…”

Section: Discussionmentioning

confidence: 99%

Inheritance and Molecular Characterization of a Novel Mutated AHAS Gene Responsible for the Resistance of AHAS-Inhibiting Herbicides in Rapeseed (Brassica napus L.)

Huang

Sun

et al. 2020

IJMS

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The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the acetohydroxyacid synthase (AHAS) gene can lead to AHAS-inhibiting herbicide resistance. In this study, the inheritance and molecular characterization of the tribenuron-methyl (TBM)-resistant rapeseed (Brassica napus L.) mutant, K5, are performed. Results indicated that TBM-resistance of K5 was controlled by one dominant allele at a single nuclear gene locus. The novel substitution of cytosine with thymine at position 544 in BnAHAS1 was identified in K5, leading to the alteration of proline with serine at position 182 in BnAHAS1. The TBM-resistance of K5 was approximately 100 times that of its wild-type ZS9, and K5 also showed cross-resistance to bensufuron-methyl and monosulfuron-ester sodium. The BnAHAS1544T transgenic Arabidopsis exhibited higher TBM-resistance than that of its wild-type, which confirmed that BnAHAS1544T was responsible for the herbicide resistance of K5. Simultaneously, an allele-specific marker was developed to quickly distinguish the heterozygous and homozygous mutated alleles BnAHAS1544T. In addition, a method for the fast screening of TBM-resistant plants at the cotyledon stage was developed. Our research identified and molecularly characterized one novel mutative AHAS allele in B. napus and laid a foundation for developing herbicide-resistant rapeseed cultivars.

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Molecular basis of Cyperus difformis cross-resistance to ALS-inhibiting herbicides

Cited by 22 publications

References 32 publications

Alternativas de controle químico de junquinho resistente aos herbicidas inibidores da ALS

Alternativas de controle químico de junquinho resistente aos herbicidas inibidores da ALS

Cross-Resistance of Rice Flatsedge to Als-Inhibiting Herbicides

Inheritance and Molecular Characterization of a Novel Mutated AHAS Gene Responsible for the Resistance of AHAS-Inhibiting Herbicides in Rapeseed (Brassica napus L.)

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