Molecular Assessment of Some Tomato (Lycopersicon esculentum Mill) Genotypes Revealed by SCoT Markers

Abstract

The SCoT marker was able to shed light on the origin-specific genetic link between the genotypes under study, providing breeders with a valuable resource. Some Tomato (Lycopersicon esculentum Mill) genotypes were analyzed for their genetic diversity, genetic connection, and DNA fingerprint using ten molecular markers of the SCoT (Start Codon Targeted) type. Different SCoT primer combinations generated distinctive DNA fingerprints. The results demonstrate that polymorphism is most prevalent when using the primer SCoT30 (100 percent) and least prevalent when using the primer SCoT54 (50 percent) (33.3). This study found that the genetic distance between Bushra and Warda was the lowest (0.1111) and the genetic distance between Fouton and the Special Pack was the highest (0.55583), but that the distance between the two was the smallest (0.1111). (Special pack and Cherry tomato). Through the use of cluster analysis (a phylogenetic tree), the examined genotypes were divided into two distinct groups. The goals of this research were to use SCoT to catalog the variety of tomato genotypes and to discover the connections between the various molecular fingerprinting-based clustering methods.