Molecular Bases of Aerobic Bacterial Degradation of Dioxins: Involvement of Angular Dioxygenation

Nojiri, Hideaki; Omori, Toshio

doi:10.1271/bbb.66.2001

Cited by 90 publications

(75 citation statements)

References 61 publications

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“…Although bioremediation techniques for dioxin-polluted sites have remained undeveloped, basic information on the mechanism of microbial dioxin degradation as well as on the biodiversity and ecophysiology of dioxin-degrading microorganisms has accumulated, particularly during the past decade (for reviews, refs. 9, 23,133,136,198). The available information indicates that the ring structures of dioxins and dioxin-like compounds are degraded by aerobic bacteria that contain aromatic hydrocarbon dioxygenases having a broad substrate specificity.…”

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confidence: 99%

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Hiraishi

2003

Microb. Environ.

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Dioxins are a group of chloroaromatic compounds known to be toxic and carcinogenic, and persistent environmental pollutants. How to remedy dioxin-polluted environments is one of the most challenging problems in environmental technology. From ecological and economical viewpoints, biological methods using particular microorganisms and microbial consortia capable of dioxin transformation and degradation have greater appeal than physicochemical methods in their application for environmental remediation. Large numbers of microorganisms capable of degrading dioxins and dioxin-like compounds have been isolated and characterized. Information about the biodiversity, ecophysiology and molecular biology of dioxin-degrading microorganisms has accumulated particularly during the past decade. There are three major modes of microbial degradation and transformation of dioxins; that is, oxidative degradation by aerobic bacteria containing aromatic hydrocarbon dioxygenases, reductive dechlorination by anaerobic microorganisms and fungal oxidation with cytochrome P-450, lignin peroxidases and laccases. This article overviews the current knowledge of microbial degradation and transformation of dioxins as well as the biodiversity of microorganisms involved. Strategies directed towards the bioremediation of dioxin-polluted environments are discussed.

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confidence: 99%

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Hiraishi

2003

Microb. Environ.

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“…Most of the evidence for aerobic biodegradation of PCDD/Fs has been obtained with lower chlorinated dioxins (Field and Sierra-Alvarez, 2008). Aerobic bacteria from the genera of Sphingomonas, Pseudomonas and Burkholderia have aromatic ring hydroxylating dioxygenase enzymes, but they are usually only able to degrade mono-, di-and trichloro-dibenzo-p-dioxins and -furans (Habe et al, 2001;Nojiri and Omori, 2002) (Figure 5). One exception is the Sphingomonas wittichii strain RW1, which was shown to transform also higher chlorinated dioxins (Nam et al, 2006).…”

Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

“…In addition, 2,3-DCDD and 2,3,7-TriCDD have been degraded with a bacterial P450 from Bacillus megaterium (Sulistyaningdyah et al, 2004). (Bunge et al, 2003), b) 2-CDD by aerobic bacteria (angular dioxygenase) (Habe et al, 2001;Nojiri and Omori, 2002), c) 2,7-DCDD by Phanerochaete chrysosporium (lignin peroxidase) .…”

Section: Biodegradation Of Pcdd/fsmentioning

confidence: 99%

Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

et al. 2014

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Soils that are contaminated with the most recalcitrant organic contaminants, such as high molecular weight polyaromatic hydrocarbons (HMW PAH) and polychlorinated dibenzo-pdioxins and dibenzofurans (PCDD/F), cannot be degraded efficiently by conventional composting. The only available treatment method for these soils, which destroys the contaminants, is combustion at high temperature. This thesis examines three alternative fungal methods to treat these soils: 1) treatment with fungal enzymes, 2) treatment with fungal inoculum, and 3) fungal treatment used as a pre-treatment to improve the energy efficacy in combustion. Manganese peroxidase (MnP), which belongs to lignin-modifying enzymes (LME), was produced and used to treat PCDD/F-contaminated soil in the laboratory scale. Nevertheless, no degradation with a MnP preparation was observed, although a substantial amount of MnP activity was found in the soil still after 10 days of incubation. Both PAH-and PCDD/Fcontaminated soils were treated with fungal inoculum in the laboratory scale. HMW PAHs were degraded significantly more by the fungi than by the indigenous microbes alone in the laboratory experiments, where the PAH concentration of soil was 3500 mg kg -1 (sum of 16 PAH). Treatment with Phanerochaete velutina (inoculum) resulted to degradation of 96 % of 4-ring PAHs and 39 % of 5-and 6-ring PAHs in three months. With PCDD/F-contaminated soil, no degradation was observed in the control, but the degradation of PCDD/Fs with fungal treatments was significant (P. velutina: 62 %, Stropharia rugosoannulata: 64 % of WHO-TEQ value). Fungal treatment of PAH-contaminated soil was also applied in the field scale (2 t). However, both P. velutina (inoculum) and control treatment resulted in equal degradation in soil with lower PAH concentration (1400 mg kg -1 , sum of 16 PAH): 94 % of the 16 PAHs were degraded in three months. Fungal treatment was even applied as a pre-treatment for contaminated soil with high organic matter content, and which will be later combusted. In the pilot-scale (300 kg), 13 % degradation of the original organic matter content was obtained in 6 months. To conclude, fungal treatment is reasonable to apply for soils with organic contaminants that cannot be bioremediated by composting. With soils contaminated by chlorinated dioxins, this is always the case, but also PAH-contaminated soils with high total concentration or high proportion of HMW-PAHs. In addition, with fungal treatment the amount of organic matter in the soil can be reduced and the efficacy of the combustion process is improved. Tiivistelmä Kaikkein vaikeimmin hajoavilla orgaanisilla yhdisteillä kuten monirenkaisilla polyaromaattisilla hiilivedyillä (PAH) sekä polyklooratuilla dibenzo-p-dioksiineilla ja -furaaneilla (PCDD/F) pilaantuneita maita ei pystytä tehokkaasti kunnostamaan perinteisellä kompostoinnilla. Ainoa käytössä oleva puhdistusmenetelmä, joka hajottaa pilaavat yhdisteet, on maan poltto korkeassa lämpötilassa. Tämä väitöskirja esittelee kolme vaihtoehtoista valkolah...

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“…In addition, in a culture medium of CA10 grown on carbazole, the production of 2 0 -aminobiphenyl-2,3-diol and its meta-cleavage product, 2-hydroxy-6-oxo-6-(2 0 -aminophenyl)-hexa-2,4-dienoate (HOADA), was probable. Based on an analogy of the metabolic intermediates of carbazole with those in the dibenzofuran and fluorene degradation pathways, [5][6][7][8] a carbazole degradation pathway was proposed (Fig. 1).…”

Section: Angular Dioxygenation: a Novel Reaction Involved In Carbamentioning

confidence: 99%

Molecular Bases of Aerobic Bacterial Degradation of Dioxins: Involvement of Angular Dioxygenation

Cited by 90 publications

References 61 publications

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Biodiversity of Dioxin-Degrading Microorganisms and Potential Utilization in Bioremediation.

Fungal enzyme production and biodegradation of polychlorinated dibenzo-p-dioxins and dibenzofurans in contaminated sawmill soil

Structural and Molecular Genetic Analyses of the Bacterial Carbazole Degradation System

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