Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques

Ivanović, Žarko; Popović, Tatjana; Janse, J.; Kojić, Milan; Stanković, Slaviša; Gavrilović, Veljko; Fira, Djordje

doi:10.1007/s10658-014-0531-5

Cited by 13 publications

(16 citation statements)

References 21 publications

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“…In Serbia, bacterial blight of walnut caused by X. a. pv. juglandis was first reported by Gavrilović and Arsenijević (1998) and was later characterised by Ivanović et al (2015). Deep bark canker (DBC) and shallow bark canker (SBC) caused by species of the genus Brenneria (rubrifaciens and nigrifluens, respectively) are other bacterial diseases that are able to affect walnut production (McClean et al, 2008;Frutos, 2010;Biosca & López, 2012;Popović et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Etiology of bacterial diseases of young walnut trees in Serbia

Iličić

Bagi

Blagojevic³

et al. 2021

PESTIC PHYTOMED

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In the summer and autumn of 2019-2020, young walnut orchards were monitored for the presence of bacterial diseases. Diseased walnut samples comprising trunks and branches with symptoms of vertical oozing canker (VOC), walnut bacterial blight (WBB) and superficial bark necrosis were collected from eight locations in Serbia. Based on phenotypic features, pathogenicity, and molecular assays using PCR with specific primers, 49 isolates obtained from samples showing VOC and WBB symptoms were identified as Xanthomonas arboricola pv. juglandis, while further two isolates obtained from bark necrosis were identified as Brenneria rubrifaciens. One tested X. a. pv. juglandis isolate obtained from a VOC sample produced deep cankers in the bark of inoculated trunks of young walnut trees (cultivars Chandler, Franquette and Sejnovo). Therefore, this is the first report of an association between X. a. pv. juglandis and VOC symptom in Serbia. Considering that X. a. pv. juglandis significantly endangers walnut production, the presence of this pathogen in walnut transplant imports needs to be assessed by an authorised laboratory. Furthermore, as this is also the first report of B. rubrifaciens on walnut trees in Serbia, it is noteworthy that this pathogen is not particularly harmful to young walnut trees.

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Section: Introductionmentioning

confidence: 99%

Etiology of bacterial diseases of young walnut trees in Serbia

Iličić

Bagi

Blagojevic³

et al. 2021

PESTIC PHYTOMED

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“…Walnut tree is acknowledged as a host plant species for X. arboricola, providing a permanent niche for the pathovar juglandis, which is a walnut pathogen characterized by a high genetic diversity (Giovanardi et al, 2015;Hajri et al, 2010;Ivanović et al, 2014;Kałużna et al, 2014). However, most of the Xaj genetic diversity studies are based on a small number of isolates, obtained during a short time period of one growing season, centred on specific walnut orchards and a narrow geographic territory (Giovanardi et al, 2015;Hajri et al, 2010;Ivanović et al, 2014;Kałużna et al, 2014;Marcelletti et al, 2010). These studies imply clonal population structure, which is often acknowledged in plant pathology (Lamichhane & Venturi, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…arboricola on walnut hosts (Essakhi et al, 2015; Merda et al, 2016), described as phylogenetically heterogeneous and grouping separately from the well‐defined clusters of pathogenic Xaj strains (Essakhi et al, 2015). Assessment of Xaj in France (Hajri et al, 2012), Italy (Giovanardi et al, 2015), and Serbia (Ivanović et al, 2014) showed large genetic diversity among isolates that, coupled with phenotypic features and geographic distribution, were consistent with distinct Xaj populations, according to these authors. Furthermore, the genetic differences found between Xaj strains collected from VOC and WBB symptoms suggests the presence of distinct genetic lineages within Xaj populations (Hajri et al, 2010).…”

Section: Introductionmentioning

confidence: 86%

“…Colours represent the country origin of the strains/isolates [Colour figure can be viewed at wileyonlinelibrary.com] (Parkinson et al, 2007;Young et al, 2008). In fact, the large number of sequences available in the GenBank database, the discriminatory power of each of these housekeeping genes for strains from pathovar juglandis, and its wide use in transversal studies on Xaj (Giovanardi et al, 2015;Ivanović et al, 2014;Kałużna et al, 2014;Marcelletti et al, 2010) makes MLSA a suitable method to rapidly infer diversity and identify deeply branching clusters within bacterial populations (Gevers et al, 2005). Furthermore, MLSA has been particularly useful for differentiating X. arboricola isolates obtained from the same host plant (Essakhi et al, 2015;Garita-Cambronero et al, 2017).…”

Section: To Comprehensively Characterize the Population Diversity Ofmentioning

confidence: 99%

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Comprehensive diversity assessment of walnut‐associated xanthomonads reveal the occurrence of distinct Xanthomonas arboricola lineages and of a new species (Xanthomonas euroxanthea) within the same tree

et al. 2021

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Xanthomonas arboricola pv. juglandis (Xaj) is the aetiological agent of walnut diseases causing economic losses on walnut production worldwide. This phytopathogen is spread around the world where walnuts are produced and has a considerable genetic diversity. Using a comprehensive sampling methodology, focusing on factors that could influence the diversity of walnut‐colonizing Xaj in Portugal, this work provides new insights on xanthomonad populations on walnut. Genetic diversity was assessed by multilocus sequence analysis (MLSA) and dot blot hybridization patterns on 131 Xanthomonas isolates obtained from 64 walnut trees considering epidemiological metadata such as year of isolation, distinct bioclimatic regions, production regimes, and host‐related features. The results showed that the majority of isolates were split into 17 lineages of Xaj, while the other isolates clustered in four MLSA groups that did not include Xaj strains. These four groups were represented by three lineages of X. arboricola, and 11 lineages of Xanthomonas spp., including strains assigned to the recently proposed new species Xanthomonas euroxanthea. Furthermore, distinct Xaj, X. arboricola, and Xanthomonas spp. were isolated from the same walnut tree, suggesting possible genetic admixture within the same host. Phylogenetic analysis through geoBurst revealed the high diversity of these Xanthomonas spp. populations. Assessment of type III effector genes gave the indication that some Xanthomonas spp. strains were nonpathogenic on walnut, with the exception for X. euroxanthea CPBF 424. Altogether, these findings add to the thorough characterization of walnut‐associated xanthomonads in Portugal, providing a comprehensive snapshot of the current diversity that could contribute to risk assessment analysis and improve phytosanitary control.

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“…Compared to other X. arboricola pathovars, X. arboricola pv. juglandis is characterized by a larger genetic diversity recorded throughout geographically distinct walnut cultivation regions [ 15 , 16 , 17 , 18 , 19 ]. Genotyping and population studies of walnut-associated xanthomonads identified a high diversity of X. arboricola pv.…”

Section: Introductionmentioning

confidence: 99%

Comparative Genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis Strains Isolated from a Single Walnut Host Tree

et al. 2021

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The recent report of distinct Xanthomonas lineages of Xanthomonas arboricola pv. juglandis and Xanthomonas euroxanthea within the same walnut tree revealed that this consortium of walnut-associated Xanthomonas includes both pathogenic and nonpathogenic strains. As the implications of this co-colonization are still poorly understood, in order to unveil niche-specific adaptations, the genomes of three X. euroxanthea strains (CPBF 367, CPBF 424T, and CPBF 426) and of an X. arboricola pv. juglandis strain (CPBF 427) isolated from a single walnut tree in Loures (Portugal) were sequenced with two different technologies, Illumina and Nanopore, to provide consistent single scaffold chromosomal sequences. General genomic features showed that CPBF 427 has a genome similar to other X. arboricola pv. juglandis strains, regarding its size, number, and content of CDSs, while X. euroxanthea strains show a reduction regarding these features comparatively to X. arboricola pv. juglandis strains. Whole genome comparisons revealed remarkable genomic differences between X. arboricola pv. juglandis and X. euroxanthea strains, which translates into different pathogenicity and virulence features, namely regarding type 3 secretion system and its effectors and other secretory systems, chemotaxis-related proteins, and extracellular enzymes. Altogether, the distinct genomic repertoire of X. euroxanthea may be particularly useful to address pathogenicity emergence and evolution in walnut-associated Xanthomonas.

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Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques

Cited by 13 publications

References 21 publications

Etiology of bacterial diseases of young walnut trees in Serbia

Etiology of bacterial diseases of young walnut trees in Serbia

Comprehensive diversity assessment of walnut‐associated xanthomonads reveal the occurrence of distinct Xanthomonas arboricola lineages and of a new species (Xanthomonas euroxanthea) within the same tree

Comparative Genomics of Xanthomonas euroxanthea and Xanthomonas arboricola pv. juglandis Strains Isolated from a Single Walnut Host Tree

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