Molecular and virological studies on contagious pustular dermatitis isolates from Egyptian sheep and goats

Mahmoud, Mohamed; Abdelrahman, Khaled A.; Soliman, Hatem

doi:10.1016/j.rvsc.2010.02.019

Cited by 25 publications

(21 citation statements)

References 30 publications

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“…At present, this disease is widespread in some major sheep and/or goat raising countries of Europe, America, and Africa, seriously threatening the development of the sheep/goat industry and human health over the world. (1,(33)(34)(35)(36)(37) In China, outbreaks of orf in provinces such as Qinghai, Xinjiang, Inner Mongolia, Jilin, and Heilongjiang (10,38) severely impact the sheep industry. This problem is most severe in Jilin Province.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses

Wang

Zhang

Hao

et al. 2015

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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Orf is caused by the orf virus (ORFV) and is a non-systemic, widespread disease afflicting sheep, goats, wild ruminants, and humans. Recent outbreaks in sheep and goats in Jilin and other northern Chinese provinces raise concerns about orf control in China. Thirty-five hybridoma clones were constructed from splenocytes of BALB/c mice immunized with natural orf virus protein. These hybridomas were used to produce antibodies targeting ORFV proteins. Immunological characterization of these monoclonal antibodies (MAb) showed that the 5F2D8 hybridoma line produced MAb that can recognize the 100, 70, and 20 kDa bands from total viral lysate. This hybridoma was further characterized by immunoprecipitation and peptide sequencing. The results indicate that 5F2D8 specifically recognizes orf virus encoded protein ORFV086, a late expression virion core protein that plays important roles in progeny virus particle assembly, morphogenesis, and maturity. Further experiments demonstrate that this MAb did not react with other viral proteins of ORFV orthopoxviruses, but reacted strongly to different field isolates of orf viruses from China. Additionally, this anti-ORFV086 MAb possesses ORFV neutralizing capability. Sequence alignments and phylogenetic analysis determined that ORFV086 of NA1/11, clustered together with NZ2 and IA82, is highly conserved and has structural similarities with the Vaccinia virus core protein P4a. As such, this MAb has great potential as a diagnostic tool for orf viruses, in the further exploration of orf pathogenesis, and in disease control and prevention.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses

Wang

Zhang

Hao

et al. 2015

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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“…The material from lesions of human infection was able to set untypical reaction in susceptible experimental sheep (Newsom and Cross, 1934;Lloyed et al, 1951;Mahmoud et al, 2010), lambs (Oppermann and Stumpke, 1937) and guinea pigs (Kozlowski and Dziekonski, 1956). Likewise, suspension of crusts from udder of goats and from the lesions of the woman and her child produced typical lesions when applied to scarified skin of young goats (Verdes et al, 1970).…”

Section: Diagnosis/confirmation Of Infectionmentioning

confidence: 99%

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Kumar¹,

Trivedi²,

Bhatt³

et al. 2015

Adv. Anim. Vet. Sci.

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| Contagious pustular dermatitis (CPD), also known as Orf or contagious ecthyma is an important viral disease of sheep and goats. It is mainly seen as a benign disease but malignant form has also been reported from few parts of the world. The rates of morbidity and mortality are higher, particularly in lambs and kids experiencing the disease for the first time. The causative agent of disease is Orf virus, type species of the genus Parapoxvirus belonging to family Poxviridae. The virus produces localized persistent proliferative skin lesions and affected hosts are infected repeatedly owing to its host immune evasive strategy. These viruses have been found uniformly labile to chloroform but resistant to ether and also exhibit antigenic variations among them. Clinically, the disease is enzootic and occurs in three forms viz. labial, genital / mammary and generalized forms. The incubation period in natural cases is 2-3 weeks. The disease outbreaks mostly occur between autumn and spring but its severity is more in autumn and winter than spring. The virus grows well in cell cultures of caprine, ovine and bovine origin. Confounding symptoms impose laboratory affirmation by serological assays or molecular techniques. The disease can be diagnosed by electron microscopy, serological tests and PCR/ quantitative real-time PCR. Virus specific antibody response to structural proteins of capripox and orf viruses in western-blot analysis readily differentiates these two infections. The antibody response to the 32 kDa and 26 kDa proteins of capripox viruses provides a firm basis for differentiation. Although, the role of humoral immunity is well established but probably cell mediated immunity plays a major role in recovery from natural infections. A number of inactivated and live or live modified vaccines have been tried with variable success. The duration of immunity after vaccination is controversial; outbreaks have occurred in vaccinated animals. The disease is also of public health significance as it causes infection in human beings. Keywords

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“…[25], Scabs, which were formed over the face, fatty tail and the rest of the body of the affected animals, were collected as a source of the virus and used for egg inoculation. The scabs were ground in 0.1 M sterile phosphate-buffered saline (PBS) containing antibiotics (penicillin 100 U/ml, streptomycin 100 µg/ml, and kanamycin 50 µg/ml).…”

Section: Methodsmentioning

confidence: 99%

Detection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt

Mahmoud

Khafagi

2016

Vet World

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Aim:To isolate, identify, and differentiate Capripoxviruses (CaPV) (sheep pox virus and goat pox virus) infections by egg inoculation, transmission electron microscopy (TEM), and 30 kDa RNA polymerase subunit gene-based polymerase chain reaction (PCR) (RPO30) in clinically affected animals in Hawamdia township of Giza Governorate, Egypt.Materials and Methods:A total of 37 scab samples were collected from clinically suspected field cases of sheep pox and goat pox. These samples were collected during (2014-2015) during different outbreaks of sheep pox and goat pox from Hawamdia township of Giza Governorate, Egypt. The samples were subjected to egg inoculation, TEM, and (RPO30) gene-based PCR. By using the egg inoculation: Previously prepared 37 scab samples (n=23 sheep and n=14 goats) were inoculated on the chorioallantoic membrane of specific pathogen free (SPF) embryonated chicken eggs (12 days old age). In the presence of the suitable percentage of humidity and candling, the inoculated eggs were incubated at 37°C. By using the TEM: Samples showed positive pock lesions on the chorioallantoic membranes, were fixed in glutaraldehyde, then processed and sectioned for TEM. Using the (RPO30) gene-based PCR assay, 30 of positive samples after egg inoculation (n=19 sheep and n=11 goats) were screened.Results:Using the egg inoculation, a characteristic pock lesions for poxviruses were seen in 30/37 (n=19 sheep and n=11 goats) (81.08%). Using the TEM, examination of the positive samples after egg inoculation revealed positive result in 23/30 (n=15 sheep and n=8 goats) (76.66%). The positive results represented by the presence of negatively stained oval-shape virus particles. Using the (RPO30) gene-based PCR assay, out of 30 total of positive samples after egg inoculation (n=19 sheep and n=11 goats) were screened, 27 (90%) samples (n=17 sheep and n=10 goats) were positive. The given band sizes of sheep and goats were 172 and 152 bp, respectively.Conclusion:PCR assay depended on RPO30 gene can be used lonely for the detection, identification, and differentiation of CaPVs. RPO30 gene-based PCR assay in combination with gene sequencing helps in molecular epidemiological studies of CaPV infection.

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Molecular and virological studies on contagious pustular dermatitis isolates from Egyptian sheep and goats

Cited by 25 publications

References 30 publications

Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses

Isolation and Characterization of Monoclonal Antibodies Against a Virion Core Protein of Orf Virus Strain NA1/11 As Potential Diagnostic Tool for Orf Viruses

Contagious Pustular Dermatitis (Orf Disease) - Epidemiology, Diagnosis, Control and Public Health Concerns

Detection, identification, and differentiation of sheep pox virus and goat pox virus from clinical cases in Giza Governorate, Egypt

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