Molecular assessment of the transplacental transmission of Toxoplasma gondii , Neospora caninum , Brucella canis and Ehrlichia canis in dogs

Taques, Ísis Indaiara Gonçalves Granjeiro; Barbosa, Tatiane Rodrigues; Martini, Andresa de Cássia; Pitchenin, Letícia Camara; Braga, Ísis Assis; Melo, Andréia Lima Tomé; Nakazato, Luciano; Dutra, Valéria; Aguiar, Daniel Moura de

doi:10.1016/j.cimid.2016.09.002

Cited by 21 publications

(12 citation statements)

References 30 publications

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“…Diagnosis of B . canis infection remains a challenge due to frequent false negative results in direct and indirect diagnostic methods employed to detect the infection in adult dogs and humans 6 , 21 , 22 .…”

Section: Introductionmentioning

confidence: 99%

Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

Souza

Carvalho

Mol

et al. 2018

Sci Rep

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Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.

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Section: Introductionmentioning

confidence: 99%

Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

Souza

Carvalho

Mol

et al. 2018

Sci Rep

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“…However, in some studies such an association was not found JESUS et al, 2006;PLUGGE et al, 2011). Vertical transmission was also studied (TAQUES et al, 2016), with 41 stillborn puppies from 23 bitches. By PCR and IFAT, five (21.7%) bitches were positive and 22 (53.6%) stillborn were positive by PCR, utilizing ITS-1 DNA region/locus, being 17 from positive bitches and five from negative ones.…”

Section: Epidemiologymentioning

confidence: 99%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

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Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

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“…Vertical transmission from dam to puppies was not found to occur for Anaplasma phagocytophilum , based on a single case report, but it has been reported in 1 human case and in naturally and experimentally infected cows . Diagnostic support for vertical transmission of Ehrlichia canis was not found in 12 infected or exposed dams and their puppies, although that single study does not definitively rule out the possibility that transplacental transmission occurs. The potential role for vertical transmission in many other CVBDs, including hemotropic Mycoplasma spp.…”

Section: Introductionmentioning

confidence: 91%

“…of Ehrlichia canis was not found in 12 infected or exposed dams and their puppies, 26 although that single study does not definitively rule out the possibility that transplacental transmission occurs. The potential role for vertical transmission in many other CVBDs, including hemotropic Mycoplasma spp.…”

mentioning

confidence: 90%

Evidence for vertical transmission of Mycoplasma haemocanis, but not Ehrlichia ewingii, in a dog

Lashnits

Grant²,

Thomas

et al. 2019

Veterinary Internal Medicne

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A 2‐year‐old female intact pregnant Beagle was evaluated after the owner surrendered her to a shelter. Prepartum and 2 months postpartum at the time of routine spay, the dam was whole‐blood polymerase chain reaction (PCR) positive for Ehrlichia ewingii . She was also whole‐blood PCR positive for Mycoplasma haemocanis prepartum and continuously for 5 months thereafter. The dam delivered 5 healthy puppies, 1 of which was whole‐blood PCR positive for M. haemocanis . All 5 puppies had antibodies against Ehrlichia spp. at 1 month of age but not thereafter, and all puppies were Ehrlichia spp. PCR negative for 5 months of follow‐up. Therefore, this study supports a potential role for vertical transmission in the maintenance of M. haemocanis in dogs as reservoir hosts. In contrast, in this case there was no evidence that E. ewingii was transmitted transplacentally or during the perinatal period.

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Molecular assessment of the transplacental transmission of Toxoplasma gondii , Neospora caninum , Brucella canis and Ehrlichia canis in dogs

Cited by 21 publications

References 30 publications

Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

All about neosporosis in Brazil

Evidence for vertical transmission of Mycoplasma haemocanis, but not Ehrlichia ewingii, in a dog

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