Molecular approaches to evaluate pollutants

Camatini, Marina; Bonfanti, P; Colombo, A; Urani, Chiara

doi:10.1016/s0045-6535(98)00316-6

Cited by 13 publications

(3 citation statements)

References 56 publications

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“…Several papers described the genotoxic effect of heavy metals and several organic substances in plants, based on comet assay or in micronucleus assays (Shaw et al, 1995;Camatini et al, 1998;Steinkellner et al, 1998). These approaches did not clarify the molecular mechanisms that induced DNA damage and are there as specific DNA target sequences.…”

Section: Discussionmentioning

confidence: 99%

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Labra

Fabio²,

Grassi

et al. 2003

Chemosphere

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In recent years several plant species have been in use as bioindicators and several tests have been developed to evaluate the toxicity of environmental pollutants in vegetal organisms. In the present paper Arabidopsis thaliana (L.) Heynh. (ecotype Wassilewskija) was used as bioindicators of two genotoxic substances: potassium dichromate and dihydrophenanthrene. Inhibition of seed germination was observed with both pollutants. AFLP analysis (i) indicated that both substances are genotoxic, (ii) showed that dihydrophenanthrene induces DNA changes in different target sequences than potassium dichromate, (iii) quantified the genotoxic effect using cluster analysis by comparing DNA from treated plants with that of control plants. On the basis of these considerations we suggest that AFLP method is a powerful tool for measuring qualitative and quantitative genotoxic activity due to environmental pollutants. AFLP method can be applied to a wide range of bioindicator organisms and may become a universal methodology to identify target genes for specific genotoxic agents. This could open up possibilities for designing specifically targeted assays and new approaches to risk assessment.

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Section: Discussionmentioning

confidence: 99%

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Labra

Fabio²,

Grassi

et al. 2003

Chemosphere

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“…MCPA induces cytochrome P450 isoenzymes in Hep G2 cells; the cytoskeletal components actin and vinculin were dramatically affected by MCPA metabolism cousing protein sulfhydryl groups depletion (Camatini et al 1998). Kobal and Budihna (1999) showed that at low concentration (15 mg kg -1 ) MCPA affects neither the body weight of adult rats and rabbits nor that of their offspring and the weight of inner organs is maintained.…”

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confidence: 99%

Toxicity of MCPA on non-green potato tuber calli

et al. 2008

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Growth of potato tuber calli cells (non-green) is inhibited by 4-chloro-2-methylphenoxyacetic acid (MCPA) as a consequence of perturbation of membrane integrity. MCPA also depresses ATP content with simultaneous increase of ADP and AMP, i.e., the energy charge is severely compromised. Cell redox state is also affected by MCPA, as a function of concentration. Up to 60 lM, MCPA stimulates glutathione reductase and glutathione transferase, whereas superoxide dismutase and catalase activities are not affected. However, 120 lM MCPA inhibits all these activities. Cell death challenged by MCPA is putatively related to disturbance of membrane integrity responsible for mitochondrial uncoupling with decrease of the energy charge and subsequent loss of ions and metabolites.

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“…32 Several studies focusing on chromosome aberrations such as comet assay micronucleus assays described the genotoxic effect of heavy metals and many organic substances. [33][34][35] These approaches do not provide sensitive information at DNA level as they analyse total nuclear DNA. On the other hand PCR-based molecular markers such as RAPD and AFLP (Amplified Fragment Length Polymorphism) are more sensitive as they give evidence about DNA mutation.…”

Section: Discussionmentioning

confidence: 99%

Assessment of air pollution genotoxicity by molecular markers in the exposed samples of Pseudevernia furfuracea (L.) Zopf in the province of Kayseri (Central Anatolia)

et al. 2010

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In living organisms heterogenous air pollutants are considered as a major source of DNA damage. In this study, we aimed to describe the DNA changes in Pseudevernia furfuracea (L.) Zopf samples exposed to pollution at various sites in Kayseri by RAPD (random amplified polymorphic DNA) analysis in order to reveal the pattern of genetic variation influenced by the environmental pollution. The study area is characterized by the presence of numerous industrial activities, such as steel works, glassworks, shipbuilding, and metallurgical, mechanical, chemical and food industries. Control samples were collected from Cat forest (Sivas) and exposed to pollution in dry and wet seasons in the province of Kayseri located in Central Anatolia. Results indicated that heterogeneous pollutants might have contributed to the changes in the band patterns obtained by RAPD analysis, reflecting the occurrence of DNA damage in the control samples.

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Molecular approaches to evaluate pollutants

Cited by 13 publications

References 56 publications

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Toxicity of MCPA on non-green potato tuber calli

Assessment of air pollution genotoxicity by molecular markers in the exposed samples of Pseudevernia furfuracea (L.) Zopf in the province of Kayseri (Central Anatolia)

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