Molecular approaches to taxonomy of fungi

Hall, Robert

doi:10.1007/bf02858874

Cited by 34 publications

(4 citation statements)

References 65 publications

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“…Fungal cell walls are constructed of polysaccharides which do not occur in higher plants. The primary constituents are chitin, a p-( I ,4)-polysaccharide derived from N-acetylglucosamine, and a mixture of non-cellulosic /3-~-( I ,3)-and /3-~-( I ,6)-glucans, along with smaller amounts of a-D-glucans and heteropolymers containing glucose, mannose, xylose and fucose (Aronson, 1965 ;Bartnicki-Garcia, 1968, 1970Gorin & Spencer, 1968;Hall, 1969). Cellulose, lignin and pectin are unknown from the higher macrofungi which are the primary concern of this review (Aronson, 1965;Bartnicki-Garcia, 1968, 1970.…”

Section: (I) Fungal Polysaccharidesmentioning

confidence: 99%

Biochemical Implications of Insect Mycophagy

Martin

1979

Biological Reviews

131

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Section: (I) Fungal Polysaccharidesmentioning

confidence: 99%

Biochemical Implications of Insect Mycophagy

Martin

1979

Biological Reviews

131

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“…One-dimensional gel electrophoresis (SDS-Page) of proteins, combined with computerized analysis of patterns, has been used increasingly in microbial Systematics (Barns et al, 1991;Hall, 1969;Hall et al, 1969;Johnston et al, 1988;Jordan, 1994;Magee et al, 1988;Meyer et al, 1993;Okiri & Kawamoto, 1995). Protein patterns offer considerable potential for typing strains of clinical interest and for taxonomic purposes, especially for the studies of biodiversity quantification among microorganisms (O' Donnell et al, 1994;Barns et al, 1991;Blignant & Koch, 1992;Booth, 1979).…”

Section: Introductionmentioning

confidence: 99%

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

et al. 2001

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The application of gel electrophoresis and numerical analysis of yeast soluble proteins analysis to the investigation of 12 oral yeast strains belonging to five species is described. It involves one-dimensional electrophoresis of SDS-solubilized whole-cell proteins using different culture media for the cultivation of the cells, integration densitometries in the areas of the gels and percentages of the proteins extraction. These extracts were prepared from four isolates of Candida albicans, two of C. tropicalis, C. guilliermondii, C. parapsilosis and C. krusei. The extracts from whole-cells proteins using different culture media for the cultivation of the cells were fractionated by slab electrophoresis using a discontinuous buffer system. The corresponding patterns showed at least 36 polypeptides in the range of 14.4-200 kDa. Different isolates of each species were clearly different in each of the five species. The data obtained suggest that different nutritional compositions led to the expression of different proteins derived from alternatives metabolic pathways expressed by the electrophoretic profiles. The construction of a database of protein fingerprints and numerical analysis based on such data, may have some implications in the classification and identification of such species with epidemiological, ecological and taxonomic purposes. A well defined or synthetic culture media seems to be much properly.

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“…This has presented considerable difficulties to freshwater ecologists and fish pathologists wishing to characterize and identify natural populations of Saprolegnia or to recognize potential pathogenic isolates (Neish, 1977 ;Willoughby, 1978). Recently, attempts have been made to use chemical information (reviewed by Hall, 1969) to resolve taxonomic and phylogenetic relationships within the Saprolegniales, including analysis of DNA (Green & Dick, 1972;Neish & Green, 1976) and cell wall amino acids (Vazira-Teherani & Dick, 1980a, b). Although revealing differences at the family level, these methods have been of little help in resolving the critical intrageneric relationships in the group.…”

Section: Introductionmentioning

confidence: 99%

Esterase Isoenzyme Variation in the Genus Saprolegnia, with Particular Reference to the Fish-pathogenic S. diclina-parasitica Complex

Beakes

Ford

1983

Microbiology

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Esterase isoenzyme patterns determined by slab gel electrophoresis were compared for nearly 60 Saprolegnia isolates, particularly from the S. diclina-parasitica complex. Consistent differences were found between S. diclina and S. parasitica isolates, with the latter being characterized by having between one and five very fast moving esterase bands that were generally absent from the former. A range of asexual (unidentifiable) isolates, taken from the vicinity of fish hatcheries or from fish lesions, were also examined and their esterase isoenzymes shown to be similar to those of S. parasitica, although usually showing fewer bands. The use of esterase isoenzymes for screening potential fish pathogenic isolates of Saprolegnia is briefly compared with results obtained using oogonium morphology or cyst ornamentation, and the relative merits of each method are discussed. It is proposed that, on the basis of their distinctive cyst coat ornamentation (bundles of long 'boathooks') and esterase isoenzymes, fish lesion isolates can be distinguished from saprophytic isolates, and that analysis of isoenzymes should provide a useful method for the screening of potential pathogenic isolates.

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Molecular approaches to taxonomy of fungi

Cited by 34 publications

References 65 publications

Biochemical Implications of Insect Mycophagy

Biochemical Implications of Insect Mycophagy

Differentiation and numerical analysis of oral yeasts based on SDS-Page profiles. Influence of the culture media on the whole-cell protein extracts

Esterase Isoenzyme Variation in the Genus Saprolegnia, with Particular Reference to the Fish-pathogenic S. diclina-parasitica Complex

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