Molecular basis for transposase activation by a dedicated AAA+ ATPase
Álvaro de la Gándara,
Mercedes Spínola-Amilibia,
Lidia Araújo-Bazán
et al.
Abstract:Transposases drive chromosomal rearrangements and the dissemination of drug-resistance genes and toxins1–3. Although some transposases act alone, many rely on dedicated AAA+ ATPase subunits that regulate site selectivity and catalytic function through poorly understood mechanisms. Using IS21 as a model transposase system, we show how an ATPase regulator uses nucleotide-controlled assembly and DNA deformation to enable structure-based site selectivity, transposase recruitment, and activation and integration. So… Show more
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