Molecular authentication of Panax ginseng and ginseng products using robust SNP markers in ribosomal external transcribed spacer region

Rapidly discriminate commercial medicinal Pulsatilla chinensis (Bge.) Regel from its adulterants using ITS2 barcoding and specific PCR-RFLP assay

“…These barcoding systems provide open barcode databases and standard protocols for species identification. In recent years, a lot of quick identification methods based on DNA barcode markers were developed, such as Allele-specific PCR18, Real-time PCR19, High Resolution Melting (HRM) analysis20, PCR-RFLP21, and etc. These methods have simpler test procedures and shorter time-consuming than conventional methods that were more convenient for rapid identification.…”

Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker

“…It is clear that compounds within herbal medicines present chemical complexity, which are usually affected by growth, drying, and storage conditions, as well as the time of collection. Therefore, techniques involving molecular markers are more dependable as they are dependent on genetic composition and are not influenced by other factors (Wang et al, 2011b;Wu et al, 2011). RAPD analysis could be used to explore the high degree of genetic polymorphisms in various herbal medicines; not only is there no requirement for prior DNA sequence information of these species, but this technique is also easy to perform.…”

“…Genetic polymorphisms in medicinal plants have been widely studied, and have helped to differentiate herbal medicines at the genomic level. Various molecular markers have been explored for use in the authentication of botanical materials such as ginseng (Wang et al, 2011b), atractylodes (Mizukami et al, 2000), and such techniques can provide more objective and reliable tools for the categorization and authentication of plant materials. Conventionally, randomly amplified polymorphic DNA (RAPD), based on polymerase chain reaction (PCR) amplification, has been used to estimate genetic diversity in wild plant populations or cultivars.…”

Codon Usage Study on Chloroplast Genome in Medicinal Plant <i>Panax Ginseng</i>

“…Wan-jing Liu, et al, studied the HMGS and HMGR Genes from Panax notoginseng via bioinformatics tools [21]. Hongtao Wang, et al, explored the methods of authenticating the Panax ginseng and ginseng products via robust SNP markers [22]. Erin M. Schlag, et al, put forward a method for studying the relationship between genetic and chemotypic diversity in ginseng [23].…”