Molecular and symbiotic characterization of peanut bradyrhizobia from the semi-arid region of Brazil

Santos, Jonnathan Whiny Moraes dos; Silva, Jéssica Fernanda da; Ferreira, tainá Dourado; Dias, Marcos André Moura; Fraiz, Ana Carla Resende; Escobar, Indra Elena Costa; Santos, Roseane Cavalcanti dos; Lima, Liziane Maria de; Morgante, Carolina Vianna; Fernandes‐Júnior, Paulo Ivan

doi:10.1016/j.apsoil.2017.09.033

Cited by 22 publications

(9 citation statements)

References 39 publications

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“…In general, the prevalence of B. japonicum superclade among the evaluated isolates is clear, as reported by other authors who studied the diversity of rhizobia associated with cowpeas (Sarr et al 2011;Chidebe et al 2018; Jaiswal and Dakor a 2019 ), and has be en obse rved i n other Bradyrhizobium diversity studies in the Brazilian semiarid region (Santos et al 2017) in agricultural managed soils.…”

Section: Discussionsupporting

confidence: 79%

“…Many studies have sought to determine the diversity of native nitrogen-fixing bacteria in Brazilian dry regions (Martins et al 2003;Leite et al 2009;Marinho et al 2017;Santos et al 2017;Rodrigues et al 2018), such as in the Lower Half São Francisco River Valley (Leite et al 2009;Nunes et al 2018). However, these studies did not consider the heterogeneity of the agricultural systems in the region.…”

Section: Introductionmentioning

confidence: 99%

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Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

Sena

Nascimento

Lino

et al. 2020

J Soil Sci Plant Nutr

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The objectives of this study were to characterize the genetic diversity and evaluate the ability to tolerate stress as well as to assess the symbiotic efficiency of bacteria from cowpea nodules in agricultural soils with different uses in the semiarid region of Bahia state (Brazil). Soil samples were collected from six crop lands and one from the pristine Caatinga biome. After a trap-host experiment, the bacteria were isolated and culturally characterized. Isolates with typical characteristics of Bradyrhizobium were subjected to the nodC symbiotic gene amplification and those positive were evaluated by 16S-23S IGS-RFLP. Twenty-seven isolates belonging to different genetic clusters were selected for 16S-23S IGS sequencing. In additions, the selected bacteria were characterized biochemically and symbiotically. Among 420 characterized isolates, approximately 60% (251 isolates) displayed typical Bradyrhizobium cultural features. A total of 161, out of 251 isolates, showed positive amplification of the nodC gene fragment. The IGS-RFLP profiles analysis generated 33 groups and 27 were selected for further analysis. The fertility of the soils influenced the distribution of the isolates in the IGS-RFLP clusters. The bacteria were assigned to two genera, Bradyrhizobium and Microvirga, with 26 and 1 representative bacteria, respectively. Some isolates were able to tolerate NaCl as well as acidic and alkaline pH. In addition, isolates showed the abilities to produce biofilm under stress and to produce indole compounds, as well as efficient nodulation and nitrogen fixation. The isolates displayed great genetic, biochemical, and symbiotic variability with promising biotechnological potential.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

Sena

Nascimento

Lino

et al. 2020

J Soil Sci Plant Nutr

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“…Plant-growth-promoting rhizobacteria, such as rhizobia, can stimulate root growth, which in addition to the Biological Nitrogen Fixation (BNF) by several other mechanisms. The growth promotion of peanut roots by ESA 123 was already observed (Santos et al, 2017), even under water stress (Barbosa et al, 2018), indicating a beneficial feature of this strain, in addition to BNF.…”

Section: Resultsmentioning

confidence: 75%

“…In addition to initiatives to generate new genotypes, research groups have focused on the selection of rhizobia adapted to soil and semiarid climatic conditions. These studies have led to the selection of efficient rhizobia strains both under greenhouse conditions as well as in the field (Sizenando et al, 2016;Santos et al, 2017). Rhizobia can improve legume tolerance to drought as already shown for peanuts (Furlan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Brito¹,

Santos²,

Barbosa³

et al. 2019

Genet. Mol. Res.

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The peanut plant has high plasticity and great adaptability to adverse conditions, including drought. To mitigate the negative effects of drought on legumes, nitrogen-fixing microorganisms have been investigated in some plant species, such as soybeans and beans. We analyzed the role Bradyrhizobium strains in peanut genotypes subjected to water deficit by means of plant growth, physiological and gene expression analysis. The research was conducted under greenhouse conditions with the runner peanut genotypes (IAC Runner 886, 2012-33 and 2012-47) and two Bradyrhizobium strains (ESA 123 and SEMIA 6144). After 20 days of germination, the water supply was completely interrupted and gas exchange analysis were carried out using an infrared gas analyzer, up to the 10 th day of stress. Leaves were collected for the analysis of proline content and the expression of NCED and ERF8 genes. Analyses of plant height (PH), shoot dry mass (SDM), root dry mass (RDM), number of nodules (NN) and nodule weight (NW) also were ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (4): gmr18379 S.L. Brito et al. 2 performed. The peanut shoots inoculated with the Bradyrhizobium strains obtained the best results. The genotypes inoculated with the ESA 123 strain obtained superior responses compared to the nonstressed treatment inoculated with the same bacteria and the stressed control without bacterial inoculation. In the ESA 123 inoculated treatments, the water stressed plants had higher RDM (28.5% higher, on average), NN (two fold higher), and gene expression (approximately six and threefold higher for ERF8 and NCED genes, respectively). The increase in the expression of NCED and ERF8 genes, in the three genotypes inoculated with ESA 123, suggests a key role of this inoculant in the activation of metabolic cascades for plant protection under water deficit.

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“…Despite being members of the Bradyrhizobium genus (according to IGS sequencing), based on the bacterial BOX-PCR profiles, the isolates from the large clusters with 100% similarity showed great intraspecific variability, with few clonal isolates among the bacteria within the culture collection. BOX-PCR is a fingerprinting technique with a high ability to discriminate among bacterial isolates within the same species or close species, including the Bradyrhizobium genus (Santos et al 2017;Chidebe et al 2018), and exploit the diversity of rhizobial culture collections. In addition to being very diverse, both bacteria from soils with and without biochar were symbiotically efficient compared with the reference strain B. yuanmingense BR 3267.…”

Section: Discussionmentioning

confidence: 99%

Are Cowpea-Nodulating Bradyrhizobial Communities Influenced by Biochar Amendments in Soils? Genetic Diversity and Symbiotic Effectiveness Assessment of Two Agricultural Soils of Brazilian Drylands

Oliveira

Sena

Nascimento

et al. 2019

J Soil Sci Plant Nutr

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The objective of this study was to evaluate the influence of biochar amendment on the bacterial genetic diversity and symbiosis in cowpea-nodulating rhizobial communities in two agricultural soils of Brazilian drylands. Vertisol and Ultisol surface layer soil samples were collected in the Brazilian semiarid region. The soils were mixed with biochar and cowpea seeds (BRS Marataoã) and sown in pots. Forty-five days after plant emergence, the nodule bacteria were isolated. The genetic variability of the nodule bacteria was evaluated by means of restriction fragment length polymorphism of 16S-23S rRNA intergenic spacer region (IGS-RFLP), and those that shared identical IGS-RFLP profiles were fingerprinted by BOX-PCR. By selecting of representative isolates in the genetic clusters, 27 bacteria were identified by 16S-23S IGS sequencing and symbiotically assessed. The IGS-RFLP dendrogram of the 73 isolates had 20 clusters; the multivariate correspondence analysis grouped both Vertisol treatments, and the Ultisol treatments were placed far away in the biplot. Ultisol showed higher bradyrhizobial diversity than the Vertisol. The fingerprinting indicated the presence of only three clonal colonies, showing high intraspecific diversity. The 16S-23S IGS sequences indicated the prevalence of bacteria related to Bradyrhizobium guangxiense in the Vertisol and Bradyrhizobium zhanjiangense in the Ultisol, in addition to the presence of three putative Microvirga spp. in the Ultisol. The symbiotic efficiency of 22 out of 27 bacteria was comparable with that of the inoculant strain Bradyrhizobium yuanmingense BR 3267. The genetic diversity of the cowpea-nodulating bradyrhizobia was not primarily affected by the biochar application in two agricultural soils of Brazilian drylands. Both soils harbored symbiotically efficient bacteria, without the influence of biochar amendment .

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Molecular and symbiotic characterization of peanut bradyrhizobia from the semi-arid region of Brazil

Cited by 22 publications

References 39 publications

Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

Molecular, Physiological, and Symbiotic Characterization of Cowpea Rhizobia from Soils Under Different Agricultural Systems in the Semiarid Region of Brazil

Research Article <i>Bradyrhizobium</i> spp. as attenuators of water deficit stress in runner peanut genotypes based on physiological and gene expression responses

Are Cowpea-Nodulating Bradyrhizobial Communities Influenced by Biochar Amendments in Soils? Genetic Diversity and Symbiotic Effectiveness Assessment of Two Agricultural Soils of Brazilian Drylands

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