Molecular aspects of the mammalian cell surface

Mehrishi, J.N.

doi:10.1016/0079-6107(72)90013-2

Cited by 138 publications

(88 citation statements)

References 222 publications

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“…Most of the anionic sites on the eukaryotic cell surface result from the presence of carboxyl groups of acidic amino acids of proteins and glycoproteins (Weiss, 1969;Mehereshi, 1972), phosphate groups, and also carboxyl and sulphate residues of mucopolysaccharides (Burry & Wood, 1979). The carboxyl groups of sialic acids present in glycoproteins and glycolipids also seem to contribute strongly to the negative surface charge (James, 1979;Schauer, 1982).…”

Section: Virus Bindingmentioning

confidence: 99%

Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Soares¹,

Silva-Filho

Rozental

et al. 1998

Microbiology

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The surface anionogenic groups and sialoglycoconjugate structures of Paracoccidioides brasiliensis yeast forms were analysed by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion and flow cytof luorimetry. P. brasiliensis yeast forms, particularly the budding primordia, reacted strongly with cationized ferritin. Binding assays showed that the reaction with sialic-acid-specif ic Limax flaws lectin (LFA) was distributed over the entire P. brasiliensis cell wall. Treatment of yeast forms with neuraminidase significantly reduced their negative surface charge and LFA labelling, which suggests that sialic acid residues are major anionogenic groups exposed on the P. brasiliensis surface. Furthermore, after neuraminidase treatment, labelling with Arachis hypogaea (peanut) agglutinin increased due to unmasking of subterminal /I-D-galactopyranosyl residues. The sialic acid linkages to galactose are a2,6 and a283 as assessed, respectively, by fungal attachment to M1/5 and M1/5 HS8 strains of influenza A virus and binding of Sambucus niger and Maackia amurensis agglutinins. The d , 6 linkage clearly predominated in both experiments. Flow cytof luorimetry analysis revealed the heterogenicity of P. brasiliensis yeast cell populations, which comprised young and mature budding yeasts. Both express binding sites to LFA and Limulus polyphemus agglutinin.

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Section: Virus Bindingmentioning

confidence: 99%

Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Soares¹,

Silva-Filho

Rozental

et al. 1998

Microbiology

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“…As the electrical charge of the cell surface is predominantly due to the ionogenic groups of the carboxyl residues of sialic acids (Mehrishi, 1972), it is of interest to study the surface charge for a better understanding of the different stages of tumour development.…”

Section: Interactions Between Cell Surfacesmentioning

confidence: 99%

A comparative study of some growth characteristics and cell-surface properties of neoplastic cells

Bischoff¹,

Robert²,

Donner³

1981

Br J Cancer

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Summary.-Tumour cells from a polyoma-induced ascitic tumour were fractionated on the basis of the electrical charge on the cell surface by free-flow electrophoresis. Several characteristics of tumour cells have been investigated: (1) differences in the proliferation and antigenicity within the tumour at any point in time; (2) variation in proliferative potential with the ageing of the tumour.In early ascitic tumours, electrophoretically fractionated cells exhibit very similar proliferative characteristics. However, most DNA synthesis was found in slowmoving cells. The behaviour of older tumours was different. Proliferative potential and DNA synthesis were weaker and restricted to slow-moving cells, suggesting that fast-moving cells in older tumours were resting cells. An enrichment in immunoglobulin-bearing cells was also found in slow-moving cell fractions, supporting the hypothesis of variability in expression of tumour-specific antigens. The role of cellsurface properties and cell kinetics is discussed in relation to electrical surface charge, which might be involved in cell dissemination and metastasis. Thus, freeflow electrophoresis represents a satisfactory approach to isolate tumour cell subpopulations with characteristics such as high proliferative potential or increased expression of tumour antigens.

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“…The first, also denominated trans-membrane potential, is due to the semi-permeable characteristic of the biological membranes and is maintained by an energydependent mechanism. The second, also denominated cell surface charge, is due to a complex interaction between the polar groups residing at the cell surface with different ions of the surrounding medium (James 1979, Mehereish 1972, Weiss 1969.…”

Section: Surface Chargementioning

confidence: 99%

The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.

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Molecular aspects of the mammalian cell surface

Cited by 138 publications

References 222 publications

Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

A comparative study of some growth characteristics and cell-surface properties of neoplastic cells

The surface charge of trypanosomatids

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