Molecular basis of bluetongue virus neutralization

Kahlon, Jasbir; Sugiyama, Kazuo; Roy, Polly

doi:10.1128/jvi.48.3.627-632.1983

Cited by 101 publications

(33 citation statements)

References 14 publications

(20 reference statements)

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“…BTV-10 VP2 protein, in excess of 50 ,ug per sheep, elicited neutralizing antibodies and totally protected the animals. These results confirmed previous observations that the outer capsid protein VP2 is the main determinant of the neutralization-specific immune response (1,10,11,13,14) and that it induces protection (11). Our data indicated that 50 ,ug of the expressed VP2 alone was insufficient to confer total protection.…”

Section: Discussionsupporting

confidence: 92%

“…Using immunoprecipitation techniques, Huismans and Erasmus (10) have shown that VP2 is a major serotype-specific antigen. This has been confirmed by analyzing intertypic reassortant viruses (14). Huismans and associates (11) demonstrated that VP2 polypeptide recovered from purified BTV induced neutralizing antibodies and protected sheep against virulent viral challenge, indicating the potential of using VP2 as a subunit vaccine.…”

mentioning

confidence: 80%

“…This core contains two major (VP3 and VP7) and three minor protein species (VP1, VP4, and VP6) and is surrounded by an outer capsid consisting of two major proteins, VP2 and VP5 (9, 18,32,33). It has been demonstrated both in vivo (using intertypic reassortment viruses) (14) and in vitro (by translation of each RNA segment) (19) that BTV RNA segment 2 codes for VP2. Using immunoprecipitation techniques, Huismans and Erasmus (10) have shown that VP2 is a major serotype-specific antigen.…”

mentioning

confidence: 99%

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Recombinant virus vaccine for bluetongue disease in sheep

Roy

Urakawa

Dijk

et al. 1990

J Virol

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119

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Bluetongue virus proteins derived from baculovirus expression vectors have been administered in different combinations to sheep, a vertebrate host susceptible to bluetongue virus, and the neutralizing antibody responses were measured. Vaccinated sheep were subsequently challenged, and the indices of clinical reaction were calculated. The results indicated that the outer capsid protein VP2 alone in doses of >50 ,ug per sheep elicited protection. A dose of ca. 50 ,ug of VP2 protected some but not all sheep. However, when used in combination with ca. 20 ,ug of the other outer capsid protein, VP5, 50-F,g quantities of VP2 not only protected all the vaccinated sheep but also elicited a higher neutralizing-antibody response. The addition of viral core proteins VP1, VP3, VP6, and VP7, the nonstructural proteins NS1, NS2, and NS3, and the outer capsid proteins VP2 and VP5 did not enhance this neutralizing-antibody response.

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Section: Discussionsupporting

confidence: 92%

mentioning

confidence: 80%

mentioning

confidence: 99%

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Recombinant virus vaccine for bluetongue disease in sheep

Roy

Urakawa

Dijk

et al. 1990

J Virol

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119

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“…The BTV genome encodes for seven structural (VP1 to VP7) and five non-structural proteins (NS1 to NS5) [2,3]. BTV serotype identification primarily relies on the serum neutralisation test, which is based on specific interactions between neutralising antibodies, generated during infection of the host, and the viral VP2 protein located on the outer capsid [4,5]. Based on the serum neutralisation test and/or phylogenetic analysis of VP2, which is the most variable protein, several novel BTV serotypes have been identified [6] in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Origin of Bluetongue Virus Serotype 8 Outbreak in Cyprus, September 2016

Rajko-Nenow

Christodoulou²,

Thurston

et al. 2020

Viruses

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In September 2016, clinical signs, indicative of bluetongue, were observed in sheep in Cyprus. Bluetongue virus serotype 8 (BTV-8) was detected in sheep, indicating the first incursion of this serotype into Cyprus. Following virus propagation, Nextera XT DNA libraries were sequenced on the MiSeq instrument. Full-genome sequences were obtained for five isolates CYP2016/01-05 and the percent of nucleotide sequence (% nt) identity between them ranged from 99.92% to 99.95%, which corresponded to a few (2–5) amino acid changes. Based on the complete coding sequence, the Israeli ISR2008/13 (98.42–98.45%) was recognised as the closest relative to CYP2016/01-05. However, the phylogenetic reconstruction of CYP2016/01-05 revealed that the possibility of reassortment in several segments: 4, 7, 9 and 10. Based on the available sequencing data, the incursion BTV-8 into Cyprus most likely occurred from the neighbouring countries (e.g., Israel, Lebanon, Syria, or Jordan), where multiple BTV serotypes were co-circulating rather than from Europe (e.g., France) where a single BTV-8 serotype was dominant. Supporting this hypothesis, atmospheric dispersion modelling identified wind-transport events during July–September that could have allowed the introduction of BTV-8 infected midges from Lebanon, Syria or Israel coastlines into the Larnaca region of Cyprus.

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“…BTV is a double‐capsid virus with a genome of 10 double‐stranded RNA segments encoding seven structural [viral protein 1 (VP1) to VP7] and five non‐structural proteins (Mertens et al., ; Ratinier et al., ). Serotype of BTV is recognized based on specific interactions of neutralizing antibodies (NA) with proteins displayed on the outer capsid of the virus (Huismans and Erasmus, ; Kahlon et al., ). VP2, protein of the outer capsid, is the most variable BTV protein and is considered the major serotype‐defining protein of BTV able to induce the production of neutralizing antibodies (NA) (Shaw et al., ).…”

Section: Introductionmentioning

confidence: 99%

Development of a Double-Antigen Microsphere Immunoassay for Simultaneous Group and Serotype Detection of Bluetongue Virus Antibodies

Bréard

Garnier

Desprès

et al. 2016

Transbound Emerg Dis

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Bluetongue viruses (BTV) are arboviruses responsible for infections in ruminants. The confirmation of BTV infections is based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs) using the BTV viral protein 7 (VP7) as antigen. The determination of the BTV serotype by serological analyses could be only performed by neutralization tests (VNT) which are time-consuming and require BSL3 facilities. VP2 protein is considered the major serotype-defining protein of BTV. To improve the serological characterization of BTV infections, the recombinant VP7 and BTV serotype 8 (BTV-8) VP2 were synthesized using insect cells expression system. The purified antigens were covalently bound to fluorescent beads and then assayed with 822 characterized ruminant sera from BTV vaccinations or infections in a duplex microsphere immunoassay (MIA). The revelation step of this serological duplex assay was performed with biotinylated antigens instead of antispecies conjugates to use it on different ruminant species. The results demonstrated that MIA detected the anti-VP7 antibodies with a high specificity as well as a competitive ELISA approved for BTV diagnosis, with a better efficiency for the early detection of the anti-VP7 antibodies. The VP2 MIA results showed that this technology is also an alternative to VNT for BTV diagnosis. Comparisons between the VP2 MIA and VNT results showed that VNT detects the anti-VP2 antibodies in an early stage and that the VP2 MIA is as specific as VNT. This novel immunoassay provides a platform for developing multiplex assays, in which the presence of antibodies against multiple BTV serotypes can be detected simultaneously.

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Molecular basis of bluetongue virus neutralization

Cited by 101 publications

References 14 publications

Recombinant virus vaccine for bluetongue disease in sheep

Recombinant virus vaccine for bluetongue disease in sheep

Origin of Bluetongue Virus Serotype 8 Outbreak in Cyprus, September 2016

Development of a Double-Antigen Microsphere Immunoassay for Simultaneous Group and Serotype Detection of Bluetongue Virus Antibodies

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