Molecular approaches to improving the nutritional and functional properties of plant seeds as food sources: developments and comments

Lumen, Benito O. de

doi:10.1021/jf00099a001

Cited by 38 publications

(21 citation statements)

References 100 publications

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“…Seed proteins are coded by several homologous multigene families that vary in size (de Lumen 1990). Hence, the organization of proteins and protein fractions on electrophoregram makes an excellent fingerprint for discriminating between even morphologically similar genotypes.…”

Section: Discussionmentioning

confidence: 99%

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Panigrahi¹,

Kumar²,

Mishra³

et al. 2007

Plant Biotechnol Rep

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SDS-PAGE analysis of seed albumins and globulins from two pigeonpea, Cajanus cajan, cultivars (DSLR-17 and BDN-2) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, resulted in 34 albumin and 27 globulin polypeptides. Proximity matrix analysis based on electrophoretic banding patterns of albumins and globulins jointly revealed C. cajanifolius to be closest to C. cajan having similarity coefficients of 0.595 and 0.676, respectively. Cluster analysis also exhibited the grouping of C. cajanifolius with C. cajan in one cluster. C. platycarpus has been out grouped. The present analysis more or less agreed with the sectional classification of the genus Cajanus and it has been hypothesized that cultivated pigeonpea has evolved through multi-genomic interaction involving C. cajanifolius and that it has experienced minor genomic reorganization during its divergence.

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Section: Discussionmentioning

confidence: 99%

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Panigrahi¹,

Kumar²,

Mishra³

et al. 2007

Plant Biotechnol Rep

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“…Improvement of the nutritional quality of mungbean by increasing its methionine content will contribute to the improvement of the nutritional quality of the diet of people, especially the rural poor, in developing countries. Genetic engineering of legume storage proteins with the aim of improving nutritional quality and functional properties has been proposed (3)(4)(5)(6)(7). Soybean glycinins have been successfully engineered resulting in deletion of variable regions, insertion of tetramethionines in variable regions, deletion of disulfide bonds, and deletion and substitution of polyglutamic acid sequence with various amino acids, producing modified proglycinins with altered functionalities and nutritional values (5,6,(8)(9)(10)(11)(12)(13).…”

Section: Mungbean [Vigna Radiata (L)mentioning

confidence: 99%

Mungbean [Vigna radiata (L.) Wilczek] Globulins: Purification and Characterization

Mendoza

Adachi

Bernardo

et al. 2001

J. Agric. Food Chem.

112

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Vicilin type (8S) and basic 7S globulins and legumin type (11S) globulins were isolated from mungbean [Vigna radiata (L.) Wilczek]. The native molecular weights of the different globulin types were 360000 for legumin, 200000 for vicilin, and 135000 for basic 7S. Some of the 8S globulin apparently complexed and coeluted with the 11S on gel filtration. On SDS-PAGE, 11S was composed of two bands of 40000 and 24000, 8S was composed of 60000, 48000, 32000, and 26000 bands, and basic 7S was composed of 28000 and 16000 bands. The percent composition of total globulins was estimated to be as follow: 8S, 89%; basic 7S, 3.4%; and 11S, 7.6%. The basic 7S and 11S but not the 8S globulins were found to have disulfide bonds. The presence of carbohydrates by conjugated peroxidase reaction was observed in all bands of 8S, the acidic polypeptide of basic 7S, and its complex but not in 11S. The 28000 basic 7S band and its 42000 complex and the first three major bands of 8S cross-reacted with antibodies to all types of soybean conglycinin subunits (alpha, alpha', and beta), whereas the fourth band cross-reacted only with the anti-beta subunit. None of the mungbean globulins cross-reacted with anti-soybean glycinin. Basic 7S was found to be easily extracted with 0.15 M NaCl, 11S was extracted with 0.35 M NaCl,and 8S was extracted over a wide range of NaCl concentrations. The N-terminal sequences of the different subunits/fragments of the globulins were determined and found to have strong homology with storage proteins of other legumes and crops.

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“…New York for the nutrition of some hundred million people, particularly in developing countries (de Lumen, 1990;FAO, 1991). With respect to a rapidly increasing world population, international programmes for legume improvement have been started, including the search for wild species with resistance genes, as well as for cultivars and genotypes that can be genetically transformed and regenerated (de Lumen, 1990;Morrow, 1991;Shotwell and Larkins, 1991;Van Schoonhoven and Voysest, 1991;Christou, 1994). However, very little is known about the genome of Phaseolus species and related legumes, like Vigna.…”

Section: Introductionmentioning

confidence: 99%

A Flow Cytometric Analysis of the Nuclear 2C DNA Content in 17 Phaseolus Species (53 Genotypes)

Nagl

Treviranus

1995

Botanica Acta

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The genus Phaseolus is characterized by 1 highly stable karyotype of 2n = 22. Despite this con-;tancy, the size of the chromosomes varies, and crossing if species is possible only in a few cases. We determined ,he 2C nuclear DNA content of a number of Phaseolus species, cultivars and genotypes by flow cytometry, in xder to realize the interspecific and intraspecific variition of the 2C value. The data range from 1.03pg to !.18pg without any clear correlation to systematic reationships. The mean DNA values of wild and cultivated

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Molecular approaches to improving the nutritional and functional properties of plant seeds as food sources: developments and comments

Cited by 38 publications

References 100 publications

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Mungbean [Vigna radiata (L.) Wilczek] Globulins: Purification and Characterization

A Flow Cytometric Analysis of the Nuclear 2C DNA Content in 17 Phaseolus Species (53 Genotypes)

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