Molecular approaches for AM fungal community ecology: A primer

Gorzelak, Monika A.; Holland, Taylor; Xing, Xiaoke; Hart, Miranda M.

doi:10.1016/j.mimet.2012.04.010

Cited by 27 publications

(19 citation statements)

References 104 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A current debate in the literature is how to most accurately measure fungal abundances (Gamper et al 2008, Tellenbach et al 2010, Shi et al 2012, Gorzelak et al 2012. Visual counts of colonization rates using staining/microscopic methods and qPCR measurements, which measure fungal gene copy numbers, can disagree (Duhamel et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Host plant quality mediates competition between arbuscular mycorrhizal fungi

Knegt

Jansa

Franken³

et al. 2016

Fungal Ecology

View full text Add to dashboard Cite

Host plant quality mediates competition between arbuscular mycorrhizal fungiKnegt, A.J.; Jansa, J.; Franken, O.; Engelmoer, D.J.P.; Werner, G.D.A.; Bücking, H.; Kiers, E.T. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. fungi, the intensity of this effect depended on the quality of the host, with the strongest differences found on low-quality (shaded) hosts. Our results suggest that environmental conditions for the host aboveground play a role in the competitive interactions among fungi belowground.

show abstract

Section: Discussionmentioning

confidence: 99%

Host plant quality mediates competition between arbuscular mycorrhizal fungi

Knegt

Jansa

Franken³

et al. 2016

Fungal Ecology

View full text Add to dashboard Cite

show abstract

“…The identification of AMF taxa in the environment is therefore not dependent on conventional culturing methods. Instead, molecular approaches (e.g., targeted polymerase chain reaction (PCR) followed by high-throughput amplicon sequencing) have become a widely used methodology for studying AMF communities (Gorzelak et al, 2012). …”

Section: Introductionmentioning

confidence: 99%

Impacts of Fertilization Regimes on Arbuscular Mycorrhizal Fungal (AMF) Community Composition Were Correlated with Organic Matter Composition in Maize Rhizosphere Soil

et al. 2016

View full text Add to dashboard Cite

The understanding of the response of arbuscular mycorrhizal fungi (AMF) community composition to fertilization is of great significance in sustainable agriculture. However, how fertilization influences AMF diversity and composition is not well-established yet. A field experiment located in northeast China in typical black soil (Chernozem) was conducted and high-throughput sequencing approach was used to investigate the effects of different fertilizations on the variation of AMF community in the rhizosphere soil of maize crop. The results showed that AMF diversity in the maize rhizosphere was significantly altered by different fertilization regimes. As revealed by redundancy analysis, the application of organic manure was the most important factor impacting AMF community composition between samples with and without organic manure, followed by N fertilizer and P fertilizer inputs. Moreover, the organic matter composition in the rhizosphere, determined by GC–MS, was significantly altered by the organic manure amendment. Many of the chemical components displayed significant relationships with the AMF community composition according to the Mantel test, among those, 2-ethylnaphthalene explained the highest percentage (54.2%) of the variation. The relative contents of 2-ethylnaphthalene and 2, 6, 10-trimethyltetradecane had a negative correlation with Glomus relative abundance, while the relative content of 3-methylbiphenyl displayed a positive correlation with Rhizophagus. The co-occurrence patterns in treatments with and without organic manure amendment were analyzed, and more hubs were detected in the network of soils with organic manure amendment. Additionally, three operational taxonomic units (OTUs) belonging to Glomerales were identified as hubs in all treatments, indicating these OTUs likely occupied broad ecological niches and were always active for mediating AMF species interaction in the maize rhizosphere. Taken together, impacts of fertilization regimes on AMF community composition were correlated with organic matter composition in maize rhizosphere soil and the application of manure could activate more AMF species to interact with other species in the maize rhizosphere. This knowledge can be valuable in regulating the symbiotic system of plants and AMF, maintaining the health and high yields of crops and providing a primary basis for rational fertilization.

show abstract

“…Molecular methods have become the standard for studying AMF communities (Gorzelak et al, 2012). Especially high throughput sequencing technologies such as 454 amplicon pyrosequencing (Margulies et al, 2005), enabling highly efficient characterization of microbial communities by sequencing medium-sized (200-600 bp) amplicons are currently often used (e.g.…”

Section: Introductionmentioning

confidence: 99%