Molecular Basis for Downregulation of C5a-Mediated Inflammation by IgG1 Immune Complexes in Allergy and Asthma

Pandey, Manoj Kumar

doi:10.1007/s11882-013-0387-3

Cited by 25 publications

(28 citation statements)

References 121 publications

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“…Furthermore, OVA-specific IgG2a levels were also significantly lower in Sensitized + Infected animals compared to Sensitized animals. The reduction in OVA-specific IgG was not subclass-dependent, as OVA-specific IgG1, an anti-inflammatory IgG subclass associated with tolerance (14), was also lower in Sensitized + Infected animals compared to the Sensitized group. (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex–Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice

Evans

Killoran

Mitre

et al. 2015

The Journal of Immunology

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In this study we evaluated the effect chronic helminth infection has on allergic disease in mice previously sensitized to ovalbumin (OVA). 10 weeks of infection with Litomosoides sigmodontis reduced immunological markers of type I hypersensitivity, including OVA-specific IgE, basophil activation, and mast cell degranulation. Despite these reductions, there was no protection against immediate clinical hypersensitivity following intradermal OVA challenge. However, late phase ear swelling, due to type III hypersensitivity, was significantly reduced in chronically infected animals. Levels of total IgG2a, OVA-specific IgG2a, and OVA-specific IgG1 were reduced in the setting of infection. These reductions were likely due to increased antibody catabolism as ELISPOT assays demonstrated that infected animals do not have suppressed antibody production. Ear histology 24 hours after challenge showed infected animals have reduced cellular infiltration in the ear, with significant decreases in numbers of neutrophils and macrophages. Consistent with this, infected animals had less neutrophil-specific chemokines CXCL-1 and CXCL-2 in the ear following challenge. Additionally, in vitro stimulation with immune-complexes resulted in significantly less CXCL-1 and CXCL-2 production by eosinophils from chronically infected mice. Expression of FcγRI was also significantly reduced on eosinophils from infected animals. These data indicate that chronic filarial infection suppresses eosinophilic responses to antibody-mediated activation and has the potential to be used as a therapeutic for pre-existing hypersensitivity diseases.

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Section: Resultsmentioning

confidence: 99%

Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex–Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice

Evans

Killoran

Mitre

et al. 2015

The Journal of Immunology

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“…What’s more, IgG1 is a subclass of IgG, which has a similar regulating effect as IgE, and is always deemed the surrogate marker for IgE. Hence IgE together with IgG1 are usually used to estimate the immunogenicity or adjuvant effects in the human body or in an animal model [ 35 ]. Therefore T-IgE, OVA-sIgE and OVA-sIgG1 were chosen as the immunological parameters to investigate the DEHP-adjuvant effect in this mouse asthma model.…”

Section: Discussionmentioning

confidence: 99%

Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model

You

Wei

et al. 2016

PLoS ONE

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Di-(2-ethylhexyl) phthalate (DEHP), a commonly used plasticizer, has an adjuvant effect in combination with ovalbumin (OVA). The adjuvant effect of DEHP has already been verified in our previous studies. In this study, to further investigate whether thymic stromal lymphopoietin (TSLP) was involved in the DEHP-adjuvant effect, DEHP was administered through a daily gavage exposure route. Mice were sensitized with ovalbumin (OVA) to trigger allergic responses, and an anti-TSLP monoclonal antibody was used to neutralize the effect of TSLP. Biomarkers including cytokines in bronchoalveolar lavage fluid (BALF), serum total IgE and TSLP content in the lung were detected. In addition, airway hyperreactivity and lung sections were examined. Collectively, these data indicated a salient Th2 response which was characterized by the upregulation of Th2-type cytokines, such as interleukin 4 (IL-4), IL-5 and IL-13. Moreover, the eosinophil number in BALF and the eosinophil cationic protein (ECP) in the lung were seen to have increased significantly. However, neutralization of TSLP with an anti-TSLP mAb reversed the adjuvant effect of DEHP on airway inflammation, structural alterations in the airway wall and increased airway hyperresponsiveness (AHR) to methacholine induced by the OVA allergen, suggesting that TSLP was an effective target site for suppressing the adjuvant effect of DEHP co-exposure.

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“…In humans, many insights have been made into the pathogenesis of diseases such as rheumatoid arthritis and glomerulonephritis, using the analysis of the levels of circulating immune complexes levels (Nowak-Łoś et al 2013, Navolotskaya 2014, Wener 2014. The incidence of circulating immune complexes has been ascertained for many disease conditions (Haymen 1990, Pandey 2013, Maheswari et al 2014) and the level of circulating immune complexes has been correlated with the activity of certain diseases. Moreover, the assays have also been used to evaluate the effect of pharmacological therapy on the levels of circulating immune complexes (Murunikkara and Rasool 2014).…”

Section: Discussionmentioning

confidence: 99%

Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

Niedźwiedź¹,

Jaworski²,

Kubiak³

2014

Polish Journal of Veterinary Sciences

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The aim of this study was to investigate the levels of circulating immune complexes (CICs) and concentration of haptoglobin, fibrinogen and C-reactive protein in the serum of horses with recurrent airway obstruction and healthy controls. The study was conducted on a group of 14 adult Polish Konik horses, kept in uniform environmental and living conditions. Horses were divided into two groups: 7 horses were not affected by any respiratory problem (control group) and 7 horses had a history of recurrent airway obstruction (RAO) (study group). A clinical and laboratory evaluation, endoscopic examination and bronchoalveolar lavage (BAL) were performed in all horses. Levels of circulating immune complexes were significantly (p=0.0057) increased in heaves-affected horses compared to healthy controls (median [25 th -75 th percentiles]) (3.96 [3.96 -4.43] vs. 7.46 [5.13 -11.9]). No significant difference was observed in the levels of the examined acute phase proteins between the groups. Moreover, all results were within the reference range established for horses. The results of this study indicate that heaves in horses is associated with the formation and high level of CICs. Haptoglobin, fibrinogen and C-reactive protein failed as markers of early stage systemic inflammation in the course of RAO.

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Molecular Basis for Downregulation of C5a-Mediated Inflammation by IgG1 Immune Complexes in Allergy and Asthma

Cited by 25 publications

References 121 publications

Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex–Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice

Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex–Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice

Thymic Stromal Lymphopoietin Neutralization Inhibits the Immune Adjuvant Effect of Di-(2-Ethylhexyl) Phthalate in Balb/c Mouse Asthma Model

Circulating immune complexes and markers of systemic inflammation in RAO-affected horses

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