Molecular Assessment of Anaplasma marginale in Bovine and Rhipicephalus (Boophilus) microplus Tick of Endemic Tribal Belt of Coastal South Gujarat, India

Kumar, Niranjan; Solanki, J. B.; Varghese, Anju; Jadav, Mehul M.; Das, Bhupamani; Patel, M. D.; Patel, D. R.

doi:10.2478/s11686-019-00041-z

Cited by 17 publications

(11 citation statements)

References 31 publications

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“…This high prevalence of buffalo positive for infection with rickettsial organisms was similar to those observed previously in Cuba (52% [20]) and Mozambique (72.2% [21]), but was higher than those reported in the Philippines (29% [22]), Malaysia (21.8% [23]), India (18.33% [24]), South Africa (17.3% [25]), Pakistan (14.73% [17]), Columbia (13.1% [26]), and northeast Thailand (8% [11]). In this study, the highest anaplasmosis prevalence was found in Southern Thailand (Phatthalung), while the lowest was at Northeastern Thailand (Nong Bua Lamphu).…”

Section: Discussionsupporting

confidence: 89%

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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Background Anaplasmosis, an animal disease caused by rickettsial bacteria in the genus Anaplasma, is of considerable economic importance in livestock animals in many countries worldwide. The objectives of this study were to determine the identity, prevalence, and geographic distribution of Ehrlichia and Anaplasma in naturally infected water buffalo in Thailand using PCR amplification and sequencing of the 16S ribosomal RNA and heat shock protein groEL genes. A total of 456 buffalo blood samples from Thailand were investigated. Species identification and genetic differentiation of intra-population and inter-population with the global isolates were conducted based on nucleotide sequences. Interplay between the infection and host factors was also assessed. Results Overall, 41% of water buffalo were found to be infected with rickettsial organisms in the family Anaplasmataceae, but Ehrlichia spp., Neorickettsia spp., and Wolbachia spp. were not found in any of the sequenced samples in this study. Female buffalo were more frequently infected with bacteria in the family Anaplasmataceae than males [71 out of 176 females (40.3%) versus 11 out of 47 males (23.4%)]. The Odds Ratio value indicated that the risk of infection for female buffalo was 2.2-fold higher than that for males (p < 0.05). We detected three haplotypes of A. marginale 16S rRNA gene and they were placed in a clade that was closely related to the A. marginale in buffalo in China; and cattle in Thailand, Uganda, and China. Homology searching of groEL sequences against the GenBank™ database using the BLASTn algorithm revealed that the obtained sequences had a high percentage similarity (98.36–99.62%) to A. platys sequences. The groEL sequences of three A. platys-like isolates were clustered in the same clade as the A. platys from the tick Rhipicephalus microplus in China. Conclusions Our data showed that the apparently healthy buffalo were naturally infected by bacteria in the family Anaplasmataceae at a relatively high prevalence. We also report the finding of A. platys-like infections in water buffalo in Thailand for the first time. Water buffalo serving as the reservoir host of anaplasmosis is of concern for managing the disease control and prevention in ruminants.

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Section: Discussionsupporting

confidence: 89%

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

2020

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“…Infections with Rhipicephalus (Boophilus) sp., Hyalomma sp., and Haemaphysalis sp., as well as mixed infections, were found to be 32.25 percent, 12.58 percent, 3.22 percent, and 6.12 percent, respectively [21]. This study's ndings were consistent with previous reports from various parts of India and abroad [22,23,24].…”

Section: Resultssupporting

confidence: 91%

Comparison of Molecular and Morphological Identification of Ticks in Vellore District of Tamil Nadu

Saravanan

Rajasekar

Prakash

et al. 2022

Preprint

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In this study, we have comparatively identified molecular and morphological features of ticks in Vellore district of Tamil Nadu. A total of 541 ticks were collected from domestic animals in the Vellore district of Tamil Nadu. The research was carried out between October 2018 and December 2019. The collected tick specimens were transferred into two sterile tubes and stored at room temperature in 70% alcohol. The tick was identified using PCR amplification of the 18SrRNA genes of Rhipicephalus sp (MW131877, MW318996, MW131878, MT952071) and Heamaphysalis sp (MW327046), the mtCOX1 gene of Rhipicephalus microplus (MT941478, MT941466, MT941463), and Hylomma marginatum (MW327046) (MW322564). The phylogenetic tree indicates that there was a common ancestor tick between Rhipicephalus sp., Heamaphysalis sp., and Hylomma marginatum in Vellore, Tamil Nadu. The tick was identified morphologically as two genera, Rhipicephalus sp. and Heamaphysalis sp. This is one of the first reports on ticks in the Vellore district of Tamil Nadu, as well as the morphological confirmation of two genera, Rhipicephalus and Heamaphisalis, through the sequencing of the 18SrRNA and mtCOX1 genes.

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“…A. marginale has previously been reported in Rh. microplus in Côte d'Ivoire [60], Ecuador [77], India [78] and Pakistan [79]. As for genus Ehrlichia, we recorded for the first time two different genotypes of "E. rustica" in both Rh.…”

Section: Discussionmentioning

confidence: 86%

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

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Molecular Assessment of Anaplasma marginale in Bovine and Rhipicephalus (Boophilus) microplus Tick of Endemic Tribal Belt of Coastal South Gujarat, India

Cited by 17 publications

References 31 publications

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Molecular detection and genetic characterization of Anaplasma marginale and Anaplasma platys-like (Rickettsiales: Anaplasmataceae) in water buffalo from eight provinces of Thailand

Comparison of Molecular and Morphological Identification of Ticks in Vellore District of Tamil Nadu

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

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