Molecular assays reveal the presence of Theileria spp. and Babesia spp. in Asian water buffaloes (Bubalus bubalis, Linnaeus, 1758) in the Amazon region of Brazil

Silveira, Júlia A.G.; Oliveira, Cairo Henrique Sousa de; Silvestre, Bruna T.; Albernaz, Tatiana T.; Leite, Rômulo Cerqueira; Barbosa, José Diomedes; Oliveira, Carlos Magno Chaves; Ribeiro, Múcio Flávio Barbosa

doi:10.1016/j.ttbdis.2016.05.009

Cited by 15 publications

(8 citation statements)

References 25 publications

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“…The results reported herein demonstrate that in the study area, the two Babesia species exhibited differences in prevalence between the mammalian hosts studied, with B. bigemina being most prevalent in cattle (24.2%) and B. bovis in buffaloes (17.7%). Similar results were observed in livestock areas from Argentina, Brazil and Colombia, in which the molecular prevalence of B. bigemina and B. bovis in cattle was 46.4 ± 7.0% and 11.7 ± 3.6%, respectively [ 10 , 18 , 38 – 40 ], while in buffaloes it was 8.6 ± 7.6% and 17.5 ± 8.8% for each species, respectively [ 41 – 43 ]. These findings, together with those obtained from the spatio-temporal analysis, in which these proportions did not vary with time (Fig.…”

Section: Discussionsupporting

confidence: 76%

Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

et al. 2018

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BackgroundBabesia bigemina and B. bovis are two economically important hemoparasites affecting both cattle and buffaloes involved in dairy and beef production. In Colombia, although some parasitological and serological studies suggest an endemicity of these pathogens in areas under 1000 m, little is known about its molecular prevalence in different host. The objective of this study was to estimate the prevalence and molecular traits of these parasites in cattle and buffaloes from two Colombian regions.MethodsBetween 2014 and 2016, a three-point longitudinal survey was designed in farms from Caribbean and Orinoquia regions to evaluate the molecular prevalence of B. bigemina and B. bovis using a nested PCR (n-PCR) targeting hypothetical protein (hyp) and rhoptry-associated protein (RAP-1) genes, respectively. A total of 1432 cattle, 152 buffalo and 1439 Rhipicephalus microplus samples were analyzed. Moreover, phylogenetic relationship of isolates was analyzed using the 18S rRNA gene.ResultsA molecular prevalence of 31.6% (24.2% for B. bigemina and 14.4% for B. bovis), 23.6% (6.5% for B. bigemina and 17.7% for B. bovis) and 4.3% (3.5% for B. bigemina and 1.0% for B. bovis) was observed in cattle, buffaloes and Rhipicephalus microplus, respectively. Higher values of infection were observed during the wet season and late wet season; nevertheless, other variables such as age, production type, sex, breed and babesiosis control were also significantly associated with infection. Prevalence analysis showed that B. bovis infection was higher in cattle that coexist with buffaloes, when compared to those which did not. For each species, phylogenetic analyses revealed a high genetic diversity of isolates without clusters related to the isolation source.ConclusionsTo our knowledge, this is the first longitudinal survey that evaluates through molecular methods, the infection of B. bigemina and B. bovis in two important livestock regions from Colombia. This study reveals that the prevalence of infection by Babesia spp., in cattle and buffaloes are modulated by seasonal variations, host factors and vector traits. Our results provide new insights on the epidemiological aspects of infection of Babesia spp., in cattle and buffaloes, which must be taken into consideration when babesiosis control programs are implemented in the study area.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3091-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 76%

Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

et al. 2018

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“…T. orientalis is a cosmopolitan parasite of cattle that also affects buffaloes and yaks [8] [54][55][56], Africa [57][58][59], Europe [8,[60][61][62][63][64][65] and the Americas [1,10,66]. The distribution of Theileria species is dependent on the availability and competence of suitable tick vectors [7].…”

Section: Epidemiologymentioning

confidence: 99%

“…The majority of molecular distribution studies are based on the genetic characterisation of the T. orientalis MPSP gene. Some studies utilise other molecular markers such as the ITS 1, ITS 2, COX III and 18S rRNA genes to identify or characterise the parasite [46,66,99]. Studies based on molecular markers other than MPSP could not accurately classify MPSP genotypes, therefore, the identity of the MPSP genotypes found in some studies remain unclear [1].…”

Section: Global Distribution Of T Orientalismentioning

confidence: 99%

Oriental Theileriosis

Yam¹,

Bogema²,

Jenkins³

2019

Ticks and Tick-Borne Pathogens

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Theileria orientalis, the causative agent of oriental theileriosis, is an apicomplexan haemoparasite and is one of several tick-borne Theileria spp. infecting cattle. Unlike the highly pathogenic transforming Theileria species (T. annulata and T. parva) which induce uncontrolled lymphocytic proliferation, T. orientalis is a non-transforming strain exerting its major pathogenic effects via erythrocyte destruction. Clinical symptoms associated with oriental theileriosis are largely consequences of the underlying anaemia. Because of its non-transforming nature, T. orientalis was previously considered a benign parasite, however, in the recent years, clinical outbreaks of T. orientalis have been increasingly observed throughout Asia and Australasia. Recent rapid spread of clinical theileriosis has been linked to a pathogenic genotype of the parasite, genotype Ikeda (Type 2). The geographic distribution of clinical outbreaks correlates to the range of the major vector tick, Haemaphysalis longicornis, although other vectors and modes of transmission are possible. This review includes discussion of T. orientalis epidemiology, transmission, pathogenesis, treatment and control and provides an update on the taxonomy of this organism which is still under debate.

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“…infection in water buffaloes are still controversial (SIVAKUMAR et al, 2014), although fatal cases of theileriosis may occur during outbreaks (VINODKUMAR et al, 2016). In a previous study in water buffaloes from Brazil, Theileria infection was associated to lymphoproliferative disease (SILVEIRA et al, 2016). T. orientalis is distributed worldwide and is well known to cause clinical disease in cattle (WATTS et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Although clinical manifestations are not commonly observed in piroplasm-infected water buffaloes (Bubalus bubalis) (VINODKUMAR et al, 2016), a previous study reported a Theileria sp. related to the T. buffeli/ orientalis group that infects water buffaloes in the Brazilian Amazon region (SILVEIRA et al, 2016).…”

mentioning

confidence: 99%

Theileria sp. in water buffaloes from Maranhão State, northeastern Brazil

Abate

Santos

Brito³

et al. 2018

Rev. Bras. Parasitol. Vet.

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Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarcely reported in Brazil. Thus, the aim of this study was to screen water buffaloes from the state of Maranhão for piroplasm and A. marginale occurrence using PCR assays. All samples were negative for A. marginale. One of the 287 (0.35%) water buffaloes tested was positive for Theileria sp. Sequencing of the 18S rDNA fragment (356 bp) showed that the Theileria sp. identified was closely related to the T. buffeli /orientalis group. Future studies on the clinical signs of infection and the main vector in this country are needed.

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Molecular assays reveal the presence of Theileria spp. and Babesia spp. in Asian water buffaloes (Bubalus bubalis, Linnaeus, 1758) in the Amazon region of Brazil

Cited by 15 publications

References 25 publications

Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia

Oriental Theileriosis

Theileria sp. in water buffaloes from Maranhão State, northeastern Brazil

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