Molecular basis for chemoprevention by sulforaphane: a comprehensive review

Juge, Nathalie; Mithen, Richard; Τράκα, Μαρία

doi:10.1007/s00018-007-6484-5

Cited by 628 publications

(515 citation statements)

References 170 publications

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“…These findings are commensurate with in vitro and in vivo studies where sulforaphane induced quinone reductase activity both in Hepa1c1c7 mouse hepatoma cell and in male Fisher 344 rats (Hintze et al, 2003). The upregulation of phase II enzymes by sulforaphane involves the antioxidant response element (ARE), the transcription of which is regulated by Nrf2 (Nuclear factor-erythroid 2 p45-related factor 2) (Juge et al, 2007); thus reflect the potential in preventing the Keap1-mediated degradation of Nrf2. However, glutathione S-transferase activity was not affected; discrepancy with Hanlon et al (2009b) though this may reflect in tissue differences.…”

Section: Discussionsupporting

confidence: 74%

Sulforaphane is Superior to Glucoraphanin in Modulating Carcinogen-Metabolising Enzymes in Hep G2 Cells

Razis

Noor²

2013

Asian Pacific Journal of Cancer Prevention

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Glucoraphanin is the main glucosinolate found in broccoli and other cruciferous vegetables (Brassicaceae). The objective of the study was to evaluate whether glucoraphanin and its breakdown product sulforaphane, are potent modulators of various phase I and phase II enzymes involved in carcinogen-metabolising enzyme systems in vitro. The glucosinolate glucoraphanin was isolated from cruciferous vegetables and exposed to human hepatoma cell line HepG2 at various concentrations (0-25 µM) for 24 hours. Glucoraphanin at higher concentration (25 µM) decreased dealkylation of methoxyresorufin, a marker for cytochrome P4501 activity; supplementation of the incubation medium with myrosinase (0.018 U), the enzyme that converts glucosinolate to its corresponding isothiocyanate, showed minimal induction in this enzyme activity at concentration 10 µM. Quinone reductase and glutathione S-transferase activities were unaffected by this glucosinolate; however, supplementation of the incubation medium with myrosinase elevated quinone reductase activity. It may be inferred that the breakdown product of glucoraphanin, in this case sulforaphane, is superior than its precursor in modulating carcinogenmetabolising enzyme systems in vitro and this is likely to impact on the chemopreventive activity linked to cruciferous vegetable consumption.

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Section: Discussionsupporting

confidence: 74%

Sulforaphane is Superior to Glucoraphanin in Modulating Carcinogen-Metabolising Enzymes in Hep G2 Cells

Razis

Noor²

2013

Asian Pacific Journal of Cancer Prevention

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“…For recent reviews please see [1][2][3]. The ability of ITCs to reduce carcinogen-induced tumourigenesis in experimental animals is relatively well understood; ITCs increase carcinogen detoxification via inhibition of carcinogen-activating phase I enzymes and induction of carcinogen-detoxifying phase II enzymes.…”

Section: Introductionmentioning

confidence: 99%

Anti-angiogenic effects of dietary isothiocyanates: Mechanisms of action and implications for human health

Cavell

Alwi

Donlevy

et al. 2011

Biochemical Pharmacology

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To cite this version:Breeze E. Cavell, Sharifah S. Syed Alwi, Alison Donlevy, Graham Packham. Anti-angiogenic effects of dietary isothiocyanates; mechanisms of action and implications for human health. Biochemical Pharmacology, Elsevier, 2010, 81 (3) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A c c e p t e d M a n u s c r i p t 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 Isothiocyanates and angiogenesis -3 - AbstractIsothiocyanates (ITCs) are electrophilic compounds derived from plants and are thought to play a major role in the potential chemopreventive effects associated with high intake of cruciferous vegetables. ITCs are also being evaluated for chemotherapeutic activity in early phase clinical trials. In addition to their effects on carcinogen metabolism and cancer cell survival and proliferation, ITCs have been shown to effectively interfere with angiogenesis in vitro and in vivo. Angiogenesis is the development of a new blood supply from existing vasculature and is required for tumours to develop beyond a small size limit determined by the diffusion limit for oxygen. Inhibition of angiogenesis may play a key role in the potential chemopreventive/chemotherapeutic activity of ITCs. In this review we highlight recent data demonstrating that ITCs have anti-angiogenic activity and identify potential molecular targets for these effects, including hypoxia-inducible factor (HIF), nuclear factor B (NF-B), activator protein 1 (AP1) and tubulin. We also discuss these findings in light of the potential chemopreventive/chemotherapeutic effects of ITCs.

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“…Beside a multitude of synthetic substances also numerous phytochemicals have been identified to exhibit potent chemopreventive effects in different carcinogenesis models while, at the same time showing low toxicity [6]. SFN is a naturally occuring isothiocyanate derived from cruciferous vegetables such as broccoli, cauliflower, cabbage and kale, which targets cancer initiation and progression both in vitro and in vivo, and further induces antiproliferative and cytotoxic effects in cells that are already transformed [7]. Recently, was SFN was identified as a novel histone deacetylase inhibitor (HDACi) in colon and prostate cancer cells [8].…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane potentiates oxaliplatin-induced cell growth inhibition in colorectal cancer cells via induction of different modes of cell death

Kaminski

Weigert

Brüne

et al. 2010

Cancer Chemother Pharmacol

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The objective of this study was to investigate, whether the plant-derived isothiocyanate Sulforaphane (SFN) enhances the anti-tumor activities of the chemotherapeutic agent oxaliplatin (Ox) in a cell culture model of colorectal cancer. Caco-2 and SW-620 cells were cultured under standard conditions and treated with increasing concentrations of SFN [1-20µM] and/or Ox [100nM-10µM]. For co-incubation, cells were pre-treated with SFN for 24 h. Cell growth was determined by BrdU incorporation. Drug interactions were assessed using the combination-index method (CI) (Cl<1 indicates synergism). Apoptotic events were characterized by different ELISA techniques. Protein levels were examined by Western blot analysis. Annexin-V-and propidium-iodide (PI)-staining followed by FACS analysis was 2 used to differentiate between apoptotic and necrotic events. SFN and Ox alone inhibited cell growth of Caco-2-and SW620-cells in a dose dependent manner, an effect, which could be synergistically enhanced, when cells were incubated with the combination o f both agents.Cotreated cells further displayed distinctive morphological changes that occurred during the apoptotic process, such as cell surface exposure of phosphatidylserine, membrane blebbing as well as the occurence of cytoplasmic histone-associated DNA fragments. Further observations thereby pointed towards simultaneous activation of both extrinsic and intrinsic apoptotic pathways. With increasing concentrations and treatment duration a shift from apoptotic to necrotic cell death could be observed. In conclusion, the data suggest that the isothiocyanate SFN sensitizes colon cancer cells to Ox-induced cell growth inhibition via induction of different modes of cell

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Molecular basis for chemoprevention by sulforaphane: a comprehensive review

Cited by 628 publications

References 170 publications

Sulforaphane is Superior to Glucoraphanin in Modulating Carcinogen-Metabolising Enzymes in Hep G2 Cells

Sulforaphane is Superior to Glucoraphanin in Modulating Carcinogen-Metabolising Enzymes in Hep G2 Cells

Anti-angiogenic effects of dietary isothiocyanates: Mechanisms of action and implications for human health

Sulforaphane potentiates oxaliplatin-induced cell growth inhibition in colorectal cancer cells via induction of different modes of cell death

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